Item talk:Q246439
From geokb
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"name": "Genetic characterization of Zostera asiatica on the Pacific Coast of North America",
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"abstract": "We gathered sequence information from the nuclear 5.8S rDNA gene and associated internal transcribed spacers, ITS-1 and ITS-2 (5.8S rDNA/ITS), and the chloroplast maturase K (matK) gene, from\u00a0Zostera\u00a0samples collected from subtidal habitats in Monterey and Santa Barbara (Isla Vista) bays, California, to test the hypothesis that these plants are conspecific with\u00a0Z. asiatica\u00a0Miki of Asia. Sequences from approximately 520 base pairs of the nuclear 5.8S rDNA/ITS obtained from the subtidal Monterey and Isla Vista\u00a0Zostera\u00a0samples were identical to homologous sequences obtained from\u00a0Z. marina\u00a0collected from intertidal habitats in Japan, Alaska, Oregon and California. Similarly, sequences from the\u00a0matK\u00a0gene from the subtidal\u00a0Zostera\u00a0samples were identical to\u00a0matK\u00a0sequences obtained from\u00a0Z. marina\u00a0collected from intertidal habitats in Japan, Alaska, Oregon and California, but differed from\u00a0Z. asiatica\u00a0sequences accessioned into GenBank. This suggests the subtidal plants are conspecific with\u00a0Z. marina, not\u00a0Z. asiatica. However, we found that herbarium samples accessioned into the Kyoto University Herbarium, determined to be\u00a0Z. asiatica, yielded 5.8S rDNA/ITS sequences consistent with either\u00a0Z. japonica, in two cases, or\u00a0Z. marina, in one case. Similar results were observed for the chloroplast\u00a0matK\u00a0gene; we found haplotypes that were inconsistent with published\u00a0matK\u00a0sequences from\u00a0Z. asiatica\u00a0collected from Japan. These results underscore the need for closer examination of the relationship between\u00a0Z. marina\u00a0along the Pacific Coast of North America, and\u00a0Z. asiatica\u00a0of Asia, for the retention and verification of specimens examined in scientific studies, and for assessment of the usefulness of morphological characters in the determination of taxonomic relationships within Zosteraceae.",
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