Item talk:Q258249

{

 "USGS Publications Warehouse": {
   "@context": "https://schema.org",
   "@type": "Article",
   "additionalType": "Journal Article",
   "name": "Differences in antibody responses against Chelonid Alphaherpesvirus 5 (ChHV5) suggest differences in virus biology in ChHV5-seropositive green turtles from Hawaii and ChHV5-seropositive green turtles from Florida",
   "identifier": [
     {
       "@type": "PropertyValue",
       "propertyID": "USGS Publications Warehouse IndexID",
       "value": "70245270",
       "url": "https://pubs.usgs.gov/publication/70245270"
     },
     {
       "@type": "PropertyValue",
       "propertyID": "USGS Publications Warehouse Internal ID",
       "value": 70245270
     },
     {
       "@type": "PropertyValue",
       "propertyID": "DOI",
       "value": "10.1128/JVI.01658-19",
       "url": "https://doi.org/10.1128/JVI.01658-19"
     }
   ],
   "journal": {
     "@type": "Periodical",
     "name": "Journal of Virology",
     "volumeNumber": "94",
     "issueNumber": "4"
   },
   "inLanguage": "en",
   "isPartOf": [
     {
       "@type": "CreativeWorkSeries",
       "name": "Journal of Virology"
     }
   ],
   "datePublished": "2020",
   "dateModified": "2023-06-21",
   "abstract": "Fibropapillomatosis (FP) is a tumor disease associated with a herpesvirus (chelonid herpesvirus 5 [ChHV5]) that affects mainly green turtles globally. Understanding the epidemiology of FP has been hampered by a lack of robust serological assays to monitor exposure to ChHV5. This is due in part to an inability to efficiently culture the virus\u00a0in vitro\u00a0for neutralization assays. Here, we expressed two glycoproteins (FUS4 and FUS8) from ChHV5 using baculovirus. These proteins were immobilized on enzyme-linked immunosorbent assay plates in their native form and assayed for reactivity to two types of antibodies, full-length 7S IgY and 5.7S IgY, which has a truncated Fc region. Turtles from Florida were uniformly seropositive to ChHV5 regardless of tumor status. In contrast, in turtles from Hawaii, we detected strong antibody reactivity mainly in tumored animals, with a lower antibody response being seen in nontumored animals, including those from areas where FP is enzootic. Turtles from Hawaii actively shedding ChHV5 were more seropositive than nonshedders. In trying to account for differences in the serological responses to ChHV5 between green turtles from Hawaii and green turtles from Florida, we rejected the cross-reactivity of antibodies to other herpesviruses, differences in viral epitopes, or differences in procedure as likely explanations. Rather, behavioral or other differences between green turtles from Hawaii and green turtles from Florida might have led to the emergence of biologically different viral strains. While the strains from turtles in Florida apparently spread independently of tumors, the transmission of the Hawaiian subtype relies heavily on tumor formation.",
   "description": "e01658-19, 15 p.",
   "publisher": {
     "@type": "Organization",
     "name": "ASM Journals"
   },
   "author": [
     {
       "@type": "Person",
       "name": "Work, Thierry M. thierry_work@usgs.gov",
       "givenName": "Thierry M.",
       "familyName": "Work",
       "email": "thierry_work@usgs.gov",
       "identifier": {
         "@type": "PropertyValue",
         "propertyID": "ORCID",
         "value": "0000-0002-4426-9090",
         "url": "https://orcid.org/0000-0002-4426-9090"
       },
       "affiliation": [
         {
           "@type": "Organization",
           "name": "National Wildlife Health Center",
           "url": "https://www.usgs.gov/centers/nwhc"
         }
       ]
     },
     {
       "@type": "Person",
       "name": "Dagenais, Julie jdagenais@usgs.gov",
       "givenName": "Julie",
       "familyName": "Dagenais",
       "email": "jdagenais@usgs.gov",
       "identifier": {
         "@type": "PropertyValue",
         "propertyID": "ORCID",
         "value": "0000-0001-5560-9946",
         "url": "https://orcid.org/0000-0001-5560-9946"
       },
       "affiliation": [
         {
           "@type": "Organization",
           "name": "National Wildlife Health Center",
           "url": "https://www.usgs.gov/centers/nwhc"
         }
       ]
     },
     {
       "@type": "Person",
       "name": "Willimann, Anna",
       "givenName": "Anna",
       "familyName": "Willimann",
       "affiliation": [
         {
           "@type": "Organization",
           "name": "University of Zurich"
         }
       ]
     },
     {
       "@type": "Person",
       "name": "Balazs, George",
       "givenName": "George",
       "familyName": "Balazs",
       "affiliation": [
         {
           "@type": "Organization",
           "name": "Golden Honu Services"
         }
       ]
     },
     {
       "@type": "Person",
       "name": "Mansfield, Kate",
       "givenName": "Kate",
       "familyName": "Mansfield",
       "affiliation": [
         {
           "@type": "Organization",
           "name": "University of Central Florida"
         }
       ]
     },
     {
       "@type": "Person",
       "name": "Ackermann, Mathias",
       "givenName": "Mathias",
       "familyName": "Ackermann",
       "affiliation": [
         {
           "@type": "Organization",
           "name": "University of Zurich"
         }
       ]
     }
   ],
   "funder": [
     {
       "@type": "Organization",
       "name": "National Wildlife Health Center",
       "url": "https://www.usgs.gov/centers/nwhc"
     }
   ],
   "spatialCoverage": [
     {
       "@type": "Place",
       "additionalType": "country",
       "name": "United States",
       "url": "https://geonames.org/4074035"
     },
     {
       "@type": "Place",
       "additionalType": "state",
       "name": "Florida"
     },
     {
       "@type": "Place",
       "additionalType": "state",
       "name": "Hawaii"
     }
   ]
 }

}