Item talk:Q247996
From geokb
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"name": "Application of non-lethal stable isotope analysis to assess feeding patterns of juvenile pallid sturgeon Scaphirhynchus albus: a comparison of tissue types and sample preservation methods",
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"abstract": "Traditional techniques for stable isotope analysis (SIA) generally require sacrificing animals to collect tissue samples; this can be problematic when studying diets of endangered species such as the pallid sturgeon\u00a0Scaphirhynchus albus. Our objectives were to (i) determine if pectoral fin tissue (non-lethal) could be a substitute for muscle tissue (lethal) in SIA of juvenile pallid sturgeon, and (ii) evaluate the influence of preservation techniques on stable isotope values. In the laboratory, individual juvenile pallid sturgeon were held for up to 186\u00a0day and fed chironomids, fish, or a commercially available pellet diet. Significant, positive relationships (r\u00b2\u00a0\u2265\u00a00.8) were observed between fin and muscle tissues for both \u03b415N and \u03b413C; in all samples isotopes were enriched in fins compared to muscle tissue. Chironomid and fish based diets of juvenile pallid sturgeon were distinguishable for fast growing fish (0.3\u00a0mm\u00a0day\u22121) using stable \u03b415N and \u03b413C isotopes. Frozen and preserved fin tissue \u03b415N isotopes were strongly related (r2\u00a0=\u00a00.89) but \u03b413C isotopes were weakly related (r2\u00a0=\u00a00.16). Therefore, freezing is recommended for preservation of fin clips to avoid the confounding effect of enrichment by ethanol. This study demonstrates the utility of a non-lethal technique to assess time integrated food habits of juvenile pallid sturgeon and should be applicable to other threatened or endangered species.",
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