Item talk:Q308999

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{

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   "name": "Analysis of mitochondrial DNA sequence data demonstrates that monophyly of myotis occultus is complicated by greater sampling of myotis lucifugus",
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     "name": "Southwestern Naturalist",
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     "issueNumber": "4"
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   "abstract": "The validity of\u00a0Myotis occultus\u00a0as a species unique from\u00a0Myotis lucifugus\u00a0has been a source of debate. Most recently, many authorities treat\u00a0M. occultus\u00a0as a distinct species, at least in part because a previous study showed that\u00a0M. occultus\u00a0and\u00a0M. l. carissima\u00a0(the subspecies that occurs in closest geographic proximity to\u00a0M. occultus) form separate monophyletic clades based on sequences of two mitochondrial genes (cytochrome-b\u00a0[cytb] and cytochrome oxidase subunit II [COII]). We re-evaluated the phylogenetic relationship between\u00a0M. occultus\u00a0and\u00a0M. lucifugus\u00a0based on mitochondrial sequences using an expanded dataset of cytb and COII sequences that originated from more genetically diverse specimens of\u00a0M. lucifugus\u00a0collected across a broader geographic area. Based on a phylogenetic analysis, we found that\u00a0M. occultus\u00a0sublineages embedded within a well-supported clade that included some specimens of\u00a0M. lucifugus. These results indicate that the previous genetic analysis demonstrating that\u00a0M. occultus\u00a0and\u00a0M. lucifugus\u00a0form distinct monophyletic groups is unsupported by our larger dataset. Future research will likely need to focus on genetic work involving whole-genome sequencing of nuclear DNA to better resolve the true taxonomic relationship between\u00a0M. occultus\u00a0and\u00a0M. lucifugus.La valides de Myotis occultus como una especie distinta a Myotis lucifugus ha sido fuente de debate. Recientemente, muchas autoridades han considerado M. occultus como una especie diferente, en parte porque un estudio anterior mostr\u00f3 que M. occultus y M. l. carissima (la subespecie con la mayor proximidad geogr\u00e1fica a M. occultus) forman clados monofil\u00e9ticos separados basados en secuencias de dos genes mitocondriales (el citocromo-b [cytb] y la subunidad II de citocromo oxidasa [COII]). Nosotros hemos reevaluado la relaci\u00f3n filogen\u00e9tica entre M. occultus y M. lucifugus usando una ampliada colecci\u00f3n de datos que contiene secuencias de los genes mitocondriales cytb y COII de espec\u00edmenes de M. lucifugus gen\u00e9ticamente m\u00e1s diversos que fueron muestreados en un \u00e1rea geogr\u00e1fica m\u00e1s extensa. Nuestro an\u00e1lisis filogen\u00e9tico muestra que los sublinajes de M. occultus est\u00e1n incrustados dentro de un clado bien respaldado que incluye algunos espec\u00edmenes de M. lucifugus. Estos resultados indican que el an\u00e1lisis gen\u00e9tico anterior que demostr\u00f3 que M. occultus y M. lucifugus forman grupos monofil\u00e9ticos distintos no est\u00e1 respaldado por nuestra m\u00e1s amplia colecci\u00f3n de datos. Es probable que para resolver mejor la verdadera relaci\u00f3n taxon\u00f3mica entre M. occultus y M. lucifugus sea necesario el uso de secuenciaci\u00f3n del genoma completo del ADN nuclear.",
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