Item talk:Q302127
From geokb
{
"USGS Publications Warehouse": { "@context": "https://schema.org", "@type": "Article", "additionalType": "Journal Article", "name": "Considerations for colorblind individuals on selecting colorimetric or fluorescent dye assay outcomes", "identifier": [ { "@type": "PropertyValue", "propertyID": "USGS Publications Warehouse IndexID", "value": "70249990", "url": "https://pubs.usgs.gov/publication/70249990" }, { "@type": "PropertyValue", "propertyID": "USGS Publications Warehouse Internal ID", "value": 70249990 }, { "@type": "PropertyValue", "propertyID": "DOI", "value": "10.2144/btn-2023-0041", "url": "https://doi.org/10.2144/btn-2023-0041" } ], "journal": { "@type": "Periodical", "name": "BioTechniques", "volumeNumber": "75", "issueNumber": "6" }, "inLanguage": "en", "isPartOf": [ { "@type": "CreativeWorkSeries", "name": "BioTechniques" } ], "datePublished": "2023", "dateModified": "2023-12-21", "abstract": "A disadvantage of colorimetric detection in nucleic acid amplification assays is the possibility that\u00a0a colorblind individual may interpret colors differently than observers with full-color vision. Using an isothermal amplification assay, the ability of colorblind individuals to distinguish between positive and negative results for four dyes\u00a0was tested. Five individuals with self-reported colorblindness and four with full-color vision reported their observations of the color of the solution. Although colorblind individuals may accurately interpret assay results, they were often not accurate in reporting the color. Hydroxynaphthol blue was the most problematic dye, and both phenol red and SYBR\u2122 green were less troublesome. Consideration for colorblind individuals is warranted when developing an assay and training staff in its performance.", "description": "5 p.", "publisher": { "@type": "Organization", "name": "Future Science" }, "author": [ { "@type": "Person", "name": "Loyva, Kirstyn", "givenName": "Kirstyn", "familyName": "Loyva", "affiliation": [ { "@type": "Organization", "name": "U.S. Geological Survey, National Wildlife Health Center" } ] }, { "@type": "Person", "name": "Hofmeister, Erik K.", "givenName": "Erik K.", "familyName": "Hofmeister", "identifier": { "@type": "PropertyValue", "propertyID": "ORCID", "value": "0000-0002-2305-519X", "url": "https://orcid.org/0000-0002-2305-519X" }, "affiliation": [ { "@type": "Organization", "name": "National Wildlife Health Center", "url": "https://www.usgs.gov/centers/nwhc" } ] }, { "@type": "Person", "name": "Georgousi, Fiona", "givenName": "Fiona", "familyName": "Georgousi", "affiliation": [ { "@type": "Organization", "name": "U.S. Geological Survey, National Wildlife Health Center" } ] }, { "@type": "Person", "name": "Roderick, Constance", "givenName": "Constance", "familyName": "Roderick", "identifier": { "@type": "PropertyValue", "propertyID": "ORCID", "value": "0000-0001-8330-8024", "url": "https://orcid.org/0000-0001-8330-8024" }, "affiliation": [ { "@type": "Organization", "name": "National Wildlife Health Center", "url": "https://www.usgs.gov/centers/nwhc" } ] }, { "@type": "Person", "name": "Cole, Rebecca A. rcole@usgs.gov", "givenName": "Rebecca A.", "familyName": "Cole", "email": "rcole@usgs.gov", "identifier": { "@type": "PropertyValue", "propertyID": "ORCID", "value": "0000-0003-2923-1622", "url": "https://orcid.org/0000-0003-2923-1622" }, "affiliation": [ { "@type": "Organization", "name": "National Wildlife Health Center", "url": "https://www.usgs.gov/centers/nwhc" } ] } ], "funder": [ { "@type": "Organization", "name": "National Wildlife Health Center", "url": "https://www.usgs.gov/centers/nwhc" } ] }, "OpenAlex": { "_id": "https://openalex.org/w4388539201", "abstract_inverted_index": { "A": [ 0 ], "disadvantage": [ 1 ], "of": [ 2, 33, 61, 64 ], "colorimetric": [ 3 ], "detection": [ 4 ], "in": [ 5, 80, 114 ], "nucleic": [ 6 ], "acid": [ 7 ], "amplification": [ 8, 29 ], "assays": [ 9 ], "is": [ 10, 105 ], "the": [ 11, 31, 62, 65, 82, 87 ], "possibility": [ 12 ], "that": [ 13 ], "a": [ 14 ], "colorblind": [ 15, 34, 68, 103 ], "individual": [ 16 ], "may": [ 17, 70 ], "interpret": [ 18, 72 ], "colors": [ 19 ], "differently": [ 20 ], "than": [ 21 ], "observers": [ 22 ], "with": [ 23, 50, 55 ], "full-color": [ 24, 56 ], "vision.": [ 25 ], "Using": [ 26 ], "an": [ 27, 109 ], "isothermal": [ 28 ], "assay,": [ 30 ], "ability": [ 32 ], "individuals": [ 35, 49, 69, 104 ], "to": [ 36 ], "distinguish": [ 37 ], "between": [ 38 ], "positive": [ 39 ], "and": [ 40, 53, 91, 95, 111 ], "negative": [ 41 ], "results": [ 42 ], "for": [ 43, 102 ], "four": [ 44, 54 ], "dyes": [ 45 ], "was": [ 46, 86 ], "tested.": [ 47 ], "Five": [ 48 ], "self-reported": [ 51 ], "colorblindness": [ 52 ], "vision": [ 57 ], "reported": [ 58 ], "their": [ 59 ], "observations": [ 60 ], "color": [ 63 ], "solution.": [ 66 ], "Although": [ 67 ], "accurately": [ 71 ], "assay": [ 73, 110 ], "results,": [ 74 ], "they": [ 75 ], "were": [ 76, 98 ], "often": [ 77 ], "not": [ 78 ], "accurate": [ 79 ], "reporting": [ 81 ], "color.": [ 83 ], "Hydroxynaphthol": [ 84 ], "blue": [ 85 ], "most": [ 88 ], "problematic": [ 89 ], "dye,": [ 90 ], "both": [ 92 ], "phenol": [ 93 ], "red": [ 94 ], "SYBR\u2122": [ 96 ], "green": [ 97 ], "less": [ 99 ], "troublesome.": [ 100 ], "Consideration": [ 101 ], "warranted": [ 106 ], "when": [ 107 ], "developing": [ 108 ], "training": [ 112 ], "staff": [ 113 ], "its": [ 115 ], "performance.": [ 116 ] }, "apc_list": { "value": 1415, "currency": "USD", "value_usd": 1415, "provenance": "doaj" }, "apc_paid": { "value": 1415, "currency": "USD", "value_usd": 1415, "provenance": "doaj" }, "authorships": [ { "author_position": "first", "author": { "id": "https://openalex.org/A5093231959", "display_name": "Kirstyn Loyva", "orcid": null }, "institutions": [ { "id": "https://openalex.org/I4210131920", "display_name": "National Wildlife Health Center", "ror": "https://ror.org/038d10y34", "country_code": "US", "type": "healthcare", "lineage": [ "https://openalex.org/I1286329397", "https://openalex.org/I1335927249", "https://openalex.org/I4210131920" ] }, { "id": "https://openalex.org/I1286329397", "display_name": "United States Geological Survey", "ror": "https://ror.org/035a68863", "country_code": "US", "type": "government", "lineage": [ "https://openalex.org/I1286329397", "https://openalex.org/I1335927249" ] } ], "countries": [ "US" ], "is_corresponding": false, "raw_author_name": "Kirstyn Loyva", "raw_affiliation_strings": [ "U.S. Geological Survey, National Wildlife Health Center, Madison, WI53711, USA" ], "affiliations": [ { "raw_affiliation_string": "U.S. Geological Survey, National Wildlife Health Center, Madison, WI53711, USA", "institution_ids": [ "https://openalex.org/I4210131920", "https://openalex.org/I1286329397" ] } ] }, { "author_position": "middle", "author": { "id": "https://openalex.org/A5011676218", "display_name": "Erik K. Hofmeister", "orcid": "https://orcid.org/0000-0002-2305-519X" }, "institutions": [ { "id": "https://openalex.org/I4210131920", "display_name": "National Wildlife Health Center", "ror": "https://ror.org/038d10y34", "country_code": "US", "type": "healthcare", "lineage": [ "https://openalex.org/I1286329397", "https://openalex.org/I1335927249", "https://openalex.org/I4210131920" ] }, { "id": "https://openalex.org/I1286329397", "display_name": "United States Geological Survey", "ror": "https://ror.org/035a68863", "country_code": "US", "type": "government", "lineage": [ "https://openalex.org/I1286329397", "https://openalex.org/I1335927249" ] } ], "countries": [ "US" ], "is_corresponding": false, "raw_author_name": "Erik Hofmeister", "raw_affiliation_strings": [ "U.S. Geological Survey, National Wildlife Health Center, Madison, WI53711, USA" ], "affiliations": [ { "raw_affiliation_string": "U.S. Geological Survey, National Wildlife Health Center, Madison, WI53711, USA", "institution_ids": [ "https://openalex.org/I4210131920", "https://openalex.org/I1286329397" ] } ] }, { "author_position": "middle", "author": { "id": "https://openalex.org/A5091696685", "display_name": "Fiona Georgousi", "orcid": null }, "institutions": [ { "id": "https://openalex.org/I4210131920", "display_name": "National Wildlife Health Center", "ror": "https://ror.org/038d10y34", "country_code": "US", "type": "healthcare", "lineage": [ "https://openalex.org/I1286329397", "https://openalex.org/I1335927249", "https://openalex.org/I4210131920" ] }, { "id": "https://openalex.org/I1286329397", "display_name": "United States Geological Survey", "ror": "https://ror.org/035a68863", "country_code": "US", "type": "government", "lineage": [ "https://openalex.org/I1286329397", "https://openalex.org/I1335927249" ] } ], "countries": [ "US" ], "is_corresponding": false, "raw_author_name": "Fiona Georgousi", "raw_affiliation_strings": [ "U.S. Geological Survey, National Wildlife Health Center, Madison, WI53711, USA" ], "affiliations": [ { "raw_affiliation_string": "U.S. Geological Survey, National Wildlife Health Center, Madison, WI53711, USA", "institution_ids": [ "https://openalex.org/I4210131920", "https://openalex.org/I1286329397" ] } ] }, { "author_position": "middle", "author": { "id": "https://openalex.org/A5034704440", "display_name": "Constance L. Roderick", "orcid": "https://orcid.org/0000-0001-8330-8024" }, "institutions": [ { "id": "https://openalex.org/I4210131920", "display_name": "National Wildlife Health Center", "ror": "https://ror.org/038d10y34", "country_code": "US", "type": "healthcare", "lineage": [ "https://openalex.org/I1286329397", "https://openalex.org/I1335927249", "https://openalex.org/I4210131920" ] }, { "id": "https://openalex.org/I1286329397", "display_name": "United States Geological Survey", "ror": "https://ror.org/035a68863", "country_code": "US", "type": "government", "lineage": [ "https://openalex.org/I1286329397", "https://openalex.org/I1335927249" ] } ], "countries": [ "US" ], "is_corresponding": false, "raw_author_name": "Constance Roderick", "raw_affiliation_strings": [ "U.S. Geological Survey, National Wildlife Health Center, Madison, WI53711, USA" ], "affiliations": [ { "raw_affiliation_string": "U.S. Geological Survey, National Wildlife Health Center, Madison, WI53711, USA", "institution_ids": [ "https://openalex.org/I4210131920", "https://openalex.org/I1286329397" ] } ] }, { "author_position": "last", "author": { "id": "https://openalex.org/A5028915335", "display_name": "Rebecca A. Cole", "orcid": "https://orcid.org/0000-0003-2923-1622" }, "institutions": [ { "id": "https://openalex.org/I4210131920", "display_name": "National Wildlife Health Center", "ror": "https://ror.org/038d10y34", "country_code": "US", "type": "healthcare", "lineage": [ "https://openalex.org/I1286329397", "https://openalex.org/I1335927249", "https://openalex.org/I4210131920" ] }, { "id": "https://openalex.org/I1286329397", "display_name": "United States Geological Survey", "ror": "https://ror.org/035a68863", "country_code": "US", "type": "government", "lineage": [ "https://openalex.org/I1286329397", "https://openalex.org/I1335927249" ] } ], "countries": [ "US" ], "is_corresponding": false, "raw_author_name": "Rebecca Cole", "raw_affiliation_strings": [ "U.S. Geological Survey, National Wildlife Health Center, Madison, WI53711, USA" ], "affiliations": [ { "raw_affiliation_string": "U.S. Geological Survey, National Wildlife Health Center, Madison, WI53711, USA", "institution_ids": [ "https://openalex.org/I4210131920", "https://openalex.org/I1286329397" ] } ] } ], "best_oa_location": { "is_oa": true, "landing_page_url": "https://doi.org/10.2144/btn-2023-0041", "pdf_url": null, "source": { "id": "https://openalex.org/S79990748", "display_name": "BioTechniques", "issn_l": "0736-6205", "issn": [ "0736-6205", "1940-9818" ], "is_oa": true, "is_in_doaj": true, "is_core": true, "host_organization": "https://openalex.org/P4310320316", "host_organization_name": "Future Science Ltd", "host_organization_lineage": [ "https://openalex.org/P4310320316" ], "host_organization_lineage_names": [ "Future Science Ltd" ], "type": "journal" }, "license": "cc-by-nc-nd", "license_id": "https://openalex.org/licenses/cc-by-nc-nd", "version": "publishedVersion", "is_accepted": true, "is_published": true }, "biblio": { "volume": "75", "issue": "6", "first_page": "240", "last_page": "244" }, "citation_normalized_percentile": null, "cited_by_api_url": "https://api.openalex.org/works?filter=cites:W4388539201", "cited_by_count": 0, "cited_by_percentile_year": { "min": 0, "max": 74 }, "concepts": [ { "id": "https://openalex.org/C91881484", "wikidata": "https://www.wikidata.org/wiki/Q191807", "display_name": "Fluorescence", "level": 2, "score": 0.66978514 }, { "id": "https://openalex.org/C64299311", "wikidata": "https://www.wikidata.org/wiki/Q374535", "display_name": "Colorimetry", "level": 2, "score": 0.5622556 }, { "id": "https://openalex.org/C2777673361", "wikidata": "https://www.wikidata.org/wiki/Q5281228", "display_name": "Disadvantage", "level": 2, "score": 0.5280771 }, { "id": "https://openalex.org/C60635243", "wikidata": "https://www.wikidata.org/wiki/Q6675780", "display_name": "Loop-mediated isothermal amplification", "level": 3, "score": 0.45820832 }, { "id": "https://openalex.org/C24107716", "wikidata": "https://www.wikidata.org/wiki/Q123619", "display_name": "Nucleic acid", "level": 2, "score": 0.44515947 }, { "id": "https://openalex.org/C15744967", "wikidata": "https://www.wikidata.org/wiki/Q9418", "display_name": "Psychology", "level": 0, "score": 0.39695773 }, { "id": "https://openalex.org/C185592680", "wikidata": "https://www.wikidata.org/wiki/Q2329", "display_name": "Chemistry", "level": 0, "score": 0.39264292 }, { "id": "https://openalex.org/C43617362", "wikidata": "https://www.wikidata.org/wiki/Q170050", "display_name": "Chromatography", "level": 1, "score": 0.28595638 }, { "id": "https://openalex.org/C55493867", "wikidata": "https://www.wikidata.org/wiki/Q7094", "display_name": "Biochemistry", "level": 1, "score": 0.24178368 }, { "id": "https://openalex.org/C41008148", "wikidata": "https://www.wikidata.org/wiki/Q21198", "display_name": "Computer science", "level": 0, "score": 0.23032299 }, { "id": "https://openalex.org/C154945302", "wikidata": "https://www.wikidata.org/wiki/Q11660", "display_name": "Artificial intelligence", "level": 1, "score": 0.15817738 }, { "id": "https://openalex.org/C120665830", "wikidata": "https://www.wikidata.org/wiki/Q14620", "display_name": "Optics", "level": 1, "score": 0.13393441 }, { "id": "https://openalex.org/C552990157", "wikidata": "https://www.wikidata.org/wiki/Q7430", "display_name": "DNA", "level": 2, "score": 0.084239185 }, { "id": "https://openalex.org/C121332964", "wikidata": "https://www.wikidata.org/wiki/Q413", "display_name": "Physics", "level": 0, "score": 0.0 } ], "corresponding_author_ids": [], "corresponding_institution_ids": [], "countries_distinct_count": 1, "counts_by_year": [], "created_date": "2023-11-10", "datasets": [], "display_name": "Considerations for colorblind individuals on selecting colorimetric or fluorescent dye assay outcomes", "doi": "https://doi.org/10.2144/btn-2023-0041", "fwci": 0.0, "grants": [], "has_fulltext": false, "id": "https://openalex.org/W4388539201", "ids": { "openalex": "https://openalex.org/W4388539201", "doi": "https://doi.org/10.2144/btn-2023-0041", "pmid": "https://pubmed.ncbi.nlm.nih.gov/37942699" }, "indexed_in": [ "crossref", "pubmed" ], "institutions_distinct_count": 2, "is_paratext": false, "is_retracted": false, "keywords": [ { "id": "https://openalex.org/keywords/colorimetry", "display_name": "Colorimetry", "score": 0.5622556 }, { "id": "https://openalex.org/keywords/disadvantage", "display_name": "Disadvantage", "score": 0.5280771 }, { "id": "https://openalex.org/keywords/colorimetric-sensors", "display_name": "Colorimetric Sensors", "score": 0.524168 } ], "language": "en", "locations": [ { "is_oa": true, "landing_page_url": "https://doi.org/10.2144/btn-2023-0041", "pdf_url": null, "source": { "id": "https://openalex.org/S79990748", "display_name": "BioTechniques", "issn_l": "0736-6205", "issn": [ "0736-6205", "1940-9818" ], "is_oa": true, "is_in_doaj": true, "is_core": true, "host_organization": "https://openalex.org/P4310320316", "host_organization_name": "Future Science Ltd", "host_organization_lineage": [ "https://openalex.org/P4310320316" ], "host_organization_lineage_names": [ "Future Science Ltd" ], "type": "journal" }, "license": "cc-by-nc-nd", "license_id": "https://openalex.org/licenses/cc-by-nc-nd", "version": "publishedVersion", "is_accepted": true, "is_published": true }, { "is_oa": false, "landing_page_url": "https://pubmed.ncbi.nlm.nih.gov/37942699", "pdf_url": null, "source": { "id": "https://openalex.org/S4306525036", "display_name": "PubMed", "issn_l": null, "issn": null, "is_oa": false, "is_in_doaj": false, "is_core": false, "host_organization": "https://openalex.org/I1299303238", "host_organization_name": "National Institutes of Health", "host_organization_lineage": [ "https://openalex.org/I1299303238" ], "host_organization_lineage_names": [ "National Institutes of Health" ], "type": "repository" }, "license": null, "license_id": null, "version": null, "is_accepted": false, "is_published": false } ], "locations_count": 2, "mesh": [ { "descriptor_ui": "D003124", "descriptor_name": "Colorimetry", "qualifier_ui": "Q000379", "qualifier_name": "methods", "is_major_topic": false }, { "descriptor_ui": "D005456", "descriptor_name": "Fluorescent Dyes", "qualifier_ui": "", "qualifier_name": null, "is_major_topic": false }, { "descriptor_ui": "D006801", "descriptor_name": "Humans", "qualifier_ui": "", "qualifier_name": null, "is_major_topic": false }, { "descriptor_ui": "D021141", "descriptor_name": "Nucleic Acid Amplification Techniques", "qualifier_ui": "Q000379", "qualifier_name": "methods", "is_major_topic": false }, { "descriptor_ui": "D012680", "descriptor_name": "Sensitivity and Specificity", "qualifier_ui": "", "qualifier_name": null, "is_major_topic": false } ], "ngrams_url": "https://api.openalex.org/works/W4388539201/ngrams", "open_access": { "is_oa": true, "oa_status": "gold", "oa_url": "https://doi.org/10.2144/btn-2023-0041", "any_repository_has_fulltext": false }, "primary_location": { "is_oa": true, "landing_page_url": "https://doi.org/10.2144/btn-2023-0041", "pdf_url": null, "source": { "id": "https://openalex.org/S79990748", "display_name": "BioTechniques", "issn_l": "0736-6205", "issn": [ "0736-6205", "1940-9818" ], "is_oa": true, "is_in_doaj": true, "is_core": true, "host_organization": "https://openalex.org/P4310320316", "host_organization_name": "Future Science Ltd", "host_organization_lineage": [ "https://openalex.org/P4310320316" ], "host_organization_lineage_names": [ "Future Science Ltd" ], "type": "journal" }, "license": "cc-by-nc-nd", "license_id": "https://openalex.org/licenses/cc-by-nc-nd", "version": "publishedVersion", "is_accepted": true, "is_published": true }, "primary_topic": { "id": "https://openalex.org/T11393", "display_name": "Paper-Based Diagnostic Devices", "score": 0.9998, "subfield": { "id": "https://openalex.org/subfields/2204", "display_name": "Biomedical Engineering" }, "field": { "id": "https://openalex.org/fields/22", "display_name": "Engineering" }, "domain": { "id": "https://openalex.org/domains/3", "display_name": "Physical Sciences" } }, "publication_date": "2023-11-09", "publication_year": 2023, "referenced_works": [ "https://openalex.org/W1551643567", "https://openalex.org/W1979974453", "https://openalex.org/W2019875735", "https://openalex.org/W2020023709", "https://openalex.org/W2103785461", "https://openalex.org/W2105275554", "https://openalex.org/W2168174901", "https://openalex.org/W2549665412", "https://openalex.org/W2588534993", "https://openalex.org/W2767078106", "https://openalex.org/W3000172394", "https://openalex.org/W89741002" ], "referenced_works_count": 12, "related_works": [ "https://openalex.org/W2945075252", "https://openalex.org/W2390156094", "https://openalex.org/W2385043201", "https://openalex.org/W2378351399", "https://openalex.org/W2376505109", "https://openalex.org/W2347555292", "https://openalex.org/W227713497", "https://openalex.org/W2096415393", "https://openalex.org/W2009233031", "https://openalex.org/W2003087030" ], "sustainable_development_goals": [], "title": "Considerations for colorblind individuals on selecting colorimetric or fluorescent dye assay outcomes", "topics": [ { "id": "https://openalex.org/T11393", "display_name": "Paper-Based Diagnostic Devices", "score": 0.9998, "subfield": { "id": "https://openalex.org/subfields/2204", "display_name": "Biomedical Engineering" }, "field": { "id": "https://openalex.org/fields/22", "display_name": "Engineering" }, "domain": { "id": "https://openalex.org/domains/3", "display_name": "Physical Sciences" } }, { "id": "https://openalex.org/T11667", "display_name": "Breath Analysis Technology", "score": 0.987, "subfield": { "id": "https://openalex.org/subfields/2204", "display_name": "Biomedical Engineering" }, "field": { "id": "https://openalex.org/fields/22", "display_name": "Engineering" }, "domain": { "id": "https://openalex.org/domains/3", "display_name": "Physical Sciences" } }, { "id": "https://openalex.org/T10207", "display_name": "DNA Nanotechnology and Bioanalytical Applications", "score": 0.9814, "subfield": { "id": "https://openalex.org/subfields/1312", "display_name": "Molecular Biology" }, "field": { "id": "https://openalex.org/fields/13", "display_name": "Biochemistry, Genetics and Molecular Biology" }, "domain": { "id": "https://openalex.org/domains/1", "display_name": "Life Sciences" } } ], "type": "article", "type_crossref": "journal-article", "updated_date": "2024-08-11T22:05:32.127908", "versions": [] }
}