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{
"USGS Publications Warehouse": { "@context": "https://schema.org", "@type": "Article", "additionalType": "Journal Article", "name": "An enriched stable-isotope approach to determine the gill-zinc binding properties of juvenile rainbow trout (Oncorhynchus mykiss) during acute zinc exposures in hard and soft waters", "identifier": [ { "@type": "PropertyValue", "propertyID": "USGS Publications Warehouse IndexID", "value": "70034397", "url": "https://pubs.usgs.gov/publication/70034397" }, { "@type": "PropertyValue", "propertyID": "USGS Publications Warehouse Internal ID", "value": 70034397 }, { "@type": "PropertyValue", "propertyID": "DOI", "value": "10.1897/08-252.1", "url": "https://doi.org/10.1897/08-252.1" }, { "@type": "PropertyValue", "propertyID": "ISSN", "value": "07307268" } ], "journal": { "@type": "Periodical", "name": "Environmental Toxicology and Chemistry", "volumeNumber": "28", "issueNumber": "6" }, "inLanguage": "en", "isPartOf": [ { "@type": "CreativeWorkSeries", "name": "Environmental Toxicology and Chemistry" } ], "datePublished": "2009", "dateModified": "2012-03-12", "abstract": "The objective of the present study was to employ an enriched stable-isotope approach to characterize Zn uptake in the gills of rainbow trout (Oncorhynchus mykiss) during acute Zn exposures in hard water (???140 mg/L as CaCO 3) and soft water (???30 mg/L as CaCO3). Juvenile rainbow trout were acclimated to the test hardnesses and then exposed for up to 72 h in static exposures to a range of Zn concentrations in hard water (0-1,000 ??g/L) and soft water (0-250 ??g/L). To facilitate detection of new gill Zn from endogenous gill Zn, the exposure media was significantly enriched with 67Zn stable isotope (89.60% vs 4.1% natural abundance). Additionally, acute Zn toxicity thresholds (96-h median lethal concentration [LC50]) were determined experimentally through traditional, flow-through toxicity tests in hard water (580 ??g/L) and soft water (110 ??g/L). Following short-term (???3 h) exposures, significant differences in gill accumulation of Zn between hard and soft water treatments were observed at the three common concentrations (75, 150, and 250 ??g/L), with soft water gills accumulating more Zn than hard water gills. Short-term gill Zn accumulation at hard and soft water LC50s (45-min median lethal accumulation) was similar (0.27 and 0.20 ??g/g wet wt, respectively). Finally, comparison of experimental gill Zn accumulation, with accumulation predicted by the biotic ligand model, demonstrated that model output reflected short-term (<1 h) experimental gill Zn accumulation and predicted observed differences in accumulation between hard and soft water rainbow trout gills. Our results indicate that measurable differences exist in short-term gill Zn accumulation following acclimation and exposure in different water hardnesses and that short-term Zn accumulation appears to be predictive of Zn acute toxicity thresholds (96-h LC50s). ?? 2009 SETAC.", "publisher": { "@type": "Organization", "name": "U.S. Geological Survey" }, "author": [ { "@type": "Person", "name": "Wolf, R.E.", "givenName": "R.E.", "familyName": "Wolf" }, { "@type": "Person", "name": "Lamothe, P. J.", "givenName": "P. J.", "familyName": "Lamothe" }, { "@type": "Person", "name": "Smith, K. S.", "givenName": "K. S.", "familyName": "Smith", "identifier": { "@type": "PropertyValue", "propertyID": "ORCID", "value": "0000-0001-8547-9804", "url": "https://orcid.org/0000-0001-8547-9804" } }, { "@type": "Person", "name": "Todd, A.S.", "givenName": "A.S.", "familyName": "Todd" }, { "@type": "Person", "name": "Ranville, J. F.", "givenName": "J. F.", "familyName": "Ranville" }, { "@type": "Person", "name": "Brinkman, S.", "givenName": "S.", "familyName": "Brinkman" } ] }, "OpenAlex": { "_id": "https://openalex.org/w2163249431", "abstract_inverted_index": { "The": [ 0 ], "objective": [ 1 ], "of": [ 2, 20, 68, 84, 147, 203, 271 ], "the": [ 3, 18, 51, 92, 158, 212 ], "present": [ 4 ], "study": [ 5 ], "was": [ 6, 95, 192 ], "to": [ 7, 13, 50, 59, 65, 268 ], "employ": [ 8 ], "an": [ 9 ], "enriched": [ 10, 97 ], "stable-isotope": [ 11 ], "approach": [ 12 ], "characterize": [ 14 ], "Zn": [ 15, 27, 69, 87, 109, 148, 173, 180, 206, 226, 252, 265, 272 ], "uptake": [ 16 ], "in": [ 17, 29, 62, 71, 125, 144, 232, 249, 258 ], "gills": [ 19, 170 ], "rainbow": [ 21, 46, 239 ], "trout": [ 22, 47, 240 ], "(Oncorhynchus": [ 23 ], "mykiss)": [ 24 ], "during": [ 25 ], "acute": [ 26, 108, 273 ], "exposures": [ 28, 64 ], "hard": [ 30, 72, 126, 150, 175, 183, 235 ], "water": [ 31, 39, 73, 78, 127, 132, 153, 169, 176, 186, 238, 260 ], "(approximately": [ 32, 40 ], "140": [ 33 ], "mg/L": [ 34, 42 ], "as": [ 35, 43 ], "CaCO3)": [ 36 ], "and": [ 37, 54, 76, 130, 151, 164, 184, 195, 228, 236, 256, 262 ], "soft": [ 38, 77, 131, 152, 168, 185, 237 ], "30": [ 41 ], "CaCO3).": [ 44 ], "Juvenile": [ 45 ], "were": [ 48, 117, 155 ], "acclimated": [ 49 ], "test": [ 52 ], "hardnesses": [ 53, 261 ], "then": [ 55 ], "exposed": [ 56 ], "for": [ 57 ], "up": [ 58 ], "72": [ 60 ], "h": [ 61 ], "static": [ 63 ], "a": [ 66 ], "range": [ 67 ], "concentrations": [ 70, 161 ], "(0-1000": [ 74 ], "microg/L)": [ 75, 129 ], "(0-250": [ 79 ], "microg/L).": [ 80, 134 ], "To": [ 81 ], "facilitate": [ 82 ], "detection": [ 83 ], "new": [ 85 ], "gill": [ 86, 90, 145, 179, 205, 225, 251 ], "from": [ 88 ], "endogenous": [ 89 ], "Zn,": [ 91 ], "exposure": [ 93, 257 ], "media": [ 94 ], "significantly": [ 96 ], "with": [ 98, 167, 208 ], "67Zn": [ 99 ], "stable": [ 100 ], "isotope": [ 101 ], "(89.60%": [ 102 ], "vs.": [ 103 ], "4.1%": [ 104 ], "natural": [ 105 ], "abundance).": [ 106 ], "Additionally,": [ 107 ], "toxicity": [ 110, 123, 274 ], "thresholds": [ 111, 275 ], "(96-h": [ 112, 276 ], "median": [ 113, 189 ], "lethal": [ 114, 190 ], "concentration": [ 115 ], "[LC50])": [ 116 ], "determined": [ 118 ], "experimentally": [ 119 ], "through": [ 120 ], "traditional,": [ 121 ], "flow-through": [ 122 ], "tests": [ 124 ], "(580": [ 128 ], "(110": [ 133 ], "Following": [ 135 ], "short-term": [ 136, 221, 250, 264 ], "(<": [ 137 ], "or": [ 138 ], "=3": [ 139 ], "h)": [ 140, 223 ], "exposures,": [ 141 ], "significant": [ 142 ], "differences": [ 143, 231, 247 ], "accumulation": [ 146, 181, 209, 227, 233, 253, 266 ], "between": [ 149, 234 ], "treatments": [ 154 ], "observed": [ 156, 230 ], "at": [ 157, 182 ], "three": [ 159 ], "common": [ 160 ], "(75,": [ 162 ], "150,": [ 163 ], "250": [ 165 ], "microg/L),": [ 166 ], "accumulating": [ 171 ], "more": [ 172 ], "than": [ 174 ], "gills.": [ 177, 241 ], "Short-term": [ 178 ], "LCS0s": [ 187 ], "(45-min": [ 188 ], "accumulation)": [ 191 ], "similar": [ 193 ], "(0.27": [ 194 ], "0.20": [ 196 ], "microg/g": [ 197 ], "wet": [ 198 ], "wt,": [ 199 ], "respectively).": [ 200 ], "Finally,": [ 201 ], "comparison": [ 202 ], "experimental": [ 204, 224 ], "accumulation,": [ 207 ], "predicted": [ 210, 229 ], "by": [ 211 ], "biotic": [ 213 ], "ligand": [ 214 ], "model,": [ 215 ], "demonstrated": [ 216 ], "that": [ 217, 245, 263 ], "model": [ 218 ], "output": [ 219 ], "reflected": [ 220 ], "(<1": [ 222 ], "Our": [ 242 ], "results": [ 243 ], "indicate": [ 244 ], "measurable": [ 246 ], "exist": [ 248 ], "following": [ 254 ], "acclimation": [ 255 ], "different": [ 259 ], "appears": [ 267 ], "be": [ 269 ], "predictive": [ 270 ], "LC50s).": [ 277 ] }, "apc_list": { "value": 3400, "currency": "USD", "value_usd": 3400, "provenance": "doaj" }, "apc_paid": null, "authorships": [ { "author_position": "first", "author": { "id": "https://openalex.org/A5091072817", "display_name": "Andrew S. 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