Item talk:Q266401
From geokb
{
"USGS Publications Warehouse": { "@context": "https://schema.org", "@type": "Article", "additionalType": "Journal Article", "name": "Factors affecting the efficient transformation of Colletotrichum species", "identifier": [ { "@type": "PropertyValue", "propertyID": "USGS Publications Warehouse IndexID", "value": "70180425", "url": "https://pubs.usgs.gov/publication/70180425" }, { "@type": "PropertyValue", "propertyID": "USGS Publications Warehouse Internal ID", "value": 70180425 }, { "@type": "PropertyValue", "propertyID": "DOI", "value": "10.1006/emyc.1994.1023", "url": "https://doi.org/10.1006/emyc.1994.1023" } ], "journal": { "@type": "Periodical", "name": "Experimental Mycology ", "volumeNumber": "18", "issueNumber": "3" }, "inLanguage": "en", "isPartOf": [ { "@type": "CreativeWorkSeries", "name": "Experimental Mycology " } ], "datePublished": "1994", "dateModified": "2017-01-30", "abstract": "Factors affecting the efficient transformation of Colletotrichum species. Experimental Mycology, 18, 230-246. Twelve isolates representing four species of Colletotrichum were transformed either by enhanced protoplast, restriction enzyme-mediated integration (REMI), or electroporation-mediated protocols. The enhanced protoplast transformation protocol resulted in 100- and 50-fold increases in the transformation efficiencies of Colletotrichum lindemuthianum and C. magna , respectively. REMI transformation involved the use of Hin dIII and vector DNA linearized with HindIII to increase the number of integration events and potential gene disruptions in the fungal genome. Combining the enhanced protoplast and the REMI protocols resulted in a 22-fold increase in the number of hygromycin/nystatin-resistant mutants in C. lindemuthianum . Electroporation-mediated transformation was performed on mycelial fragments and spores of four Colletotrichum species, resulting in efficiencies of up to 1000 transformants/\u03bcg DNA. The pHA1.3 vector which confers hygromycin resistance contains telomeric sequences from Fusarium oxysporum , transforms by autonomous replication and genomic integration, and was essential for elevated transformation efficiencies of 100 to 10,000 transformants/\u03bcg DNA. Modifications of pHA1.3 occurred during bacterial amplification and post fungal transformation resulting in plasmids capable of significantly elevated transformation efficiencies in C. lindemuthianum.", "description": "17 p. ", "publisher": { "@type": "Organization", "name": "Elsevier" }, "author": [ { "@type": "Person", "name": "Redman, Regina S.", "givenName": "Regina S.", "familyName": "Redman", "identifier": { "@type": "PropertyValue", "propertyID": "ORCID", "value": "0000-0001-5108-7570", "url": "https://orcid.org/0000-0001-5108-7570" }, "affiliation": [ { "@type": "Organization", "name": "Western Fisheries Research Center", "url": "https://www.usgs.gov/centers/western-fisheries-research-center" } ] }, { "@type": "Person", "name": "Rodriguez, Rusty J.", "givenName": "Rusty J.", "familyName": "Rodriguez" } ], "funder": [ { "@type": "Organization", "name": "Western Fisheries Research Center", "url": "https://www.usgs.gov/centers/western-fisheries-research-center" } ] }, "OpenAlex": { "_id": "https://openalex.org/w1997843053", "abstract_inverted_index": { "Redman,": [ 0 ], "R.": [ 1, 5 ], "S.,": [ 2 ], "and": [ 3, 48, 58, 71, 84, 96, 122, 155, 158, 178 ], "Rodriguez,": [ 4 ], "J.": [ 6 ], "1994.": [ 7 ], "Factors": [ 8 ], "affecting": [ 9 ], "the": [ 10, 52, 66, 79, 89, 93, 97, 106 ], "efficient": [ 11 ], "transformation": [ 12, 43, 53, 64, 116, 163, 181, 189 ], "of": [ 13, 25, 55, 68, 81, 108, 124, 131, 165, 172, 186 ], "Colletotrichum": [ 14, 26, 56, 126 ], "species.": [ 15 ], "Experimental": [ 16 ], "Mycology,": [ 17 ], "18,": [ 18 ], "230-246.": [ 19 ], "Twelve": [ 20 ], "isolates": [ 21 ], "representing": [ 22 ], "four": [ 23, 125 ], "species": [ 24 ], "were": [ 27 ], "transformed": [ 28 ], "either": [ 29 ], "by": [ 30, 152 ], "enhanced": [ 31, 41, 94 ], "protoplast,": [ 32 ], "restriction": [ 33 ], "enzyme-mediated": [ 34 ], "integration": [ 35, 82 ], "(REMI),": [ 36 ], "or": [ 37 ], "electroporation-mediated": [ 38 ], "protocols.": [ 39 ], "The": [ 40, 137 ], "protoplast": [ 42, 95 ], "protocol": [ 44 ], "resulted": [ 45, 100 ], "in": [ 46, 51, 88, 101, 105, 111, 129, 183, 191 ], "100-": [ 47 ], "50-fold": [ 49 ], "increases": [ 50 ], "efficiencies": [ 54, 130, 164, 190 ], "lindemuthianum": [ 57, 113 ], "C.": [ 59, 112, 192 ], "magna": [ 60 ], ",": [ 61, 150 ], "respectively.": [ 62 ], "REMI": [ 63, 98 ], "involved": [ 65 ], "use": [ 67 ], "Hin": [ 69 ], "dIII": [ 70 ], "vector": [ 72, 139 ], "DNA": [ 73 ], "linearized": [ 74 ], "with": [ 75 ], "HindIII": [ 76 ], "to": [ 77, 133, 167 ], "increase": [ 78, 104 ], "number": [ 80, 107 ], "events": [ 83 ], "potential": [ 85 ], "gene": [ 86 ], "disruptions": [ 87 ], "fungal": [ 90, 180 ], "genome.": [ 91 ], "Combining": [ 92 ], "protocols": [ 99 ], "a": [ 102 ], "22-fold": [ 103 ], "hygromycin/nystatin-resistant": [ 109 ], "mutants": [ 110 ], ".": [ 114 ], "Electroporation-mediated": [ 115 ], "was": [ 117, 159 ], "performed": [ 118 ], "on": [ 119 ], "mycelial": [ 120 ], "fragments": [ 121 ], "spores": [ 123 ], "species,": [ 127 ], "resulting": [ 128, 182 ], "up": [ 132 ], "1000": [ 134 ], "transformants/\u03bcg": [ 135, 169 ], "DNA.": [ 136, 170 ], "pHA1.3": [ 138, 173 ], "which": [ 140 ], "confers": [ 141 ], "hygromycin": [ 142 ], "resistance": [ 143 ], "contains": [ 144 ], "telomeric": [ 145 ], "sequences": [ 146 ], "from": [ 147 ], "Fusarium": [ 148 ], "oxysporum": [ 149 ], "transforms": [ 151 ], "autonomous": [ 153 ], "replication": [ 154 ], "genomic": [ 156 ], "integration,": [ 157 ], "essential": [ 160 ], "for": [ 161 ], "elevated": [ 162, 188 ], "100": [ 166 ], "10,000": [ 168 ], "Modifications": [ 171 ], "occurred": [ 174 ], "during": [ 175 ], "bacterial": [ 176 ], "amplification": [ 177 ], "post": [ 179 ], "plasmids": [ 184 ], "capable": [ 185 ], "significantly": [ 187 ], "lindemuthianum.": [ 193 ] }, "apc_list": null, "apc_paid": null, "authorships": [ { "author_position": "first", "author": { "id": "https://openalex.org/A5064991754", "display_name": "Regina S. Redman", "orcid": null }, "institutions": [ { "id": "https://openalex.org/I103635307", "display_name": "University of California, Riverside", "ror": "https://ror.org/03nawhv43", "country_code": "US", "type": "education", "lineage": [ "https://openalex.org/I103635307" ] } ], "countries": [ "US" ], "is_corresponding": false, "raw_author_name": "Regina S. Redman", "raw_affiliation_strings": [ "Department of Plant Pathology, University of California, Riverside, California 92521", "National Biological Survey, Seattle, Washington 98115" ], "affiliations": [ { "raw_affiliation_string": "National Biological Survey, Seattle, Washington 98115", "institution_ids": [] }, { "raw_affiliation_string": "Department of Plant Pathology, University of California, Riverside, California 92521", "institution_ids": [ "https://openalex.org/I103635307" ] } ] }, { "author_position": "last", "author": { "id": "https://openalex.org/A5066283628", "display_name": "Rusty J. Rodriguez", "orcid": null }, "institutions": [ { "id": "https://openalex.org/I103635307", "display_name": "University of California, Riverside", "ror": "https://ror.org/03nawhv43", "country_code": "US", "type": "education", "lineage": [ "https://openalex.org/I103635307" ] } ], "countries": [ "US" ], "is_corresponding": false, "raw_author_name": "Rusty J. Rodriguez", "raw_affiliation_strings": [ "Department of Plant Pathology, University of California, Riverside, California 92521", "National Biological Survey, Seattle, Washington 98115" ], "affiliations": [ { "raw_affiliation_string": "National Biological Survey, Seattle, Washington 98115", "institution_ids": [] }, { "raw_affiliation_string": "Department of Plant Pathology, University of California, Riverside, California 92521", "institution_ids": [ "https://openalex.org/I103635307" ] } ] } ], "best_oa_location": null, "biblio": { "volume": "18", "issue": "3", "first_page": "230", "last_page": "246" }, "citation_normalized_percentile": { "value": 0.838436, "is_in_top_1_percent": false, "is_in_top_10_percent": false }, "cited_by_api_url": "https://api.openalex.org/works?filter=cites:W1997843053", "cited_by_count": 50, "cited_by_percentile_year": { "min": 93, "max": 94 }, "concepts": [ { "id": "https://openalex.org/C2778353340", "wikidata": "https://www.wikidata.org/wiki/Q2853020", "display_name": "Colletotrichum lindemuthianum", "level": 3, "score": 0.88184804 }, { "id": "https://openalex.org/C86803240", "wikidata": "https://www.wikidata.org/wiki/Q420", "display_name": "Biology", "level": 0, "score": 0.84664226 }, { "id": "https://openalex.org/C204241405", "wikidata": "https://www.wikidata.org/wiki/Q461499", "display_name": "Transformation (genetics)", "level": 3, "score": 0.8004329 }, { "id": "https://openalex.org/C173749725", "wikidata": "https://www.wikidata.org/wiki/Q1126500", "display_name": "Protoplast", "level": 2, "score": 0.74072087 }, { "id": "https://openalex.org/C2780058085", "wikidata": "https://www.wikidata.org/wiki/Q418735", "display_name": "Hygromycin B", "level": 4, "score": 0.71307385 }, { "id": "https://openalex.org/C178050479", "wikidata": "https://www.wikidata.org/wiki/Q7834084", "display_name": "Transformation efficiency", "level": 5, "score": 0.6571642 }, { "id": "https://openalex.org/C186852380", "wikidata": "https://www.wikidata.org/wiki/Q1142521", "display_name": "Electroporation", "level": 3, "score": 0.6289535 }, { "id": "https://openalex.org/C22744801", "wikidata": "https://www.wikidata.org/wiki/Q172778", "display_name": "Plasmid", "level": 3, "score": 0.5899342 }, { "id": "https://openalex.org/C2779542283", "wikidata": "https://www.wikidata.org/wiki/Q10457032", "display_name": "Colletotrichum", "level": 2, "score": 0.5542372 }, { "id": "https://openalex.org/C89423630", "wikidata": "https://www.wikidata.org/wiki/Q7193", "display_name": "Microbiology", "level": 1, "score": 0.5459652 }, { "id": "https://openalex.org/C2779430813", "wikidata": "https://www.wikidata.org/wiki/Q139958", "display_name": "Fusarium oxysporum", "level": 2, "score": 0.48288727 }, { "id": "https://openalex.org/C54355233", "wikidata": "https://www.wikidata.org/wiki/Q7162", "display_name": "Genetics", "level": 1, "score": 0.29661986 }, { "id": "https://openalex.org/C552990157", "wikidata": "https://www.wikidata.org/wiki/Q7430", "display_name": "DNA", "level": 2, "score": 0.26937592 }, { "id": "https://openalex.org/C59822182", "wikidata": "https://www.wikidata.org/wiki/Q441", "display_name": "Botany", "level": 1, "score": 0.21461025 }, { "id": "https://openalex.org/C104317684", "wikidata": "https://www.wikidata.org/wiki/Q7187", "display_name": "Gene", "level": 2, "score": 0.19516557 }, { "id": "https://openalex.org/C2779656422", "wikidata": "https://www.wikidata.org/wiki/Q2700446", "display_name": "Agrobacterium", "level": 4, "score": 0.115036696 }, { "id": "https://openalex.org/C197321923", "wikidata": "https://www.wikidata.org/wiki/Q4886", "display_name": "Cultivar", "level": 2, "score": 0.0 } ], "corresponding_author_ids": [], "corresponding_institution_ids": [], "countries_distinct_count": 1, "counts_by_year": [ { "year": 2024, "cited_by_count": 1 }, { "year": 2023, "cited_by_count": 2 }, { "year": 2022, "cited_by_count": 1 }, { "year": 2020, "cited_by_count": 1 }, { "year": 2019, "cited_by_count": 1 }, { "year": 2016, "cited_by_count": 1 }, { "year": 2014, "cited_by_count": 2 }, { "year": 2013, "cited_by_count": 2 } ], "created_date": "2016-06-24", "datasets": [], "display_name": "Factors Affecting the Efficient Transformation of Colletotrichum Species", "doi": "https://doi.org/10.1006/emyc.1994.1023", "fwci": 0.475, "grants": [], "has_fulltext": false, "id": "https://openalex.org/W1997843053", "ids": { "openalex": "https://openalex.org/W1997843053", "doi": "https://doi.org/10.1006/emyc.1994.1023", "mag": "1997843053" }, "indexed_in": [ "crossref" ], "institutions_distinct_count": 1, "is_paratext": false, "is_retracted": false, "keywords": [ { "id": "https://openalex.org/keywords/colletotrichum-lindemuthianum", "display_name": "Colletotrichum lindemuthianum", "score": 0.88184804 }, { "id": "https://openalex.org/keywords/protoplast", "display_name": "Protoplast", "score": 0.74072087 }, { "id": "https://openalex.org/keywords/hygromycin-b", "display_name": "Hygromycin B", "score": 0.71307385 }, { "id": "https://openalex.org/keywords/transformation-efficiency", "display_name": "Transformation efficiency", "score": 0.6571642 }, { "id": "https://openalex.org/keywords/colletotrichum", "display_name": "Colletotrichum", "score": 0.5542372 }, { "id": "https://openalex.org/keywords/species-recognition", "display_name": "Species Recognition", "score": 0.446214 } ], "language": "en", "locations": [ { "is_oa": false, "landing_page_url": "https://doi.org/10.1006/emyc.1994.1023", "pdf_url": null, "source": { "id": "https://openalex.org/S166883710", "display_name": "Experimental Mycology", "issn_l": "0147-5975", "issn": [ "0147-5975", "1878-4399" ], "is_oa": false, "is_in_doaj": false, "is_core": false, "host_organization": "https://openalex.org/P4310319955", "host_organization_name": "Academic Press", "host_organization_lineage": [ "https://openalex.org/P4310320990", "https://openalex.org/P4310319955" ], "host_organization_lineage_names": [ "Elsevier BV", "Academic Press" ], "type": "journal" }, "license": null, "license_id": null, "version": null, "is_accepted": false, "is_published": false } ], "locations_count": 1, "mesh": [], "ngrams_url": "https://api.openalex.org/works/W1997843053/ngrams", "open_access": { "is_oa": false, "oa_status": "closed", "oa_url": null, "any_repository_has_fulltext": false }, "primary_location": { "is_oa": false, "landing_page_url": "https://doi.org/10.1006/emyc.1994.1023", "pdf_url": null, "source": { "id": "https://openalex.org/S166883710", "display_name": "Experimental Mycology", "issn_l": "0147-5975", "issn": [ "0147-5975", "1878-4399" ], "is_oa": false, "is_in_doaj": false, "is_core": false, "host_organization": "https://openalex.org/P4310319955", "host_organization_name": "Academic Press", "host_organization_lineage": [ "https://openalex.org/P4310320990", "https://openalex.org/P4310319955" ], "host_organization_lineage_names": [ "Elsevier BV", "Academic Press" ], "type": "journal" }, "license": null, "license_id": null, "version": null, "is_accepted": false, "is_published": false }, "primary_topic": { "id": "https://openalex.org/T10825", "display_name": "Diversity and Evolution of Fungal Pathogens", "score": 0.9995, "subfield": { "id": "https://openalex.org/subfields/1307", "display_name": "Cell Biology" }, "field": { "id": "https://openalex.org/fields/13", "display_name": "Biochemistry, Genetics and Molecular Biology" }, "domain": { "id": "https://openalex.org/domains/1", "display_name": "Life Sciences" } }, "publication_date": "1994-09-01", "publication_year": 1994, "referenced_works": [], "referenced_works_count": 0, "related_works": [ "https://openalex.org/W4236312592", "https://openalex.org/W2387694660", "https://openalex.org/W2318165898", "https://openalex.org/W2142388360", "https://openalex.org/W2069228308", "https://openalex.org/W2033635923", "https://openalex.org/W2017112672", "https://openalex.org/W1997843053", "https://openalex.org/W1969523487", "https://openalex.org/W1963591278" ], "sustainable_development_goals": [ { "id": "https://metadata.un.org/sdg/15", "score": 0.58, "display_name": "Life on land" } ], "title": "Factors Affecting the Efficient Transformation of Colletotrichum Species", "topics": [ { "id": "https://openalex.org/T10825", "display_name": "Diversity and Evolution of Fungal Pathogens", "score": 0.9995, "subfield": { "id": "https://openalex.org/subfields/1307", "display_name": "Cell Biology" }, "field": { "id": "https://openalex.org/fields/13", "display_name": "Biochemistry, Genetics and Molecular Biology" }, "domain": { "id": "https://openalex.org/domains/1", "display_name": "Life Sciences" } }, { "id": "https://openalex.org/T10520", "display_name": "Mycotoxins and Their Impact", "score": 0.9901, "subfield": { "id": "https://openalex.org/subfields/1110", "display_name": "Plant Science" }, "field": { "id": "https://openalex.org/fields/11", "display_name": "Agricultural and Biological Sciences" }, "domain": { "id": "https://openalex.org/domains/1", "display_name": "Life Sciences" } }, { "id": "https://openalex.org/T12668", "display_name": "Diversity and Systematics of Yeasts", "score": 0.9865, "subfield": { "id": "https://openalex.org/subfields/1312", "display_name": "Molecular Biology" }, "field": { "id": "https://openalex.org/fields/13", "display_name": "Biochemistry, Genetics and Molecular Biology" }, "domain": { "id": "https://openalex.org/domains/1", "display_name": "Life Sciences" } } ], "type": "article", "type_crossref": "journal-article", "updated_date": "2024-08-11T15:16:40.330762", "versions": [] }
}