Item talk:Q245442
From geokb
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Measurement of TGF-\u03b2 mRNA expression level was one of the indicators chosen to evaluate juvenile sucker health during the restoration process. TGF-\u03b2 mRNA expression level has been correlated with disease status in several laboratory studies and TGF-\u03b2 mRNA expression level has been used as a species-specific indicator of immune status in field-based fish health assessments. We describe here the identification of TGF-\u03b2 and a possible splice variant from shortnose sucker and from Lost River sucker. The performance of a quantitative RT-PCR assay to measure TGF-\u03b2 mRNA expression level was evaluated in field-collected spleen and kidney tissue samples. The quality of extracted RNA was higher in tissues harvested in September compared to July and higher in tissues harvested at lower temperature compared to higher temperature. In addition, the expression level of both TGF-\u03b2 and 18S as assessed by qRT-PCR was higher in samples with higher quality RNA. 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