Item talk:Q59400
From geokb
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"name": "Exposure-related effects of formulated Pseudomonas fluorescens strain CL145A to glochidia from seven unionid mussel species",
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"abstract": "The study was completed to evaluate the exposure-related effects of a biopesticide for dreissenid mussel (Dreissena polymorpha, zebra mussel and\u00a0Dreissena rostriformis bugensis, quagga mussel) control on glochidia from unionid mussels endemic to the Great Lakes and Upper Mississippi River Basins. The commercially prepared biopesticide was either a spray-dried powder (SDP) or freeze-dried powder (FDP) formulation of\u00a0Pseudomonas fluorescens, strain CL145A. Glochidia of the unionid mussel species\u00a0Lampsilis cardium,\u00a0Lampsilis siliquoidea,Lampsilis higginsii,\u00a0Ligumia recta,\u00a0Obovaria olivaria, and\u00a0Actinonaias ligamentina\u00a0were exposed to SDP-formulated\u00a0P. fluorescens\u00a0andLampsilis cardium\u00a0and\u00a0Megalonaias nervosa\u00a0were exposed to FDP-formulated\u00a0P. fluorescens.\nAll exposures were static, 24 hours in duration, and included six treatment groups. The treatment groups included (1) an untreated control, (2) a positive control which received a nominal target active ingredient (AI) concentration of 300 milligrams per liter (mg/L) of heat-deactivated test article, and (3) treatments that received nominal target AI concentrations of 50, 100, 200, and 300 mg/L of test article. All treatment concentrations are reported based on active ingredient.\nGlochidia viability was reduced in two of the six species exposed to 50 mg/L SDP and in four of the six species exposed to 100 mg/L SDP when compared to untreated control groups at 6, 12, and 24 hours. Regardless of sample time, concentrations of 200 and 300 mg/L of SDP and 300 mg/L of heat-deactivated SDP (positive control) substantially reduced glochidia viability in all species except,\u00a0L.\u00a0higginsii. Glochidia viability was only reduced for L. cardium exposed to FDP at concentrations \u2265 200 mg/L. After 24 hours of FDP exposure, differences in glochidia viability were only detected in\u00a0M.\u00a0nervosa\u00a0that were exposed to 300 mg/L of heat-deactivated SDP. However, given the low viability in the control group, the results for\u00a0M.\u00a0nervosa\u00a0should be interpreted with caution.",
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