Item talk:Q227273
From geokb
{
"@context": "http://schema.org/", "@type": "WebPage", "additionalType": "Project", "url": "https://www.usgs.gov/centers/norock/science/readi-net-providing-tools-early-detection-and-management-aquatic-invasive", "headline": "READI-Net: Providing Tools for the Early Detection and Management of Aquatic Invasive Species", "datePublished": "August 14, 2022", "author": [ { "@type": "Person", "name": "Adam Sepulveda, Ph.D.", "url": "https://www.usgs.gov/staff-profiles/adam-sepulveda", "identifier": { "@type": "PropertyValue", "propertyID": "orcid", "value": "0000-0001-7621-7028" } }, { "@type": "Person", "name": "Scott George", "url": "https://www.usgs.gov/staff-profiles/scott-george", "identifier": { "@type": "PropertyValue", "propertyID": "orcid", "value": "0000-0002-8197-1866" } }, { "@type": "Person", "name": "Chris M Merkes", "url": "https://www.usgs.gov/staff-profiles/chris-m-merkes", "identifier": { "@type": "PropertyValue", "propertyID": "orcid", "value": "0000-0001-8191-627X" } }, { "@type": "Person", "name": "Stephen Spear, PhD", "url": "https://www.usgs.gov/staff-profiles/stephen-spear", "identifier": { "@type": "PropertyValue", "propertyID": "orcid", "value": "0000-0001-8351-9382" } }, { "@type": "Person", "name": "Margaret Hunter, Ph.D.", "url": "https://www.usgs.gov/staff-profiles/margaret-hunter", "identifier": { "@type": "PropertyValue", "propertyID": "orcid", "value": "0000-0002-4760-9302" } }, { "@type": "Person", "name": "Matthew Neilson, Ph.D.", "url": "https://www.usgs.gov/staff-profiles/matthew-neilson", "identifier": { "@type": "PropertyValue", "propertyID": "orcid", "value": "0000-0002-5139-5677" } }, { "@type": "Person", "name": "Katy Klymus, PhD", "url": "https://www.usgs.gov/staff-profiles/katy-klymus", "identifier": { "@type": "PropertyValue", "propertyID": "orcid", "value": "0000-0002-8843-6241" } }, { "@type": "Person", "name": "David S Pilliod", "url": "https://www.usgs.gov/staff-profiles/david-s-pilliod", "identifier": { "@type": "PropertyValue", "propertyID": "orcid", "value": "0000-0003-4207-3518" } }, { "@type": "Person", "name": "Andy Royle, Ph.D.", "url": "https://www.usgs.gov/staff-profiles/andy-royle", "identifier": { "@type": "PropertyValue", "propertyID": "orcid", "value": "0000-0003-3135-2167" } }, { "@type": "Person", "name": "Cheryl L Morrison, Ph.D.", "url": "https://www.usgs.gov/staff-profiles/cheryl-l-morrison", "identifier": { "@type": "PropertyValue", "propertyID": "orcid", "value": "0000-0001-9425-691X" } }, { "@type": "Person", "name": "Aaron J Stephenson", "url": "https://www.usgs.gov/staff-profiles/aaron-j-stephenson", "identifier": { "@type": "PropertyValue", "propertyID": "orcid", "value": "0000-0002-0019-1607" } }, { "@type": "Person", "name": "Muruleedhara (Murulee) N Byappanahalli, PhD", "url": "https://www.usgs.gov/staff-profiles/muruleedhara-murulee-n-byappanahalli", "identifier": { "@type": "PropertyValue", "propertyID": "orcid", "value": "0000-0001-5376-597X" } } ], "description": [ { "@type": "TextObject", "text": "READI-Net is a set of tools designed with input from scientists and end-users to help new or current users of eDNA technology leverage tested protocols and best practices to collect, analyze, and share eDNA data that can assist managers, stakeholders, and decision-makers with their aquatic biological threat monitoring and research needs." }, { "@type": "TextObject", "text": "eDNA is DNA shed or excreted from living organisms into the environment, which makes it available to be sampled and monitored using new molecular methods. Using methods that rely on physically locating the few, often well-hidden individuals that comprise the first wave of an invasion into a new habitat can be difficult and costly. In contrast, sampling for the DNA of biological threats can be more effective and cost efficient." }, { "@type": "TextObject", "text": "Environmental DNA: An Emerging Tool for Understanding Aquatic Biodiversity" }, { "@type": "TextObject", "text": "Participating USGS Mission Areas:" }, { "@type": "TextObject", "text": "Second only to prevention, the National EDRR Framework considers early detection to be a cornerstone of biological threat management because it is critical in accomplishing three goals:" }, { "@type": "TextObject", "text": "Water Resources Mission Area" }, { "@type": "TextObject", "text": "406-404-9155" }, { "@type": "TextObject", "text": "asepulveda@usgs.gov" }, { "@type": "TextObject", "text": "FOR MORE INFORMATION:" }, { "@type": "TextObject", "text": "This eDNA-based effort, known as the USGS Rapid environmental DNA Assessment and Deployment Initiative & Network (READI-Net), puts eDNA technology into the hands of USGS partners and stakeholders to support early detection of and rapid response to aquatic biological threats such as invasive species, and human or wildlife pathogens and parasites." }, { "@type": "TextObject", "text": "Smith-Root" }, { "@type": "TextObject", "text": "The Department of Interior (DOI) and its partners have created the National Early Detection and Rapid Response (EDRR) Framework to reduce the impacts of biological threats. A focus of this strategy is developing and implementing an environmental DNA-based (eDNA) species detection and assessment framework." }, { "@type": "TextObject", "text": "Two robotic sampler options provide different sampling capabilities to meet a variety of user sampling strategies:" }, { "@type": "TextObject", "text": "Early detection is the process of surveying for, reporting, and verifying the presence of a biological threats, such as non-native species, before the founding population becomes established or spreads so widely that eradication is no longer feasible (DOI, 2016)." }, { "@type": "TextObject", "text": "Multi-species Analysis" }, { "@type": "TextObject", "text": "Laboratory Standards" }, { "@type": "TextObject", "text": "Northern Rocky Mountain Science Center" }, { "@type": "TextObject", "text": "READI-Net provides users with initial guidance to create sampling strategies that optimize detection of target species." }, { "@type": "TextObject", "text": "eDNA analysis is extremely sensitive to species presence and has become a rapid, cost-effective tool for species detection and monitoring, particularly in aquatic environments (Sepulveda et al., 2020). DNA from environmental samples (e.g., water, air, soil) can provide indirect evidence of a species\u2019 presence in a given environment, even when its numbers are low or the species is difficult to differentiate from close relatives (Marshall and Stepien, 2019). In comparison with traditional collection and identification methods in aquatic environments, such as netting, electrofishing, and visual surveys, eDNA techniques can be more sensitive, cost-effective, and targeted to the identification of species of interest; safer for wildlife and field staff; and less harmful to the ecosystem (Darling, 2019). By implementing eDNA techniques, resource managers can now reliably detect new aquatic biological threats before they have a chance to become well established, costly to manage, and ecologically or economically disruptive." }, { "@type": "TextObject", "text": "iTRACKDNA (University of Victoria and Institut National de La Recherche Scientifique)" }, { "@type": "TextObject", "text": "Biological threats are a serious concern for the United States as they can threaten native species, disrupt recreation, damage critical infrastructure, and impose millions of dollars of costs toward management and mitigation. The annual global cost imposed by biological threats is greater than $423 billion dollars and has quadrupled every decade since 1970 (IPBES, 2023). Biological threats are easily introduced and can have severe repercussions (case study)." }, { "@type": "TextObject", "text": "Learn more: Toward a national eDNA strategy for the United States" }, { "@type": "TextObject", "text": "Any number of species have the potential to invade a single site, therefore READI-Net is collaborating with partners to develop eDNA tools (e.g., high-throughput qPCR) that rapidly and simultaneously test a single sample for the DNA of many biological threats, rather than just a single species." }, { "@type": "TextObject", "text": "If you\u2019re currently using eDNA, or contemplating using eDNA for species detection, READI-Net is already designed for you. READI-Net complements traditional techniques to describe species distributions with the sensitivity, speed, and accuracy inherent in eDNA methodology. Using eDNA is especially effective in providing timely warning of potential invasion events to reduce downstream economic and environmental impacts. By compiling best practices across the many stages of eDNA surveillance, READI-Net removes guesswork for designing and implementing eDNA techniques to support early detection of invasive species." }, { "@type": "TextObject", "text": "What is eDNA? (video)" }, { "@type": "TextObject", "text": "Among the READI-Net laboratories, a single eDNA database tracking system is employed that utilizes a variety of software to track samples, automate workflows, and check for errors throughout the process. This sample tracking system provides individual sample tracking from initial collection, through analysis and reporting, and finally, to long-term storage." }, { "@type": "TextObject", "text": "Sample Tracking and Data Delivery" }, { "@type": "TextObject", "text": "Autonomous (robotic) eDNA Samplers" }, { "@type": "TextObject", "text": "Participating USGS Science Centers:" }, { "@type": "TextObject", "text": "Sampling Guidance" }, { "@type": "TextObject", "text": "Creation of READI-Net has been informed by stakeholders and subject matter experts to create a program that has wide user applicability. Outreach with a variety of users continues to expand the READI-Net project." }, { "@type": "TextObject", "text": "Related Links:" }, { "@type": "TextObject", "text": "READI-Net protocols recognize that it is imperative to process samples efficiently and provide data that are grounded in repeatable and reproducible lab workflows that meet critical quality management criteria." }, { "@type": "TextObject", "text": "READI-Net Collaborators (non-government)" }, { "@type": "TextObject", "text": "Learn more:" }, { "@type": "TextObject", "text": "Similar to the USGS Streamgaging Network, READI-Net provides a national framework to standardize eDNA methods and data management, creating a clearinghouse of data to protect resources and economies through invasive species management." }, { "@type": "TextObject", "text": "Building READI-Net Partnerships" }, { "@type": "TextObject", "text": "Monterey Bay Aquarium Research Institute (MBARI)" }, { "@type": "TextObject", "text": "Ecosystems Mission Area" }, { "@type": "TextObject", "text": "The USGS has developed the Rapid environmental (e)DNA Assessment and Deployment Initiative & Network (READI-Net) to accelerate the implementation of environmental DNA (eDNA) analysis as a best practice for the early detection of aquatic biological threats. READI-Net provides tools and a strategy to collect and deliver early detection data for natural resource managers and public health protection. Designed with stakeholder input, READI-Net maximizes the power and cost effectiveness of eDNA sampling for detecting biological threats by using autonomous robotic sample collection, multi-species analysis tools, strategic sample design, standardized and accountable lab analysis, and a communication framework for eDNA detection results. For users interested in implementing or continuing eDNA surveillance of aquatic biological threats, READI-Net provides support from start to finish, creating a network of standardized data which informs science, management, and policy." }, { "@type": "TextObject", "text": "Learn More:" }, { "@type": "TextObject", "text": "Adam Sepulveda, PhD, USGS Biologist, Lead READI-Net Investigator" }, { "@type": "TextObject", "text": "Columbia Environmental Research Center, Eastern Ecological Science Center, Forest and Rangeland Ecosystem Science Center, Fort Collins Science Center, Great Lakes Science Center, New York Water Science Center, Northern Rocky Mountain Science Center, Upper Midwest Environmental Sciences Center, Wetland and Aquatic Research Center, Wyoming-Montana Water Science Center" }, { "@type": "TextObject", "text": "Portable robotic eDNA samplers can be placed in the right place at the right time, programmed to sample any time of day or night, sample frequently (hourly or daily, for example), and can sample during hazardous conditions. READI-Net is collaborating with Monterey Bay Research Institute (MBARI) and Smith-Root to design and test robotic eDNA samplers that collect high quality samples and are affordable, scalable, and mobile. Designed with easy-to-use computer interfaces and wireless connectivity, researchers can control sampling remotely and safely." }, { "@type": "TextObject", "text": "READI-Net components (see polygons in READI-Net graphic) can be accessed individually or as the full suite, providing practical and financial flexibility and meeting stakeholders and partners where they are." }, { "@type": "TextObject", "text": "Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government." } ], "funder": { "@type": "Organization", "name": "Northern Rocky Mountain Science Center", "url": "https://www.usgs.gov/centers/norock" }, "about": [ { "@type": "Thing", "name": "Science Technology" }, { "@type": "Thing", "name": "Biochemistry and Physiology" }, { "@type": "Thing", "name": "Biology" }, { "@type": "Thing", "name": "Diseases of Aquatic Organisms" }, { "@type": "Thing", "name": "Water" }, { "@type": "Thing", "name": "eDNA" }, { "@type": "Thing", "name": "Bioassessment/Biomonitoring" }, { "@type": "Thing", "name": "Early Detection and Rapid Response" }, { "@type": "Thing", "name": "Control and Management" }, { "@type": "Thing", "name": "Fish and Wildlife Disease Investigation and Surveillance" }, { "@type": "Thing", "name": "Native Fish" }, { "@type": "Thing", "name": "Tools and Techniques" }, { "@type": "Thing", "name": "Invasive Species Monitoring and Detection" }, { "@type": "Thing", "name": "Fish and Wildlife Disease" }, { "@type": "Thing", "name": "Geology" }, { "@type": "Thing", "name": "Aquatic invasive species" }, { "@type": "Thing", "name": "early detection-rapid response" }, { "@type": "Thing", "name": "Energy" }, { "@type": "Thing", "name": "Invasive Animals" }, { "@type": "Thing", "name": "Invasive Species Detection and Control" }, { "@type": "Thing", "name": "Browse All Research Projects" }, { "@type": "Thing", "name": "Population Estimation" }, { "@type": "Thing", "name": "Statistical Analysis" }, { "@type": "Thing", "name": "Fish and Aquatic Species Conservation" }, { "@type": "Thing", "name": "Decision Science and Quantitative Methods" }, { "@type": "Thing", "name": "Biosurveillance" }, { "@type": "Thing", "name": "Ecosystems" }, { "@type": "Thing", "name": "Invasive Species" }, { "@type": "Thing", "name": "Environmental DNA" }, { "@type": "Thing", "name": "Invasive Pathogens" }, { "@type": "Thing", "name": "Aquatic Invasive Species Monitoring" }, { "@type": "Thing", "name": "Aquatic Ecosystems & Water" }, { "@type": "Thing", "name": "Biological threats" }, { "@type": "Thing", "name": "Aquatic Invasive Species" }, { "@type": "Thing", "name": "Information Systems" }, { "@type": "Thing", "name": "READI-Net" }, { "@type": "Thing", "name": "Methods and Analysis" }, { "@type": "Thing", "name": "Aquatic" }, { "@type": "Thing", "name": "Aquatic Biology" }, { "@type": "Thing", "name": "Environmental Health" }, { "@type": "Thing", "name": "Biosecurity" } ]
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