Item talk:Q253542
From geokb
{
"USGS Publications Warehouse": { "@context": "https://schema.org", "@type": "Article", "additionalType": "Journal Article", "name": "High-throughput sequencing reveals distinct regional genetic structure among remaining populations of an endangered salt marsh plant in California", "identifier": [ { "@type": "PropertyValue", "propertyID": "USGS Publications Warehouse IndexID", "value": "70209829", "url": "https://pubs.usgs.gov/publication/70209829" }, { "@type": "PropertyValue", "propertyID": "USGS Publications Warehouse Internal ID", "value": 70209829 }, { "@type": "PropertyValue", "propertyID": "DOI", "value": "10.1007/s10592-020-01269-3", "url": "https://doi.org/10.1007/s10592-020-01269-3" } ], "journal": { "@type": "Periodical", "name": "Conservation Genetics", "volumeNumber": "21", "issueNumber": null }, "inLanguage": "en", "isPartOf": [ { "@type": "CreativeWorkSeries", "name": "Conservation Genetics" } ], "datePublished": "2020", "dateModified": "2020-06-04", "abstract": "Conservation of rare species requires careful consideration to both preserve locally adapted traits and maintain genetic diversity, as species\u2019 ranges fluctuate in response to a changing climate and habitat loss. Salt marsh systems in California have been highly modified and many salt marsh obligate species have undergone range reductions and habitat loss with concomitant losses of genetic diversity and connectivity. Remaining salt marshes are threatened by rising sea levels, and so these habitats will likely require active restoration and re-establishment efforts. This study aims to provide a reference point for the current status of genetic diversity and range-wide population structure of a federally and state listed endangered plant, Salt Marsh Bird\u2019s Beak (Chloropyron maritimum subsp. maritimum) that can inform future preservation and restoration efforts. We used historical data and current monitoring information to locate and sample all known occurrences throughout the species range in Southern California, and three additional occurrences from Baja California, Mexico. We used flow cytometry and single nucleotide polymorphic markers (SNPs), generated by double-digest restriction-site associated DNA sequencing (ddRAD), to assess relative ploidy, and estimate genetic diversity and population structure across the region. Overall, we found four to five distinct genetic clusters that coincide with geographic regions. Genetic diversity was greatest in the southern part of the range including Baja California and San Diego. 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