Item talk:Q273528

From geokb

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   "name": "Nutrient limitation of algae and macrophytes in streams: Integrating laboratory bioassays, field experiments, and field data",
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   "abstract": "Successful eutrophication control strategies need to address the limiting nutrient. We conducted a battery of laboratory and in situ nutrient-limitation tests with waters collected from 9 streams in an agricultural region of the upper Snake River basin, Idaho, USA. Laboratory tests used the green alga\u00a0Raphidocelis subcapitata, the macrophyte\u00a0Lemna minor\u00a0(duckweed) with native epiphytes, and in situ nutrient-limitation tests of periphyton were conducted with nutrient-diffusing substrates (NDS). In the duckweed/epiphyte test, P saturation occurred when concentrations reached about 100 \u03bcg/L. Chlorophyll\u00a0a\u00a0in epiphytic periphyton was stimulated at low P additions and by about 100 \u03bcg/L P, epiphytic periphyton chlorophyll\u00a0a\u00a0appeared to be P saturated. Both duckweed and epiphyte response patterns with total N were weaker but suggested a growth stimulation threshold for duckweed when total N concentrations exceeded about 300 \u03bcg/L and approached saturation at the highest N concentration tested, 1300 \u03bcg/L. Nutrient uptake by epiphytes and macrophytes removed up to 70 and 90% of the N and P, respectively. The green algae and the NDS nutrient-limitation test results were mostly congruent; N and P co-limitation was the most frequent result for both test series. Across all tests, when N:P molar ratios >30 (mass ratios >14), algae or macrophyte growth was P limited; N limitation was observed at N:P molar ratios up to 23 (mass ratios up to 10). A comparison of ambient periphyton chlorophyll\u00a0a\u00a0concentrations with chlorophyll\u00a0a\u00a0accrued on control artificial substrates in N-limited streams, suggests that total N concentrations associated with a periphyton chlorophyll\u00a0a\u00a0benchmark for desirable or undesirable conditions for recreation would be about 600 to 1000 \u03bcg/L total N, respectively. For P-limited streams, the corresponding benchmark concentrations were about 50 to 90 \u03bcg/L total P, respectively. Our approach of integrating controlled experiments and matched biomonitoring field surveys was cost effective and more informative than either approach alone.",
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