Item talk:Q240660
From geokb
{
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This technique, adapted from methods developed for mammalian cells, operates on a similar biological principle to\u00a03H-thymidine incorporation, but circumvents the logistical and safety issues inherent with the radioactive label. Because it directly measures DNA proliferation, the assay has advantages over other colorimetric methods that may be strongly influenced by leucocyte metabolic status. Using BrdU incorporation followed by ELISA, we evaluate the responsiveness of rainbow trout (Oncorhynchus mykiss\u00a0[Walbaum]) leucocytes to the mammalian T-cell mitogen Concanavalin A (Con A) as well as the differential response of white perch (Morone americana\u00a0[Gmelin]) leucocytes to Con A and pokeweed mitogen. 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