Item talk:Q226957

{

 "@context": "http://schema.org/",
 "@type": "WebPage",
 "additionalType": "Project",
 "url": "https://www.usgs.gov/centers/upper-midwest-environmental-sciences-center/science/sampling-design-landscape-level",
 "headline": "Sampling design for landscape level environmental DNA monitoring programs",
 "datePublished": "December 11, 2023",
 "author": [
   {
     "@type": "Person",
     "name": "Richard Erickson, PhD",
     "url": "https://www.usgs.gov/staff-profiles/richard-erickson",
     "identifier": {
       "@type": "PropertyValue",
       "propertyID": "orcid",
       "value": "0000-0003-4649-482X"
     }
   }
 ],
 "description": [
   {
     "@type": "TextObject",
     "text": "Researcher 1) determine how many samples are required to detect different species using eDNA, 2) examine when and where samples should be collected as well as how many samples should be taken to maximize the probability of collecting eDNA in the field and later detecting eDNA through PCR analysis, and 3) examine how well a 3-level occupancy model recovered known sample collection and detection parameters given different sample sizes."
   },
   {
     "@type": "TextObject",
     "text": "2630 Fanta Reed Road\nLa Crosse, WI 54603\nUnited States"
   },
   {
     "@type": "TextObject",
     "text": "This research is ongoing and collaborates with U.S. Fish and Wildlife Service and USGS scientist continue to assist with science to support eDNA-based monitoring."
   },
   {
     "@type": "TextObject",
     "text": "During traditional monitoring programs researchers often use occupancy models to quantity the probability a species occupies an area while accounting for imperfect detection. This approach uses as two-level model. Traditionally, presence or absence data are collected for species, however, recently scientists have started to use environmental DNA (eDNA) in monitoring programs. Examples of eDNA include skin cells, mucus, eggs, urine, or feces that organisms deposit into their surroundings. Sampling for eDNA can be a more efficient and less expensive way of detecting rare or elusive species compared to current standard methods (such as visual species counts, acoustic surveys). After a sample is collected, scientists use molecular techniques such as quantitative polymerase chain reaction (qPCR), which amplifies a targeted species\u2019 s DNA in the sample and allows researchers to determine if eDNA is present or absent. Due to this sampling design, a third level added to the traditional 2-level occupancy model to account for sampling. USGS scientists uses these models to help agencies such as U.S. Fish and Wildlife Service plan monitoring programs for species such as invasive carp."
   }
 ],
 "funder": {
   "@type": "Organization",
   "name": "Upper Midwest Environmental Sciences Center",
   "url": "https://www.usgs.gov/centers/upper-midwest-environmental-sciences-center"
 },
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