Item talk:Q256322

{

 "USGS Publications Warehouse": {
   "@context": "https://schema.org",
   "@type": "Article",
   "additionalType": "Journal Article",
   "name": "Elicitation of macrophages from the peritoneal cavity of channel catfish",
   "identifier": [
     {
       "@type": "PropertyValue",
       "propertyID": "USGS Publications Warehouse IndexID",
       "value": "70020701",
       "url": "https://pubs.usgs.gov/publication/70020701"
     },
     {
       "@type": "PropertyValue",
       "propertyID": "USGS Publications Warehouse Internal ID",
       "value": 70020701
     },
     {
       "@type": "PropertyValue",
       "propertyID": "DOI",
       "value": "10.1577/1548-8667(1998)010<0069:EOMFTP>2.0.CO;2",
       "url": "https://doi.org/10.1577/1548-8667(1998)010<0069:EOMFTP>2.0.CO;2"
     },
     {
       "@type": "PropertyValue",
       "propertyID": "ISSN",
       "value": "08997659"
     }
   ],
   "journal": {
     "@type": "Periodical",
     "name": "Journal of Aquatic Animal Health",
     "volumeNumber": "10",
     "issueNumber": "1"
   },
   "inLanguage": "en",
   "isPartOf": [
     {
       "@type": "CreativeWorkSeries",
       "name": "Journal of Aquatic Animal Health"
     }
   ],
   "datePublished": "1998",
   "dateModified": "2020-09-01",
   "abstract": "Four chemicals were evaluated for elicitation of macrophages in peritoneal cavities of 250\u2013300\u2010g healthy channel catfish\u00a0Ictalurus punctatus.\u00a0Cellular exudates were collected at 3, 5, 7, 10, 14, and 20 d following intraperitoneal injections with squalene, Freund's incomplete adjuvant (FIA), goat serum, thioglycollate, or as a control, phosphate\u2010buffered saline. Injection with either squalene or FIA induced significantly greater (P\u00a0\u2a7d 0.0001) macrophage recruitment than the other chemicals. The effectiveness of squalene and FIA was compared further by macrophage collection daily for 7 d. Squalene and FIA elicited similarly high macrophage responses (P\u00a0\u2a7d 0.0450), the highest being 3.43 \u00d7 106\u00a0macrophages/mL (SE, 2.4 \u00d7 106) at 99% purity at day 2 and 2.1 \u00d7 106\u00a0macrophages/mL (SE, 0.7 \u00d7 106) at day 14 at 80% purity, respectively. In both experiments, the time after injection was not statistically significant, nor was there an interaction between time and chemicals. The occurrence of cells other than macrophages decreased with time to yield macrophage recoveries of 47\u201399% for squalene and 30\u201380% for FIA. Two subsets of macrophages were observed by means of flow cytometry. As demonstrated by chemiluminescence, the squalene\u2010elicited cells produced high\u2010energy oxygen compounds important to the phagocytic process.",
   "description": "6 p.",
   "publisher": {
     "@type": "Organization",
     "name": "American Fisheries Society"
   },
   "author": [
     {
       "@type": "Person",
       "name": "Jenkins, J.A.",
       "givenName": "J.A.",
       "familyName": "Jenkins",
       "identifier": {
         "@type": "PropertyValue",
         "propertyID": "ORCID",
         "value": "0000-0002-5087-0894",
         "url": "https://orcid.org/0000-0002-5087-0894"
       },
       "affiliation": [
         {
           "@type": "Organization",
           "name": "Wetland and Aquatic Research Center",
           "url": "https://www.usgs.gov/centers/wetland-and-aquatic-research-center"
         }
       ]
     },
     {
       "@type": "Person",
       "name": "Klesius, P.H.",
       "givenName": "P.H.",
       "familyName": "Klesius"
     }
   ],
   "funder": [
     {
       "@type": "Organization",
       "name": "National Wetlands Research Center",
       "url": "https://www.usgs.gov/centers/wetland-and-aquatic-research-center"
     }
   ]
 }

}