Item talk:Q318255

{

 "DOI": {
   "doi": "10.5066/p1qvr9e4",
   "identifiers": [],
   "creators": [
     {
       "name": "Daniel A Grear",
       "nameType": "Personal",
       "affiliation": [
         "United States Geological Survey"
       ],
       "nameIdentifiers": [
         {
           "schemeUri": "https://orcid.org",
           "nameIdentifier": "https://orcid.org/0000-0002-5478-1549",
           "nameIdentifierScheme": "ORCID"
         }
       ]
     }
   ],
   "titles": [
     {
       "title": "Data Release for Replicate amphibian swab and standard dilution of artificial DNA data to assess qPCR and sampling detection error for Batrachochytrium dendrobatidis."
     }
   ],
   "publisher": "U.S. Geological Survey",
   "container": {},
   "publicationYear": 2024,
   "subjects": [
     {
       "subject": "biota"
     },
     {
       "subject": "Wildlife Disease"
     }
   ],
   "contributors": [],
   "dates": [],
   "language": null,
   "types": {
     "ris": "DATA",
     "bibtex": "misc",
     "citeproc": "dataset",
     "schemaOrg": "Dataset",
     "resourceType": "Dataset",
     "resourceTypeGeneral": "Dataset"
   },
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   "formats": [
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     "csv"
   ],
   "version": null,
   "rightsList": [
     {
       "rights": "Creative Commons Zero v1.0 Universal",
       "rightsUri": "https://creativecommons.org/publicdomain/zero/1.0/legalcode",
       "schemeUri": "https://spdx.org/licenses/",
       "rightsIdentifier": "cc0-1.0",
       "rightsIdentifierScheme": "SPDX"
     }
   ],
   "descriptions": [
     {
       "description": "We generated a data set from by collecting duplicate swabs from all amphibian individuals sampled at 5 sites (96 individuals) and analyzed these duplicate swabs running 3 subsamples of the extracted DNA in separate qPCR reactions. The resulting data set included 2 swabs per individual with 1 qPCR result and a 2nd swab with 3 qPCR results for detection of Batrachochytrium dendrobatidis (Bd) DNA. We generated data to assess the ideal laboratory functional relationship between quantity of target Bd DNA and detection, as well as limits of detection (LOD) and quantification (LOQ) for the assay, 5 \u00b5L of synthetic double-stranded Bd DNA template (gBlocks\u2122 Gene Fragments, Integrated DNA Technologies, Coralville, IA) ranging from 1.46 x 10-3 fg to 3 fg (approximately 6.6 \u2013 13,000 copies) per reaction at 2-fold dilutions were each run 80 times on 10 separate plates (12 dilution series per plate).",
       "descriptionType": "Abstract"
     }
   ],
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   "url": "https://www.sciencebase.gov/catalog/item/66a02911d34eef99d5abc7a9",
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   "created": "2024-07-25T23:30:34Z",
   "registered": "2024-07-25T23:30:34Z",
   "published": null,
   "updated": "2024-07-25T23:30:36Z"
 }

}