Item talk:Q260149
From geokb
{
"USGS Publications Warehouse": { "@context": "https://schema.org", "@type": "Article", "additionalType": "Journal Article", "name": "Cryptosporidium incidence and surface water in\ufb02uence of groundwater supplying public water systems in Minnesota, USA", "identifier": [ { "@type": "PropertyValue", "propertyID": "USGS Publications Warehouse IndexID", "value": "70202720", "url": "https://pubs.usgs.gov/publication/70202720" }, { "@type": "PropertyValue", "propertyID": "USGS Publications Warehouse Internal ID", "value": 70202720 }, { "@type": "PropertyValue", "propertyID": "DOI", "value": "10.1021/acs.est.8b05446", "url": "https://doi.org/10.1021/acs.est.8b05446" } ], "journal": { "@type": "Periodical", "name": "Environmental Science & Technology", "volumeNumber": "23", "issueNumber": "7" }, "inLanguage": "en", "isPartOf": [ { "@type": "CreativeWorkSeries", "name": "Environmental Science & Technology" } ], "datePublished": "2019", "dateModified": "2019-06-18", "abstract": "Regulations for public water systems (PWS) in the U.S. consider Cryptosporidium a microbial contaminant of surface water supplies. Ground- water is assumed free of Cryptosporidium unless surface water is entering supply wells. We determined the incidence of Cryptosporidium in PWS wells varying in surface water in\ufb02uence. Community and noncommunity PWS wells (n = 145) were sampled (n = 964) and analyzed for Cryptosporidium by qPCR and immuno\ufb02uorescence assay (IFA). Surface water in\ufb02uence was assessed by stable isotopes and the expert judgment of hydrogeologists using site-speci\ufb01c data. Fifty-eight wells (40%) and 107 samples (11%) were Cryptosporidium- positive by qPCR, and of these samples 67 were positive by IFA. Cryptosporidium concentrations measured by qPCR and IFA were signi\ufb01cantly\ncorrelated (p < 0.001). Cryptosporidium incidence was not associated with surface water in\ufb02uence as assessed by stable isotopes or expert judgment. We successfully sequenced 45 of the 107 positive samples to identify species, including C. parvum (41), C. andersoni (2), and C. hominis (2), and the predominant subtype was C. parvum IIa A17G2R1. Assuming USA regulations for surface water-supplied PWS were applicable to the study wells, wells positive for Cryptosporidium by IFA would likely be required to add treatment. Cryptosporidium is not uncommon in groundwater, even when surface water in\ufb02uence is absent.", "description": "8 p.", "publisher": { "@type": "Organization", "name": "American Chemical Society" }, "author": [ { "@type": "Person", "name": "Borchardt, Mark A.", "givenName": "Mark A.", "familyName": "Borchardt", "identifier": { "@type": "PropertyValue", "propertyID": "ORCID", "value": "0000-0002-6471-2627", "url": "https://orcid.org/0000-0002-6471-2627" }, "affiliation": [ { "@type": "Organization", "name": "United States Department of Agriculture Agricultural Research Service" } ] }, { "@type": "Person", "name": "de Lambert, Jane R.", "givenName": "Jane R.", "familyName": "de Lambert" }, { "@type": "Person", "name": "Fimstahl, Aaron D.", "givenName": "Aaron D.", "familyName": "Fimstahl", "identifier": { "@type": "PropertyValue", "propertyID": "ORCID", "value": "0000-0003-2686-7596", "url": "https://orcid.org/0000-0003-2686-7596" }, "affiliation": [ { "@type": "Organization", "name": "Upper Midwest Water Science Center" } ] }, { "@type": "Person", "name": "Stokdyk, Joel P. jstokdyk@usgs.gov", "givenName": "Joel P.", "familyName": "Stokdyk", "email": "jstokdyk@usgs.gov", "identifier": { "@type": "PropertyValue", "propertyID": "ORCID", "value": "0000-0003-2887-6277", "url": "https://orcid.org/0000-0003-2887-6277" }, "affiliation": [ { "@type": "Organization", "name": "Wisconsin Water Science Center", "url": "https://www.usgs.gov/centers/upper-midwest-water-science-center" }, { "@type": "Organization", "name": "Upper Midwest Water Science Center", "url": "https://www.usgs.gov/centers/upper-midwest-water-science-center" } ] }, { "@type": "Person", "name": "Spencer, Susan K.", "givenName": "Susan K.", "familyName": "Spencer" }, { "@type": "Person", "name": "Walsh, James F. ", "givenName": "James F. ", "familyName": "Walsh" }, { "@type": "Person", "name": "Anderson, Anita C.", "givenName": "Anita C.", "familyName": "Anderson" }, { "@type": "Person", "name": "Rezania, Lih-in W.", "givenName": "Lih-in W.", "familyName": "Rezania" } ], "funder": [ { "@type": "Organization", "name": "Upper Midwest Water Science Center", "url": "https://www.usgs.gov/centers/upper-midwest-water-science-center" } ], "spatialCoverage": [ { "@type": "Place", "additionalType": "country", "name": "United States", "url": "https://geonames.org/4074035" }, { "@type": "Place", "additionalType": "state", "name": "Minnesota" }, { "@type": "Place", "geo": [ { "@type": "GeoShape", "additionalProperty": { "@type": "PropertyValue", "name": "GeoJSON", "value": { "type": "FeatureCollection", "features": [ { "type": "Feature", "geometry": { "type": "Polygon", "coordinates": [ [ [ -92.204691, 46.704041 ], [ -92.205192, 46.698341 ], [ -92.183091, 46.695241 ], [ -92.176091, 46.686341 ], [ -92.204092, 46.666941 ], [ 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"abstract_inverted_index": { "Regulations": [ 0 ], "for": [ 1, 62, 173, 185 ], "public": [ 2 ], "water": [ 3, 16, 26, 43, 71, 128, 205 ], "systems": [ 4 ], "(PWS)": [ 5 ], "in": [ 6, 37, 41, 200 ], "the": [ 7, 33, 79, 143, 162, 180 ], "U.S.": [ 8 ], "consider": [ 9 ], "Cryptosporidium": [ 10, 23, 36, 63, 107, 121, 186, 196 ], "a": [ 11 ], "microbial": [ 12 ], "contaminant": [ 13 ], "of": [ 14, 22, 35, 82, 99, 142 ], "surface": [ 15, 25, 42, 127, 174, 204 ], "supplies.": [ 17 ], "Groundwater": [ 18 ], "is": [ 19, 27, 197, 207 ], "assumed": [ 20 ], "free": [ 21 ], "unless": [ 24 ], "entering": [ 28 ], "supply": [ 29 ], "wells.": [ 30 ], "We": [ 31, 138 ], "determined": [ 32 ], "incidence": [ 34, 122 ], "PWS": [ 38, 48, 176 ], "wells": [ 39, 49, 88, 183 ], "varying": [ 40 ], "influence.": [ 44 ], "Community": [ 45 ], "and": [ 46, 60, 66, 78, 90, 98, 112, 157, 161 ], "noncommunity": [ 47 ], "(": [ 50, 56, 117 ], "n": [ 51, 57 ], "=": [ 52, 58 ], "145)": [ 53 ], "were": [ 54, 94, 103, 114, 177 ], "sampled": [ 55 ], "964)": [ 59 ], "analyzed": [ 61 ], "by": [ 64, 75, 96, 105, 110, 132, 187 ], "qPCR": [ 65, 111 ], "immunofluorescence": [ 67 ], "assay": [ 68 ], "(IFA).": [ 69 ], "Surface": [ 70 ], "influence": [ 72, 129, 206 ], "was": [ 73, 123, 165 ], "assessed": [ 74, 131 ], "stable": [ 76, 133 ], "isotopes": [ 77, 134 ], "expert": [ 80, 136 ], "judgment": [ 81 ], "hydrogeologists": [ 83 ], "using": [ 84 ], "site-specific": [ 85 ], "data.": [ 86 ], "Fifty-eight": [ 87 ], "(40%)": [ 89 ], "107": [ 91, 144 ], "samples": [ 92, 101, 146 ], "(11%)": [ 93 ], "Cryptosporidium-positive": [ 95 ], "qPCR,": [ 97 ], "these": [ 100 ], "67": [ 102 ], "positive": [ 104, 145, 184 ], "IFA.": [ 106 ], "concentrations": [ 108 ], "measured": [ 109 ], "IFA": [ 113, 188 ], "significantly": [ 115 ], "correlated": [ 116 ], "p": [ 118 ], "<": [ 119 ], "0.001).": [ 120 ], "not": [ 124, 198 ], "associated": [ 125 ], "with": [ 126 ], "as": [ 130 ], "or": [ 135 ], "judgment.": [ 137 ], "successfully": [ 139 ], "sequenced": [ 140 ], "45": [ 141 ], "to": [ 147, 179, 193 ], "identify": [ 148 ], "species,": [ 149 ], "including": [ 150 ], "C.": [ 151, 154, 158, 166 ], "parvum": [ 152, 167 ], "(41),": [ 153 ], "andersoni": [ 155 ], "(2),": [ 156, 160 ], "hominis": [ 159 ], "predominant": [ 163 ], "subtype": [ 164 ], "IIa": [ 168 ], "A17G2R1.": [ 169 ], "Assuming": [ 170 ], "USA": [ 171 ], "regulations": [ 172 ], "water-supplied": [ 175 ], "applicable": [ 178 ], "study": [ 181 ], "wells,": [ 182 ], "would": [ 189 ], "likely": [ 190 ], "be": [ 191 ], "required": [ 192 ], "add": [ 194 ], "treatment.": [ 195 ], "uncommon": [ 199 ], "groundwater,": [ 201 ], "even": [ 202 ], "when": [ 203 ], "absent.": [ 208 ] }, "apc_list": null, "apc_paid": null, "authorships": [ { "author_position": "first", "author": { "id": "https://openalex.org/A5038389962", "display_name": "Joel P. 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