Item talk:Q238526
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{
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"@type": "Article",
"additionalType": "Journal Article",
"name": "A new organic reference material, L-glutamic acid, USGS41a, for \u03b413C and \u03b415N measurements \u2212 a replacement for USGS41",
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"value": "10.1002/rcm.7510",
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"@type": "Periodical",
"name": "Rapid Communications in Mass Spectrometry",
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"abstract": "RationaleThe widely used l-glutamic acid isotopic reference material USGS41, enriched in both 13C and 15N, is nearly exhausted. A new material, USGS41a, has been prepared as a replacement for USGS41.MethodsUSGS41a was prepared by dissolving analytical grade l-glutamic acid enriched in 13C and 15N together with l-glutamic acid of normal isotopic composition. The \u03b413C and \u03b415N values of USGS41a were directly or indirectly normalized with the international reference materials NBS 19 calcium carbonate (\u03b413CVPDB = +1.95 mUr, where milliurey = 0.001 = 1 \u2030), LSVEC lithium carbonate (\u03b413CVPDB = \u221246.6 mUr), and IAEA-N-1 ammonium sulfate (\u03b415NAir = +0.43 mUr) and USGS32 potassium nitrate (\u03b415N = +180 mUr exactly) by on-line combustion, continuous-flow isotope-ratio mass spectrometry, and off-line dual-inlet isotope-ratio mass spectrometry.ResultsUSGS41a is isotopically homogeneous; the reproducibility of \u03b413C and \u03b415N is better than 0.07 mUr and 0.09 mUr, respectively, in 200-\u03bcg amounts. It has a \u03b413C value of +36.55 mUr relative to VPDB and a \u03b415N value of +47.55 mUr relative to N2 in air. USGS41 was found to be hydroscopic, probably due to the presence of pyroglutamic acid. Experimental results indicate that the chemical purity of USGS41a is substantially better than that of USGS41.ConclusionsThe new isotopic reference material USGS41a can be used with USGS40 (having a \u03b413CVPDB value of \u221226.39 mUr and a \u03b415NAir value of \u22124.52 mUr) for (i) analyzing local laboratory isotopic reference materials, and (ii) quantifying drift with time, mass-dependent isotopic fractionation, and isotope-ratio-scale contraction for isotopic analysis of biological and organic materials. Published in 2016. This article is a U.S. Government work and is in the public domain in the USA.",
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