Item talk:Q273091
From geokb
{
"USGS Publications Warehouse": {
"@context": "https://schema.org",
"@type": "Article",
"additionalType": "Journal Article",
"name": "Establishment of a cell line with reticulo-endothelial characteristics from a rainbow trout spleen explant",
"identifier": [
{
"@type": "PropertyValue",
"propertyID": "USGS Publications Warehouse IndexID",
"value": "1014600",
"url": "https://pubs.usgs.gov/publication/1014600"
},
{
"@type": "PropertyValue",
"propertyID": "USGS Publications Warehouse Internal ID",
"value": 1014600
},
{
"@type": "PropertyValue",
"propertyID": "DOI",
"value": "10.3147/jsfp.25.165",
"url": "https://doi.org/10.3147/jsfp.25.165"
}
],
"journal": {
"@type": "Periodical",
"name": "Fish Pathology",
"volumeNumber": "25",
"issueNumber": "3"
},
"inLanguage": "en",
"isPartOf": [
{
"@type": "CreativeWorkSeries",
"name": "Fish Pathology"
}
],
"datePublished": "1990",
"dateModified": "2023-10-27",
"abstract": "A cell line from rainbow trout (Oncorhynchus mykiss) with phagocytic-like characteristics was established. First we found that it was necessary to inject the fish with antibiotics (penicillinstreptomycin) in order to clear tissue of bacterial contamination. Two days later, the spleen was excised and minced with scissors in MEM10. The large number of erythrocytes in the suspension were removed by low centrifugation, then the tissue fragments were incubated on tissue culture flasks at 18\u00b0C for 30 days with complete changes of media every other day.After 30 days in culture, primary explants from spleen were composed of three types of cells. The majority were large mononucleate, plecmorphic cells with thinly spread cytoplasm. These cells were trypsinized and subcultured. Another cell types was simple, round cells, somewhat similar to mature lymphocytes, while the third type appeared to be similar to fibroblastic cells.After 9 months with the culture in 17 to 20 passages, the predominate cell type was showing similarities to phagocytic cell. These cells had a marked reticular cytoplasm and grew individually without parallel orientations. By enzyme-specific stains, the cells were observed to have abundant acid phosphatase and \u03b2-glucuronidase activities of the cytoplasm. They were negative for peroxidase. The cells were also found to have highly adherent and phagocytic properties when incubated with latex particles.The morphological, enzymatic, and functional findings suggested the possibility that these cells in culture have some properties similar to the cells which are composed of reticulo-endothelial system.",
"description": "5 p.",
"publisher": {
"@type": "Organization",
"name": "Japanese Society of Fish Pathology"
},
"author": [
{
"@type": "Person",
"name": "Anderson, D. P.",
"givenName": "D. P.",
"familyName": "Anderson"
},
{
"@type": "Person",
"name": "Schill, W. B.",
"givenName": "W. B.",
"familyName": "Schill"
},
{
"@type": "Person",
"name": "Moritomo, T.",
"givenName": "T.",
"familyName": "Moritomo"
}
],
"funder": [
{
"@type": "Organization",
"name": "Leetown Science Center",
"url": "https://www.usgs.gov/centers/eesc"
}
]
},
"OpenAlex": {
"_id": "https://openalex.org/w1980535195",
"abstract_inverted_index": {
"A": [
0
],
"cell": [
1,
117,
151
],
"line": [
2
],
"from": [
3,
90
],
"rainbow": [
4
],
"trout": [
5
],
"(Oncorhynchus": [
6
],
"mykiss)": [
7
],
"with": [
8,
24,
44,
76,
106,
141,
209
],
"phagocytic-like": [
9
],
"characteristics": [
10
],
"was": [
11,
18,
40,
119,
153
],
"established.": [
12
],
"First": [
13
],
"we": [
14
],
"found": [
15,
199
],
"that": [
16,
220
],
"it": [
17
],
"necessary": [
19
],
"to": [
20,
29,
125,
133,
136,
146,
156,
179,
200,
229
],
"inject": [
21
],
"the": [
22,
38,
54,
62,
129,
142,
149,
175,
188,
218,
230
],
"fish": [
23
],
"antibiotics": [
25
],
"(penicillinstreptomycin)": [
26
],
"in": [
27,
46,
53,
86,
144,
223
],
"order": [
28
],
"clear": [
30
],
"tissue": [
31,
63,
68
],
"of": [
32,
51,
79,
94,
97,
187,
235
],
"bacterial": [
33
],
"contamination.": [
34
],
"Two": [
35
],
"days": [
36,
75,
85
],
"later,": [
37
],
"spleen": [
39,
91
],
"excised": [
41
],
"and": [
42,
114,
166,
184,
204,
214
],
"minced": [
43
],
"scissors": [
45
],
"MEM10.": [
47
],
"The": [
48,
99,
195
],
"large": [
49,
102
],
"number": [
50
],
"erythrocytes": [
52
],
"suspension": [
55
],
"were": [
56,
65,
92,
101,
112,
177,
191,
197
],
"removed": [
57
],
"by": [
58
],
"low": [
59
],
"centrifugation,": [
60
],
"then": [
61
],
"fragments": [
64
],
"incubated": [
66,
208
],
"on": [
67
],
"culture": [
69,
143,
224
],
"flasks": [
70
],
"at": [
71
],
"18\u00b0C": [
72
],
"for": [
73,
193
],
"30": [
74,
84
],
"complete": [
77
],
"changes": [
78
],
"media": [
80
],
"every": [
81
],
"other": [
82
],
"day.After": [
83
],
"culture,": [
87
],
"primary": [
88
],
"explants": [
89
],
"composed": [
93,
234
],
"three": [
95
],
"types": [
96,
118
],
"cells.": [
98
],
"majority": [
100
],
"mononucleate,": [
103
],
"plecmorphic": [
104
],
"cells": [
105,
111,
160,
176,
196,
222,
231
],
"thinly": [
107
],
"spread": [
108
],
"cytoplasm.": [
109,
189
],
"These": [
110,
159
],
"trypsinized": [
113
],
"subcultured.": [
115
],
"Another": [
116
],
"simple,": [
120
],
"round": [
121
],
"cells,": [
122
],
"somewhat": [
123
],
"similar": [
124,
135,
228
],
"mature": [
126
],
"lymphocytes,": [
127
],
"while": [
128
],
"third": [
130
],
"type": [
131,
152
],
"appeared": [
132
],
"be": [
134
],
"fibroblastic": [
137
],
"cells.After": [
138
],
"9": [
139
],
"months": [
140
],
"17": [
145
],
"20": [
147
],
"passages,": [
148
],
"predominate": [
150
],
"showing": [
154
],
"similarities": [
155
],
"phagocytic": [
157,
205
],
"cell.": [
158
],
"had": [
161
],
"a": [
162
],
"marked": [
163
],
"reticular": [
164
],
"cytoplasm": [
165
],
"grew": [
167
],
"individually": [
168
],
"without": [
169
],
"parallel": [
170
],
"orientations.": [
171
],
"By": [
172
],
"enzyme-specific": [
173
],
"stains,": [
174
],
"observed": [
178
],
"have": [
180,
201,
225
],
"abundant": [
181
],
"acid": [
182
],
"phosphatase": [
183
],
"\u03b2-glucuronidase": [
185
],
"activities": [
186
],
"They": [
190
],
"negative": [
192
],
"peroxidase.": [
194
],
"also": [
198
],
"highly": [
202
],
"adherent": [
203
],
"properties": [
206,
227
],
"when": [
207
],
"latex": [
210
],
"particles.The": [
211
],
"morphological,": [
212
],
"enzymatic,": [
213
],
"functional": [
215
],
"findings": [
216
],
"suggested": [
217
],
"possibility": [
219
],
"these": [
221
],
"some": [
226
],
"which": [
232
],
"are": [
233
],
"reticulo-endothelial": [
236
],
"system.": [
237
]
},
"apc_list": null,
"apc_paid": null,
"authorships": [
{
"author_position": "first",
"author": {
"id": "https://openalex.org/A5030337738",
"display_name": "Tadaaki Moritomo",
"orcid": null
},
"institutions": [],
"countries": [],
"is_corresponding": false,
"raw_author_name": "T. Moritomo",
"raw_affiliation_strings": [
"Laboratory of Fish Pathology, Department of Veterinary Science, College of Agriculture and Veterinary Medicine"
],
"affiliations": [
{
"raw_affiliation_string": "Laboratory of Fish Pathology, Department of Veterinary Science, College of Agriculture and Veterinary Medicine",
"institution_ids": []
}
]
},
{
"author_position": "middle",
"author": {
"id": "https://openalex.org/A5102962820",
"display_name": "D.P. Anderson",
"orcid": "https://orcid.org/0000-0003-4422-9520"
},
"institutions": [
{
"id": "https://openalex.org/I1343028186",
"display_name": "United States Fish and Wildlife Service",
"ror": "https://ror.org/04k7dar27",
"country_code": "US",
"type": "government",
"lineage": [
"https://openalex.org/I1335927249",
"https://openalex.org/I1343028186"
]
}
],
"countries": [
"US"
],
"is_corresponding": false,
"raw_author_name": "D.P. Anderson",
"raw_affiliation_strings": [
"U.S. Fish and Wildlife Service, National Fish Health Research Laboratory"
],
"affiliations": [
{
"raw_affiliation_string": "U.S. Fish and Wildlife Service, National Fish Health Research Laboratory",
"institution_ids": [
"https://openalex.org/I1343028186"
]
}
]
},
{
"author_position": "last",
"author": {
"id": "https://openalex.org/A5071420472",
"display_name": "William B. Schill",
"orcid": "https://orcid.org/0000-0002-9217-984X"
},
"institutions": [
{
"id": "https://openalex.org/I1343028186",
"display_name": "United States Fish and Wildlife Service",
"ror": "https://ror.org/04k7dar27",
"country_code": "US",
"type": "government",
"lineage": [
"https://openalex.org/I1335927249",
"https://openalex.org/I1343028186"
]
}
],
"countries": [
"US"
],
"is_corresponding": false,
"raw_author_name": "W.B. Schill",
"raw_affiliation_strings": [
"U.S. Fish and Wildlife Service, National Fish Health Research Laboratory"
],
"affiliations": [
{
"raw_affiliation_string": "U.S. Fish and Wildlife Service, National Fish Health Research Laboratory",
"institution_ids": [
"https://openalex.org/I1343028186"
]
}
]
}
],
"best_oa_location": {
"is_oa": true,
"landing_page_url": "https://doi.org/10.3147/jsfp.25.165",
"pdf_url": "https://www.jstage.jst.go.jp/article/jsfp1966/25/3/25_3_165/_pdf",
"source": {
"id": "https://openalex.org/S22730210",
"display_name": "Fish Pathology",
"issn_l": "0388-788X",
"issn": [
"0388-788X",
"1881-7335"
],
"is_oa": false,
"is_in_doaj": false,
"is_core": true,
"host_organization": "https://openalex.org/P4360969295",
"host_organization_name": "Japanese Society of Fish Pathology",
"host_organization_lineage": [
"https://openalex.org/P4360969295"
],
"host_organization_lineage_names": [
"Japanese Society of Fish Pathology"
],
"type": "journal"
},
"license": null,
"license_id": null,
"version": "publishedVersion",
"is_accepted": true,
"is_published": true
},
"biblio": {
"volume": "25",
"issue": "3",
"first_page": "165",
"last_page": "169"
},
"citation_normalized_percentile": {
"value": 0.740165,
"is_in_top_1_percent": false,
"is_in_top_10_percent": false
},
"cited_by_api_url": "https://api.openalex.org/works?filter=cites:W1980535195",
"cited_by_count": 16,
"cited_by_percentile_year": {
"min": 85,
"max": 86
},
"concepts": [
{
"id": "https://openalex.org/C86803240",
"wikidata": "https://www.wikidata.org/wiki/Q420",
"display_name": "Biology",
"level": 0,
"score": 0.82829076
},
{
"id": "https://openalex.org/C38240268",
"wikidata": "https://www.wikidata.org/wiki/Q17125118",
"display_name": "Trypsinization",
"level": 4,
"score": 0.7528484
},
{
"id": "https://openalex.org/C2994167347",
"wikidata": "https://www.wikidata.org/wiki/Q187986",
"display_name": "Rainbow trout",
"level": 3,
"score": 0.61683756
},
{
"id": "https://openalex.org/C190062978",
"wikidata": "https://www.wikidata.org/wiki/Q79899",
"display_name": "Cytoplasm",
"level": 2,
"score": 0.5968127
},
{
"id": "https://openalex.org/C2780931953",
"wikidata": "https://www.wikidata.org/wiki/Q9371",
"display_name": "Spleen",
"level": 2,
"score": 0.5963815
},
{
"id": "https://openalex.org/C157890192",
"wikidata": "https://www.wikidata.org/wiki/Q2561978",
"display_name": "Explant culture",
"level": 3,
"score": 0.5708134
},
{
"id": "https://openalex.org/C81885089",
"wikidata": "https://www.wikidata.org/wiki/Q189082",
"display_name": "Cell culture",
"level": 2,
"score": 0.5339972
},
{
"id": "https://openalex.org/C87863119",
"wikidata": "https://www.wikidata.org/wiki/Q842756",
"display_name": "Subculture (biology)",
"level": 2,
"score": 0.50231934
},
{
"id": "https://openalex.org/C189014844",
"wikidata": "https://www.wikidata.org/wiki/Q189118",
"display_name": "Cell type",
"level": 3,
"score": 0.47193
},
{
"id": "https://openalex.org/C90703135",
"wikidata": "https://www.wikidata.org/wiki/Q3287622",
"display_name": "Tissue culture",
"level": 3,
"score": 0.44124034
},
{
"id": "https://openalex.org/C153911025",
"wikidata": "https://www.wikidata.org/wiki/Q7202",
"display_name": "Molecular biology",
"level": 1,
"score": 0.41208485
},
{
"id": "https://openalex.org/C95444343",
"wikidata": "https://www.wikidata.org/wiki/Q7141",
"display_name": "Cell biology",
"level": 1,
"score": 0.35490784
},
{
"id": "https://openalex.org/C1491633281",
"wikidata": "https://www.wikidata.org/wiki/Q7868",
"display_name": "Cell",
"level": 2,
"score": 0.3522412
},
{
"id": "https://openalex.org/C89423630",
"wikidata": "https://www.wikidata.org/wiki/Q7193",
"display_name": "Microbiology",
"level": 1,
"score": 0.29491234
},
{
"id": "https://openalex.org/C202751555",
"wikidata": "https://www.wikidata.org/wiki/Q221681",
"display_name": "In vitro",
"level": 2,
"score": 0.2359761
},
{
"id": "https://openalex.org/C203014093",
"wikidata": "https://www.wikidata.org/wiki/Q101929",
"display_name": "Immunology",
"level": 1,
"score": 0.23424652
},
{
"id": "https://openalex.org/C2780340462",
"wikidata": "https://www.wikidata.org/wiki/Q127450",
"display_name": "Trypsin",
"level": 3,
"score": 0.19052717
},
{
"id": "https://openalex.org/C55493867",
"wikidata": "https://www.wikidata.org/wiki/Q7094",
"display_name": "Biochemistry",
"level": 1,
"score": 0.18862432
},
{
"id": "https://openalex.org/C181199279",
"wikidata": "https://www.wikidata.org/wiki/Q8047",
"display_name": "Enzyme",
"level": 2,
"score": 0.17955765
},
{
"id": "https://openalex.org/C2909208804",
"wikidata": "https://www.wikidata.org/wiki/Q127282",
"display_name": "Fish <Actinopterygii>",
"level": 2,
"score": 0.12459427
},
{
"id": "https://openalex.org/C54355233",
"wikidata": "https://www.wikidata.org/wiki/Q7162",
"display_name": "Genetics",
"level": 1,
"score": 0.0
},
{
"id": "https://openalex.org/C505870484",
"wikidata": "https://www.wikidata.org/wiki/Q180538",
"display_name": "Fishery",
"level": 1,
"score": 0.0
}
],
"corresponding_author_ids": [],
"corresponding_institution_ids": [],
"countries_distinct_count": 1,
"counts_by_year": [
{
"year": 2023,
"cited_by_count": 2
},
{
"year": 2022,
"cited_by_count": 1
},
{
"year": 2017,
"cited_by_count": 1
},
{
"year": 2016,
"cited_by_count": 1
}
],
"created_date": "2016-06-24",
"datasets": [],
"display_name": "Establishment of a Cell Line with Reticulo-endothelial Characteristics from a Rainbow Trout Spleen Explant",
"doi": "https://doi.org/10.3147/jsfp.25.165",
"fulltext_origin": "pdf",
"fwci": 0.153,
"grants": [],
"has_fulltext": true,
"id": "https://openalex.org/W1980535195",
"ids": {
"openalex": "https://openalex.org/W1980535195",
"doi": "https://doi.org/10.3147/jsfp.25.165",
"mag": "1980535195"
},
"indexed_in": [
"crossref"
],
"institutions_distinct_count": 1,
"is_paratext": false,
"is_retracted": false,
"keywords": [
{
"id": "https://openalex.org/keywords/trypsinization",
"display_name": "Trypsinization",
"score": 0.7528484
},
{
"id": "https://openalex.org/keywords/subculture",
"display_name": "Subculture (biology)",
"score": 0.50231934
},
{
"id": "https://openalex.org/keywords/fish-immunology",
"display_name": "Fish Immunology",
"score": 0.498847
},
{
"id": "https://openalex.org/keywords/cell-type",
"display_name": "Cell type",
"score": 0.47193
}
],
"language": "en",
"locations": [
{
"is_oa": true,
"landing_page_url": "https://doi.org/10.3147/jsfp.25.165",
"pdf_url": "https://www.jstage.jst.go.jp/article/jsfp1966/25/3/25_3_165/_pdf",
"source": {
"id": "https://openalex.org/S22730210",
"display_name": "Fish Pathology",
"issn_l": "0388-788X",
"issn": [
"0388-788X",
"1881-7335"
],
"is_oa": false,
"is_in_doaj": false,
"is_core": true,
"host_organization": "https://openalex.org/P4360969295",
"host_organization_name": "Japanese Society of Fish Pathology",
"host_organization_lineage": [
"https://openalex.org/P4360969295"
],
"host_organization_lineage_names": [
"Japanese Society of Fish Pathology"
],
"type": "journal"
},
"license": null,
"license_id": null,
"version": "publishedVersion",
"is_accepted": true,
"is_published": true
}
],
"locations_count": 1,
"mesh": [],
"ngrams_url": "https://api.openalex.org/works/W1980535195/ngrams",
"open_access": {
"is_oa": true,
"oa_status": "bronze",
"oa_url": "https://www.jstage.jst.go.jp/article/jsfp1966/25/3/25_3_165/_pdf",
"any_repository_has_fulltext": false
},
"primary_location": {
"is_oa": true,
"landing_page_url": "https://doi.org/10.3147/jsfp.25.165",
"pdf_url": "https://www.jstage.jst.go.jp/article/jsfp1966/25/3/25_3_165/_pdf",
"source": {
"id": "https://openalex.org/S22730210",
"display_name": "Fish Pathology",
"issn_l": "0388-788X",
"issn": [
"0388-788X",
"1881-7335"
],
"is_oa": false,
"is_in_doaj": false,
"is_core": true,
"host_organization": "https://openalex.org/P4360969295",
"host_organization_name": "Japanese Society of Fish Pathology",
"host_organization_lineage": [
"https://openalex.org/P4360969295"
],
"host_organization_lineage_names": [
"Japanese Society of Fish Pathology"
],
"type": "journal"
},
"license": null,
"license_id": null,
"version": "publishedVersion",
"is_accepted": true,
"is_published": true
},
"primary_topic": {
"id": "https://openalex.org/T10506",
"display_name": "Immunological Responses in Aquatic Organisms",
"score": 0.9994,
"subfield": {
"id": "https://openalex.org/subfields/2403",
"display_name": "Immunology"
},
"field": {
"id": "https://openalex.org/fields/24",
"display_name": "Immunology and Microbiology"
},
"domain": {
"id": "https://openalex.org/domains/1",
"display_name": "Life Sciences"
}
},
"publication_date": "1990-01-01",
"publication_year": 1990,
"referenced_works": [
"https://openalex.org/W1556654481",
"https://openalex.org/W1729011202",
"https://openalex.org/W1987149214",
"https://openalex.org/W2047455844",
"https://openalex.org/W2072443596",
"https://openalex.org/W2087847435",
"https://openalex.org/W2329211716",
"https://openalex.org/W36048989"
],
"referenced_works_count": 8,
"related_works": [
"https://openalex.org/W4249412307",
"https://openalex.org/W4200359069",
"https://openalex.org/W2391514006",
"https://openalex.org/W2388058729",
"https://openalex.org/W2382217790",
"https://openalex.org/W2360744735",
"https://openalex.org/W2349254943",
"https://openalex.org/W2062867338",
"https://openalex.org/W1997912285",
"https://openalex.org/W135003083"
],
"sustainable_development_goals": [
{
"display_name": "Life below water",
"score": 0.81,
"id": "https://metadata.un.org/sdg/14"
}
],
"title": "Establishment of a Cell Line with Reticulo-endothelial Characteristics from a Rainbow Trout Spleen Explant",
"topics": [
{
"id": "https://openalex.org/T10506",
"display_name": "Immunological Responses in Aquatic Organisms",
"score": 0.9994,
"subfield": {
"id": "https://openalex.org/subfields/2403",
"display_name": "Immunology"
},
"field": {
"id": "https://openalex.org/fields/24",
"display_name": "Immunology and Microbiology"
},
"domain": {
"id": "https://openalex.org/domains/1",
"display_name": "Life Sciences"
}
},
{
"id": "https://openalex.org/T11403",
"display_name": "Invertebrate Immunity and Host Defense Mechanisms",
"score": 0.9716,
"subfield": {
"id": "https://openalex.org/subfields/2403",
"display_name": "Immunology"
},
"field": {
"id": "https://openalex.org/fields/24",
"display_name": "Immunology and Microbiology"
},
"domain": {
"id": "https://openalex.org/domains/1",
"display_name": "Life Sciences"
}
},
{
"id": "https://openalex.org/T11685",
"display_name": "Zebrafish as a Model Organism for Multidisciplinary Research",
"score": 0.9609,
"subfield": {
"id": "https://openalex.org/subfields/1307",
"display_name": "Cell Biology"
},
"field": {
"id": "https://openalex.org/fields/13",
"display_name": "Biochemistry, Genetics and Molecular Biology"
},
"domain": {
"id": "https://openalex.org/domains/1",
"display_name": "Life Sciences"
}
}
],
"type": "article",
"type_crossref": "journal-article",
"updated_date": "2024-08-13T02:35:36.188473",
"versions": []
}
}
