Item talk:Q253295
From geokb
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"name": "Development of microsatellite markers for three at risk tiger beetles Cicindela dorsalis dorsalis, C. d. media, and C. puritana",
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"abstract": "ObjectiveTiger beetles inhabiting sandy beaches and cliffs along the east coast of the United States are facing increasing habitat loss due to erosion, urbanization, and sea level rise. The northeastern beach tiger beetle\u00a0Cicindela dorsalis dorsalis\u00a0and Puritan tiger beetle\u00a0Cicindela puritana\u00a0are both listed as threatened under the Endangered Species Act\u00a0of 1973, while the white beach tiger beetle\u00a0Cicindela dorsalis media\u00a0is not listed but has been declining. Extirpation of these beetles, in some cases from entire states, has isolated many populations reducing gene flow and elevating the risk for the loss of genetic variation. To facilitate investigations of population genetic structure, we developed suites of microsatellite loci for conservation genetic studies.ResultsShotgun genomic sequencing of all species identified thousands of candidate microsatellite loci, among which 17 loci were optimized and verified to cross-amplify within\u00a0C. d. media\u00a0and\u00a0C. d. dorsalis, and eight separate loci were optimized for\u00a0C. puritana. Most loci conformed to Hardy\u2013Weinberg equilibrium, showed no evidence of linkage disequilibrium or null alleles, and revealed population genetic characteristics informative for natural resource managers among the populations tested.",
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