Item talk:Q256490

{

 "USGS Publications Warehouse": {
   "@context": "https://schema.org",
   "@type": "Article",
   "additionalType": "Journal Article",
   "name": "Sperm quality assessments for endangered razorback suckers Xyrauchen texanus",
   "identifier": [
     {
       "@type": "PropertyValue",
       "propertyID": "USGS Publications Warehouse IndexID",
       "value": "70003614",
       "url": "https://pubs.usgs.gov/publication/70003614"
     },
     {
       "@type": "PropertyValue",
       "propertyID": "USGS Publications Warehouse Internal ID",
       "value": 70003614
     },
     {
       "@type": "PropertyValue",
       "propertyID": "DOI",
       "value": "10.1530/REP-10-0153",
       "url": "https://doi.org/10.1530/REP-10-0153"
     }
   ],
   "journal": {
     "@type": "Periodical",
     "name": "Reproduction",
     "volumeNumber": "141",
     "issueNumber": "1"
   },
   "inLanguage": "en",
   "isPartOf": [
     {
       "@type": "CreativeWorkSeries",
       "name": "Reproduction"
     }
   ],
   "datePublished": "2011",
   "dateModified": "2021-05-20",
   "abstract": "Flow cytometry (FCM) and computer-assisted sperm motion analysis (CASA) methods were developed and validated for use with endangered razorback suckers Xyrauchen texanus collected (n=64) during the 2006 spawning season. Sperm motility could be activated within osmolality ranges noted during milt collections (here 167\u2013343 mOsm/kg). We hypothesized that sperm quality of milt collected into isoosmotic (302 mOsm/kg) or hyperosmotic (500 mOsm/kg) Hanks' balanced salt solution would not differ. Pre-freeze viabilities were similar between osmolalities (79%\u00b16 (S.E.M.) and 76%\u00b17); however, post-thaw values were greater in hyperosmotic buffer (27%\u00b13 and 12%\u00b12; P=0.0065), as was mitochondrial membrane potential (33%\u00b14 and 13%\u00b12; P=0.0048). Visual estimates of pre-freeze motility correlated with total (r=0.7589; range 23\u201382%) and progressive motility (r=0.7449) by CASA and were associated with greater viability (r=0.5985; P<0.0001). Count (FCM) was negatively correlated with post-thaw viability (r=-0.83; P=0.0116) and mitochondrial function (r=-0.91; P=0.0016). By FCM-based assessments of DNA integrity, whereby increased fluorochrome binding indicated more fragmentation, higher levels were negatively correlated with count (r=-0.77; P<0.0001) and pre-freeze viabilities (r=-0.66; P=0.0004). Fragmentation was higher in isotonic buffer (P=0.0234). To increase reproductive capacity of natural populations, the strategy and protocols developed can serve as a template for use with other imperiled fish species, biomonitoring, and genome banking.",
   "description": "11 p.",
   "publisher": {
     "@type": "Organization",
     "name": "Society for Reproduction and Fertility"
   },
   "author": [
     {
       "@type": "Person",
       "name": "Jenkins, Jill A. jenkinsj@usgs.gov",
       "givenName": "Jill A.",
       "familyName": "Jenkins",
       "email": "jenkinsj@usgs.gov",
       "identifier": {
         "@type": "PropertyValue",
         "propertyID": "ORCID",
         "value": "0000-0002-5087-0894",
         "url": "https://orcid.org/0000-0002-5087-0894"
       },
       "affiliation": [
         {
           "@type": "Organization",
           "name": "Wetland and Aquatic Research Center",
           "url": "https://www.usgs.gov/centers/wetland-and-aquatic-research-center"
         },
         {
           "@type": "Organization",
           "name": "National Wetlands Research Center",
           "url": "https://www.usgs.gov/centers/wetland-and-aquatic-research-center"
         }
       ]
     },
     {
       "@type": "Person",
       "name": "Eilts, Bruce E.",
       "givenName": "Bruce E.",
       "familyName": "Eilts",
       "identifier": {
         "@type": "PropertyValue",
         "propertyID": "ORCID",
         "value": "0000-0003-1087-0328",
         "url": "https://orcid.org/0000-0003-1087-0328"
       }
     },
     {
       "@type": "Person",
       "name": "Draugelis-Dale, Rassa O. daler@usgs.gov",
       "givenName": "Rassa O.",
       "familyName": "Draugelis-Dale",
       "email": "daler@usgs.gov",
       "identifier": {
         "@type": "PropertyValue",
         "propertyID": "ORCID",
         "value": "0000-0001-8532-3287",
         "url": "https://orcid.org/0000-0001-8532-3287"
       },
       "affiliation": [
         {
           "@type": "Organization",
           "name": "Wetland and Aquatic Research Center",
           "url": "https://www.usgs.gov/centers/wetland-and-aquatic-research-center"
         },
         {
           "@type": "Organization",
           "name": "National Wetlands Research Center",
           "url": "https://www.usgs.gov/centers/wetland-and-aquatic-research-center"
         }
       ]
     },
     {
       "@type": "Person",
       "name": "Guitreau, Amy M.",
       "givenName": "Amy M.",
       "familyName": "Guitreau"
     },
     {
       "@type": "Person",
       "name": "Figiel, Chester R.",
       "givenName": "Chester R.",
       "familyName": "Figiel"
     },
     {
       "@type": "Person",
       "name": "Tiersch, Terrence R.",
       "givenName": "Terrence R.",
       "familyName": "Tiersch",
       "identifier": {
         "@type": "PropertyValue",
         "propertyID": "ORCID",
         "value": "0000-0002-7738-1978",
         "url": "https://orcid.org/0000-0002-7738-1978"
       }
     }
   ],
   "funder": [
     {
       "@type": "Organization",
       "name": "National Wetlands Research Center",
       "url": "https://www.usgs.gov/centers/wetland-and-aquatic-research-center"
     }
   ]
 }

}