Item talk:Q313875
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{
"USGS Publications Warehouse": { "@context": "https://schema.org", "@type": "Article", "additionalType": "Journal Article", "name": "Using the electron transport system as an indicator of organismal thermal tolerance and respiratory exploitation", "identifier": [ { "@type": "PropertyValue", "propertyID": "USGS Publications Warehouse IndexID", "value": "70256469", "url": "https://pubs.usgs.gov/publication/70256469" }, { "@type": "PropertyValue", "propertyID": "USGS Publications Warehouse Internal ID", "value": 70256469 }, { "@type": "PropertyValue", "propertyID": "DOI", "value": "10.1139/cjz-2023-0027", "url": "https://doi.org/10.1139/cjz-2023-0027" } ], "journal": { "@type": "Periodical", "name": "Canadian Journal of Zoology", "volumeNumber": "102", "issueNumber": "2" }, "inLanguage": "en", "isPartOf": [ { "@type": "CreativeWorkSeries", "name": "Canadian Journal of Zoology" } ], "datePublished": "2024", "dateModified": "2024-08-05", "abstract": "Freshwater ecosystems are undergoing rapid thermal shifts, making it increasingly important to understand species-specific responses to these changes. Traditional techniques for determining a species\u2019 thermal tolerance are often lethal and time consuming. Using the enzyme activity associated with the electron transport system (ETS; hereafter referred to as enzyme assay) may provide a non-lethal, rapid, and efficient alternative to traditional techniques for some species. We used largemouth bass\u00a0Micropterus salmoides\u00a0(Lacepede, 1802) to test the efficacy of using an enzyme assay to determine thermal tolerance and respiratory exploitation in response to variable acclimation temperatures. Three tissue types were dissected from fish acclimated to 20, 25, or 30\u2009\u00b0C and used in ETS assays at temperatures ranging from 7.5 to 40\u2009\u00b0C. While there were significant differences among tissue types and acclimation temperatures, maximal enzyme activity occurred from 25.23 to 31.91\u2009\u00b0C. Fish lost equilibrium at 39\u201342\u2009\u00b0C in traditional CTmax\u00a0trials, significantly higher than the upper optimum range determined via enzyme assays. The ratio of enzyme activity to measured whole organism respiration rate decreased with increasing water temperature, with the largest changes occurring at the upper optimum thermal range determined by enzyme assays. Our results indicate that ETS analysis may prove useful for obtaining biologically relevant thermal tolerances.", "description": "11 p.", "publisher": { "@type": "Organization", "name": "Canadian Science Publishing" }, "author": [ { "@type": "Person", "name": "Stell, Ehlana G.", "givenName": "Ehlana G.", "familyName": "Stell", "affiliation": [ { "@type": "Organization", "name": "Auburn University" } ] }, { "@type": "Person", "name": "Brewer, Shannon K. skbrewer@usgs.gov", "givenName": "Shannon K.", "familyName": "Brewer", "email": "skbrewer@usgs.gov", "identifier": { "@type": "PropertyValue", "propertyID": "ORCID", "value": "0000-0002-1537-3921", "url": "https://orcid.org/0000-0002-1537-3921" }, "affiliation": [ { "@type": "Organization", "name": "Fort Collins Science Center", "url": "https://www.usgs.gov/centers/fort-collins-science-center" }, { "@type": "Organization", "name": "Coop Res Unit Atlanta", "url": "https://www1.usgs.gov/coopunits/unit/Georgia" } ] }, { "@type": "Person", "name": "Horne, Lindsay M.", "givenName": "Lindsay M.", "familyName": "Horne", "affiliation": [ { "@type": "Organization", "name": "Lincoln Memorial University" } ] }, { "@type": "Person", "name": "Wright, Russell A.", "givenName": "Russell A.", "familyName": "Wright", "affiliation": [ { "@type": "Organization", "name": "Auburn University" } ] }, { "@type": "Person", "name": "DeVries, Dennis R.", "givenName": "Dennis R.", "familyName": "DeVries", "affiliation": [ { "@type": "Organization", "name": "Auburn University" } ] } ], "funder": [ { "@type": "Organization", "name": "Coop Res Unit Atlanta", "url": "https://www1.usgs.gov/coopunits/unit/Georgia" } ] }, "OpenAlex": { "_id": "https://openalex.org/w4386100598", "abstract_inverted_index": { "Freshwater": [ 0 ], "ecosystems": [ 1 ], "are": [ 2, 26 ], "undergoing": [ 3 ], "rapid": [ 4 ], "thermal": [ 5, 24, 82, 187, 206 ], "shifts,": [ 6 ], "making": [ 7 ], "it": [ 8 ], "increasingly": [ 9 ], "important": [ 10 ], "to": [ 11, 15, 45, 57, 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"Fish": [ 140 ], "lost": [ 141 ], "equilibrium": [ 142 ], "39\u201342": [ 144 ], "CT": [ 148 ], "max": [ 149 ], "trials,": [ 150 ], "significantly": [ 151 ], "higher": [ 152 ], "than": [ 153 ], "upper": [ 155, 185 ], "optimum": [ 156, 186 ], "range": [ 157, 188 ], "determined": [ 158, 189 ], "via": [ 159 ], "assays.": [ 161, 192 ], "The": [ 162 ], "ratio": [ 163 ], "measured": [ 168 ], "whole": [ 169 ], "organism": [ 170 ], "respiration": [ 171 ], "rate": [ 172 ], "decreased": [ 173 ], "increasing": [ 175 ], "water": [ 176 ], "temperature,": [ 177 ], "largest": [ 180 ], "changes": [ 181 ], "occurring": [ 182 ], "by": [ 190 ], "Our": [ 193 ], "results": [ 194 ], "indicate": [ 195 ], "that": [ 196 ], "analysis": [ 198 ], "prove": [ 200 ], "useful": [ 201 ], "obtaining": [ 203 ], "biologically": [ 204 ], "relevant": [ 205 ], "tolerances.": [ 207 ] }, "apc_list": null, "apc_paid": null, "authorships": [ { "author_position": "first", "author": { "id": "https://openalex.org/A5066135296", 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