Item talk:Q256666
From geokb
{
"USGS Publications Warehouse": {
"@context": "https://schema.org",
"@type": "Article",
"additionalType": "Journal Article",
"name": "A novel technique for isolating DNA from Tempus\u2122 blood RNA tubes after RNA isolation",
"identifier": [
{
"@type": "PropertyValue",
"propertyID": "USGS Publications Warehouse IndexID",
"value": "70198561",
"url": "https://pubs.usgs.gov/publication/70198561"
},
{
"@type": "PropertyValue",
"propertyID": "USGS Publications Warehouse Internal ID",
"value": 70198561
},
{
"@type": "PropertyValue",
"propertyID": "DOI",
"value": "10.1186/s13104-018-3671-4",
"url": "https://doi.org/10.1186/s13104-018-3671-4"
}
],
"journal": {
"@type": "Periodical",
"name": "BMC Research Notes",
"volumeNumber": "11",
"issueNumber": null
},
"inLanguage": "en",
"isPartOf": [
{
"@type": "CreativeWorkSeries",
"name": "BMC Research Notes"
}
],
"datePublished": "2018",
"dateModified": "2018-08-07",
"abstract": "ObjectiveWe use Tempus blood RNA tubes (Applied Biosystems) during health assessments of American moose (Alces alces\u00a0spp.) as a minimally invasive means to obtain RNA. Here we describe a novel protocol to additionally isolate high-quality DNA from the supernatant remaining after the RNA isolation methodology. Metrics used to qualify DNA quality included measuring the concentration, obtaining a DNA integrity number from a genomic DNA ScreenTape assay (Agilent), and running the isolated DNA on an agarose gel.ResultsOf the 23 samples analyzed, the average DNA concentration was 121\u00a0ng/\u00b5l (range 4\u2013337\u00a0ng/\u00b5l) and a genomic DNA ScreenTape assay of seven samples indicated high DNA integrity values for 6 of the 7 samples (range 9.1\u20139.4 out of 10). Of the DNA sent for genotyping by sequencing, all proved to be of sufficient integrity to yield high-quality next-generation sequence results. We recommend this simple procedure to maximize the yield of both RNA and DNA from blood samples.",
"description": "Article 563; 5 p.",
"publisher": {
"@type": "Organization",
"name": "BMC"
},
"author": [
{
"@type": "Person",
"name": "Ferrante, Jason A. jferrante@usgs.gov",
"givenName": "Jason A.",
"familyName": "Ferrante",
"email": "jferrante@usgs.gov",
"identifier": {
"@type": "PropertyValue",
"propertyID": "ORCID",
"value": "0000-0003-3453-4636",
"url": "https://orcid.org/0000-0003-3453-4636"
},
"affiliation": [
{
"@type": "Organization",
"name": "Wetland and Aquatic Research Center",
"url": "https://www.usgs.gov/centers/wetland-and-aquatic-research-center"
}
]
},
{
"@type": "Person",
"name": "Giles, Michelle R.",
"givenName": "Michelle R.",
"familyName": "Giles",
"identifier": {
"@type": "PropertyValue",
"propertyID": "ORCID",
"value": "0000-0002-9806-3530",
"url": "https://orcid.org/0000-0002-9806-3530"
},
"affiliation": [
{
"@type": "Organization",
"name": "Wetland and Aquatic Research Center",
"url": "https://www.usgs.gov/centers/wetland-and-aquatic-research-center"
}
]
},
{
"@type": "Person",
"name": "Benzie, Emily",
"givenName": "Emily",
"familyName": "Benzie"
},
{
"@type": "Person",
"name": "Hunter, Margaret mhunter@usgs.gov",
"givenName": "Margaret",
"familyName": "Hunter",
"email": "mhunter@usgs.gov",
"identifier": {
"@type": "PropertyValue",
"propertyID": "ORCID",
"value": "0000-0002-4760-9302",
"url": "https://orcid.org/0000-0002-4760-9302"
},
"affiliation": [
{
"@type": "Organization",
"name": "Wetland and Aquatic Research Center",
"url": "https://www.usgs.gov/centers/wetland-and-aquatic-research-center"
},
{
"@type": "Organization",
"name": "Southeast Ecological Science Center",
"url": "https://www.usgs.gov/regions/southeast"
}
]
}
],
"funder": [
{
"@type": "Organization",
"name": "Wetland and Aquatic Research Center",
"url": "https://www.usgs.gov/centers/wetland-and-aquatic-research-center"
}
]
},
"OpenAlex": {
"_id": "https://openalex.org/w2886495745",
"abstract_inverted_index": {
"We": [
0,
137
],
"use": [
1
],
"Tempus": [
2
],
"blood": [
3,
152
],
"RNA": [
4,
42,
148
],
"tubes": [
5
],
"(Applied": [
6
],
"Biosystems)": [
7
],
"during": [
8
],
"health": [
9
],
"assessments": [
10
],
"of": [
11,
97,
107,
114,
128,
146
],
"American": [
12
],
"moose": [
13
],
"(Alces": [
14
],
"alces": [
15
],
"spp.)": [
16
],
"as": [
17
],
"a": [
18,
28,
56,
61,
92
],
"minimally": [
19
],
"invasive": [
20
],
"means": [
21
],
"to": [
22,
31,
47,
126,
131,
142
],
"obtain": [
23
],
"RNA.": [
24
],
"Here": [
25
],
"we": [
26
],
"describe": [
27
],
"novel": [
29
],
"protocol": [
30
],
"additionally": [
32
],
"isolate": [
33
],
"high-quality": [
34,
133
],
"DNA": [
35,
49,
57,
63,
71,
83,
94,
102,
118,
150
],
"from": [
36,
60,
151
],
"the": [
37,
41,
53,
69,
77,
81,
108,
117,
144
],
"supernatant": [
38
],
"remaining": [
39
],
"after": [
40
],
"isolation": [
43
],
"methodology.": [
44
],
"Metrics": [
45
],
"used": [
46
],
"qualify": [
48
],
"quality": [
50
],
"included": [
51
],
"measuring": [
52
],
"concentration,": [
54
],
"obtaining": [
55
],
"integrity": [
58,
103,
130
],
"number": [
59
],
"genomic": [
62,
93
],
"ScreenTape": [
64,
95
],
"assay": [
65,
96
],
"(Agilent),": [
66
],
"and": [
67,
91,
149
],
"running": [
68
],
"isolated": [
70
],
"on": [
72
],
"an": [
73
],
"agarose": [
74
],
"gel.": [
75
],
"Of": [
76,
116
],
"23": [
78
],
"samples": [
79,
99,
110
],
"analyzed,": [
80
],
"average": [
82
],
"concentration": [
84
],
"was": [
85
],
"121": [
86
],
"ng/\u00b5l": [
87
],
"(range": [
88,
111
],
"4\u2013337": [
89
],
"ng/\u00b5l)": [
90
],
"seven": [
98
],
"indicated": [
100
],
"high": [
101
],
"values": [
104
],
"for": [
105,
120
],
"6": [
106
],
"7": [
109
],
"9.1\u20139.4": [
112
],
"out": [
113
],
"10).": [
115
],
"sent": [
119
],
"genotyping": [
121
],
"by": [
122
],
"sequencing,": [
123
],
"all": [
124
],
"proved": [
125
],
"be": [
127
],
"sufficient": [
129
],
"yield": [
132,
145
],
"next-generation": [
134
],
"sequence": [
135
],
"results.": [
136
],
"recommend": [
138
],
"this": [
139
],
"simple": [
140
],
"procedure": [
141
],
"maximize": [
143
],
"both": [
147
],
"samples.": [
153
]
},
"apc_list": {
"value": 1110,
"currency": "GBP",
"value_usd": 1361,
"provenance": "doaj"
},
"apc_paid": {
"value": 1110,
"currency": "GBP",
"value_usd": 1361,
"provenance": "doaj"
},
"authorships": [
{
"author_position": "first",
"author": {
"id": "https://openalex.org/A5032375058",
"display_name": "Jason A. Ferrante",
"orcid": "https://orcid.org/0000-0003-3453-4636"
},
"institutions": [
{
"id": "https://openalex.org/I2801637956",
"display_name": "Cherokee Nation",
"ror": "https://ror.org/00p23dy23",
"country_code": "US",
"type": "government",
"lineage": [
"https://openalex.org/I2801637956"
]
},
{
"id": "https://openalex.org/I1286329397",
"display_name": "United States Geological Survey",
"ror": "https://ror.org/035a68863",
"country_code": "US",
"type": "government",
"lineage": [
"https://openalex.org/I1286329397",
"https://openalex.org/I1335927249"
]
}
],
"countries": [
"US"
],
"is_corresponding": false,
"raw_author_name": "Jason A. Ferrante",
"raw_affiliation_strings": [
"Cherokee Nation Technologies contracted to the U.S. Geological Survey, Wetland and Aquatic Research Center, 7920 NW 71st Street, Gainesville, FL, 32653, USA"
],
"affiliations": [
{
"raw_affiliation_string": "Cherokee Nation Technologies contracted to the U.S. Geological Survey, Wetland and Aquatic Research Center, 7920 NW 71st Street, Gainesville, FL, 32653, USA",
"institution_ids": [
"https://openalex.org/I2801637956",
"https://openalex.org/I1286329397"
]
}
]
},
{
"author_position": "middle",
"author": {
"id": "https://openalex.org/A5028795066",
"display_name": "Michelle R. Giles",
"orcid": null
},
"institutions": [
{
"id": "https://openalex.org/I1286329397",
"display_name": "United States Geological Survey",
"ror": "https://ror.org/035a68863",
"country_code": "US",
"type": "government",
"lineage": [
"https://openalex.org/I1286329397",
"https://openalex.org/I1335927249"
]
}
],
"countries": [
"US"
],
"is_corresponding": false,
"raw_author_name": "Michelle R. Giles",
"raw_affiliation_strings": [
"U.S. Geological Survey, Wetland and Aquatic Research Center, 7920 NW 71st Street, Gainesville, FL, 32653, USA"
],
"affiliations": [
{
"raw_affiliation_string": "U.S. Geological Survey, Wetland and Aquatic Research Center, 7920 NW 71st Street, Gainesville, FL, 32653, USA",
"institution_ids": [
"https://openalex.org/I1286329397"
]
}
]
},
{
"author_position": "middle",
"author": {
"id": "https://openalex.org/A5058775034",
"display_name": "Emily Benzie",
"orcid": null
},
"institutions": [
{
"id": "https://openalex.org/I84218800",
"display_name": "University of California, Davis",
"ror": "https://ror.org/05rrcem69",
"country_code": "US",
"type": "education",
"lineage": [
"https://openalex.org/I84218800"
]
}
],
"countries": [
"US"
],
"is_corresponding": false,
"raw_author_name": "Emily Benzie",
"raw_affiliation_strings": [
"University of California-Davis, 1 Shields Ave, Davis, CA, 95616, USA"
],
"affiliations": [
{
"raw_affiliation_string": "University of California-Davis, 1 Shields Ave, Davis, CA, 95616, USA",
"institution_ids": [
"https://openalex.org/I84218800"
]
}
]
},
{
"author_position": "last",
"author": {
"id": "https://openalex.org/A5034872140",
"display_name": "Margaret E. Hunter",
"orcid": "https://orcid.org/0000-0002-4760-9302"
},
"institutions": [
{
"id": "https://openalex.org/I1286329397",
"display_name": "United States Geological Survey",
"ror": "https://ror.org/035a68863",
"country_code": "US",
"type": "government",
"lineage": [
"https://openalex.org/I1286329397",
"https://openalex.org/I1335927249"
]
}
],
"countries": [
"US"
],
"is_corresponding": false,
"raw_author_name": "Margaret E. Hunter",
"raw_affiliation_strings": [
"U.S. Geological Survey, Wetland and Aquatic Research Center, 7920 NW 71st Street, Gainesville, FL, 32653, USA"
],
"affiliations": [
{
"raw_affiliation_string": "U.S. Geological Survey, Wetland and Aquatic Research Center, 7920 NW 71st Street, Gainesville, FL, 32653, USA",
"institution_ids": [
"https://openalex.org/I1286329397"
]
}
]
}
],
"best_oa_location": {
"is_oa": true,
"landing_page_url": "https://doi.org/10.1186/s13104-018-3671-4",
"pdf_url": "https://bmcresnotes.biomedcentral.com/track/pdf/10.1186/s13104-018-3671-4",
"source": {
"id": "https://openalex.org/S100014455",
"display_name": "BMC Research Notes",
"issn_l": "1756-0500",
"issn": [
"1756-0500"
],
"is_oa": true,
"is_in_doaj": true,
"is_core": true,
"host_organization": "https://openalex.org/P4310320256",
"host_organization_name": "BioMed Central",
"host_organization_lineage": [
"https://openalex.org/P4310319965",
"https://openalex.org/P4310320256"
],
"host_organization_lineage_names": [
"Springer Nature",
"BioMed Central"
],
"type": "journal"
},
"license": "cc-by",
"license_id": "https://openalex.org/licenses/cc-by",
"version": "publishedVersion",
"is_accepted": true,
"is_published": true
},
"biblio": {
"volume": "11",
"issue": "1",
"first_page": null,
"last_page": null
},
"citation_normalized_percentile": {
"value": 0.523479,
"is_in_top_1_percent": false,
"is_in_top_10_percent": false
},
"cited_by_api_url": "https://api.openalex.org/works?filter=cites:W2886495745",
"cited_by_count": 4,
"cited_by_percentile_year": {
"min": 78,
"max": 80
},
"concepts": [
{
"id": "https://openalex.org/C552990157",
"wikidata": "https://www.wikidata.org/wiki/Q7430",
"display_name": "DNA",
"level": 2,
"score": 0.71900654
},
{
"id": "https://openalex.org/C67705224",
"wikidata": "https://www.wikidata.org/wiki/Q11053",
"display_name": "RNA",
"level": 3,
"score": 0.68093663
},
{
"id": "https://openalex.org/C17757408",
"wikidata": "https://www.wikidata.org/wiki/Q4135516",
"display_name": "genomic DNA",
"level": 3,
"score": 0.58024263
},
{
"id": "https://openalex.org/C31467283",
"wikidata": "https://www.wikidata.org/wiki/Q912147",
"display_name": "Genotyping",
"level": 4,
"score": 0.55984145
},
{
"id": "https://openalex.org/C153911025",
"wikidata": "https://www.wikidata.org/wiki/Q7202",
"display_name": "Molecular biology",
"level": 1,
"score": 0.489718
},
{
"id": "https://openalex.org/C86803240",
"wikidata": "https://www.wikidata.org/wiki/Q420",
"display_name": "Biology",
"level": 0,
"score": 0.44172662
},
{
"id": "https://openalex.org/C2778476535",
"wikidata": "https://www.wikidata.org/wiki/Q390697",
"display_name": "Agarose",
"level": 2,
"score": 0.4109301
},
{
"id": "https://openalex.org/C112190416",
"wikidata": "https://www.wikidata.org/wiki/Q2124450",
"display_name": "RNA extraction",
"level": 4,
"score": 0.41076738
},
{
"id": "https://openalex.org/C51679486",
"wikidata": "https://www.wikidata.org/wiki/Q380546",
"display_name": "DNA sequencing",
"level": 3,
"score": 0.410486
},
{
"id": "https://openalex.org/C70721500",
"wikidata": "https://www.wikidata.org/wiki/Q177005",
"display_name": "Computational biology",
"level": 1,
"score": 0.32793435
},
{
"id": "https://openalex.org/C54355233",
"wikidata": "https://www.wikidata.org/wiki/Q7162",
"display_name": "Genetics",
"level": 1,
"score": 0.20998433
},
{
"id": "https://openalex.org/C104317684",
"wikidata": "https://www.wikidata.org/wiki/Q7187",
"display_name": "Gene",
"level": 2,
"score": 0.17674786
},
{
"id": "https://openalex.org/C135763542",
"wikidata": "https://www.wikidata.org/wiki/Q106016",
"display_name": "Genotype",
"level": 3,
"score": 0.1574935
}
],
"corresponding_author_ids": [],
"corresponding_institution_ids": [],
"countries_distinct_count": 1,
"counts_by_year": [
{
"year": 2024,
"cited_by_count": 1
},
{
"year": 2023,
"cited_by_count": 2
},
{
"year": 2021,
"cited_by_count": 1
}
],
"created_date": "2018-08-22",
"datasets": [],
"display_name": "A novel technique for isolating DNA from Tempus\u2122 blood RNA tubes after RNA isolation",
"doi": "https://doi.org/10.1186/s13104-018-3671-4",
"fulltext_origin": "pdf",
"fwci": 0.091,
"grants": [
{
"funder": "https://openalex.org/F4320332183",
"funder_display_name": "U.S. Geological Survey",
"award_id": null
}
],
"has_fulltext": true,
"id": "https://openalex.org/W2886495745",
"ids": {
"openalex": "https://openalex.org/W2886495745",
"doi": "https://doi.org/10.1186/s13104-018-3671-4",
"mag": "2886495745",
"pmid": "https://pubmed.ncbi.nlm.nih.gov/30081941",
"pmcid": "https://www.ncbi.nlm.nih.gov/pmc/articles/6080204"
},
"indexed_in": [
"crossref",
"doaj",
"pubmed"
],
"institutions_distinct_count": 3,
"is_paratext": false,
"is_retracted": false,
"keywords": [
{
"id": "https://openalex.org/keywords/genomic-dna",
"display_name": "genomic DNA",
"score": 0.58024263
},
{
"id": "https://openalex.org/keywords/rna-integrity",
"display_name": "RNA Integrity",
"score": 0.565828
},
{
"id": "https://openalex.org/keywords/rna-silencing",
"display_name": "RNA Silencing",
"score": 0.53208
},
{
"id": "https://openalex.org/keywords/dna-barcoding",
"display_name": "DNA Barcoding",
"score": 0.525103
},
{
"id": "https://openalex.org/keywords/environmental-dna",
"display_name": "Environmental DNA",
"score": 0.522976
},
{
"id": "https://openalex.org/keywords/agarose",
"display_name": "Agarose",
"score": 0.4109301
}
],
"language": "en",
"locations": [
{
"is_oa": true,
"landing_page_url": "https://doi.org/10.1186/s13104-018-3671-4",
"pdf_url": "https://bmcresnotes.biomedcentral.com/track/pdf/10.1186/s13104-018-3671-4",
"source": {
"id": "https://openalex.org/S100014455",
"display_name": "BMC Research Notes",
"issn_l": "1756-0500",
"issn": [
"1756-0500"
],
"is_oa": true,
"is_in_doaj": true,
"is_core": true,
"host_organization": "https://openalex.org/P4310320256",
"host_organization_name": "BioMed Central",
"host_organization_lineage": [
"https://openalex.org/P4310319965",
"https://openalex.org/P4310320256"
],
"host_organization_lineage_names": [
"Springer Nature",
"BioMed Central"
],
"type": "journal"
},
"license": "cc-by",
"license_id": "https://openalex.org/licenses/cc-by",
"version": "publishedVersion",
"is_accepted": true,
"is_published": true
},
{
"is_oa": false,
"landing_page_url": "https://doaj.org/article/430b62901db74bfda4b240caa085c541",
"pdf_url": null,
"source": {
"id": "https://openalex.org/S4306401280",
"display_name": "DOAJ (DOAJ: Directory of Open Access Journals)",
"issn_l": null,
"issn": null,
"is_oa": true,
"is_in_doaj": false,
"is_core": false,
"host_organization": null,
"host_organization_name": null,
"host_organization_lineage": [],
"host_organization_lineage_names": [],
"type": "repository"
},
"license": null,
"license_id": null,
"version": null,
"is_accepted": false,
"is_published": false
},
{
"is_oa": true,
"landing_page_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6080204",
"pdf_url": null,
"source": {
"id": "https://openalex.org/S2764455111",
"display_name": "PubMed Central",
"issn_l": null,
"issn": null,
"is_oa": true,
"is_in_doaj": false,
"is_core": false,
"host_organization": "https://openalex.org/I1299303238",
"host_organization_name": "National Institutes of Health",
"host_organization_lineage": [
"https://openalex.org/I1299303238"
],
"host_organization_lineage_names": [
"National Institutes of Health"
],
"type": "repository"
},
"license": null,
"license_id": null,
"version": "publishedVersion",
"is_accepted": true,
"is_published": true
},
{
"is_oa": false,
"landing_page_url": "https://pubmed.ncbi.nlm.nih.gov/30081941",
"pdf_url": null,
"source": {
"id": "https://openalex.org/S4306525036",
"display_name": "PubMed",
"issn_l": null,
"issn": null,
"is_oa": false,
"is_in_doaj": false,
"is_core": false,
"host_organization": "https://openalex.org/I1299303238",
"host_organization_name": "National Institutes of Health",
"host_organization_lineage": [
"https://openalex.org/I1299303238"
],
"host_organization_lineage_names": [
"National Institutes of Health"
],
"type": "repository"
},
"license": null,
"license_id": null,
"version": null,
"is_accepted": false,
"is_published": false
}
],
"locations_count": 4,
"mesh": [
{
"descriptor_ui": "D004247",
"descriptor_name": "DNA",
"qualifier_ui": "Q000302",
"qualifier_name": "isolation & purification",
"is_major_topic": true
},
{
"descriptor_ui": "D000818",
"descriptor_name": "Animals",
"qualifier_ui": "",
"qualifier_name": null,
"is_major_topic": false
},
{
"descriptor_ui": "D004247",
"descriptor_name": "DNA",
"qualifier_ui": "",
"qualifier_name": null,
"is_major_topic": false
},
{
"descriptor_ui": "D003670",
"descriptor_name": "Deer",
"qualifier_ui": "",
"qualifier_name": null,
"is_major_topic": false
},
{
"descriptor_ui": "D012313",
"descriptor_name": "RNA",
"qualifier_ui": "",
"qualifier_name": null,
"is_major_topic": false
}
],
"ngrams_url": "https://api.openalex.org/works/W2886495745/ngrams",
"open_access": {
"is_oa": true,
"oa_status": "gold",
"oa_url": "https://bmcresnotes.biomedcentral.com/track/pdf/10.1186/s13104-018-3671-4",
"any_repository_has_fulltext": true
},
"primary_location": {
"is_oa": true,
"landing_page_url": "https://doi.org/10.1186/s13104-018-3671-4",
"pdf_url": "https://bmcresnotes.biomedcentral.com/track/pdf/10.1186/s13104-018-3671-4",
"source": {
"id": "https://openalex.org/S100014455",
"display_name": "BMC Research Notes",
"issn_l": "1756-0500",
"issn": [
"1756-0500"
],
"is_oa": true,
"is_in_doaj": true,
"is_core": true,
"host_organization": "https://openalex.org/P4310320256",
"host_organization_name": "BioMed Central",
"host_organization_lineage": [
"https://openalex.org/P4310319965",
"https://openalex.org/P4310320256"
],
"host_organization_lineage_names": [
"Springer Nature",
"BioMed Central"
],
"type": "journal"
},
"license": "cc-by",
"license_id": "https://openalex.org/licenses/cc-by",
"version": "publishedVersion",
"is_accepted": true,
"is_published": true
},
"primary_topic": {
"id": "https://openalex.org/T11970",
"display_name": "Real-Time Polymerase Chain Reaction",
"score": 0.9993,
"subfield": {
"id": "https://openalex.org/subfields/1312",
"display_name": "Molecular Biology"
},
"field": {
"id": "https://openalex.org/fields/13",
"display_name": "Biochemistry, Genetics and Molecular Biology"
},
"domain": {
"id": "https://openalex.org/domains/1",
"display_name": "Life Sciences"
}
},
"publication_date": "2018-08-06",
"publication_year": 2018,
"referenced_works": [
"https://openalex.org/W1996971137",
"https://openalex.org/W2014218915",
"https://openalex.org/W2036479225",
"https://openalex.org/W2039360253",
"https://openalex.org/W2047523135",
"https://openalex.org/W2069059770",
"https://openalex.org/W2080767196",
"https://openalex.org/W2083287570",
"https://openalex.org/W2092799262",
"https://openalex.org/W2099803556",
"https://openalex.org/W2126627407",
"https://openalex.org/W2136309784",
"https://openalex.org/W2172572161",
"https://openalex.org/W2483073678",
"https://openalex.org/W2611203630",
"https://openalex.org/W2613681603",
"https://openalex.org/W2782645298",
"https://openalex.org/W2784855499"
],
"referenced_works_count": 18,
"related_works": [
"https://openalex.org/W46569427",
"https://openalex.org/W4240549537",
"https://openalex.org/W4231299192",
"https://openalex.org/W2416084300",
"https://openalex.org/W2378369506",
"https://openalex.org/W2371520255",
"https://openalex.org/W2353370357",
"https://openalex.org/W2262848750",
"https://openalex.org/W2033636902",
"https://openalex.org/W1583843090"
],
"sustainable_development_goals": [
{
"id": "https://metadata.un.org/sdg/15",
"display_name": "Life on land",
"score": 0.47
}
],
"title": "A novel technique for isolating DNA from Tempus\u2122 blood RNA tubes after RNA isolation",
"topics": [
{
"id": "https://openalex.org/T11970",
"display_name": "Real-Time Polymerase Chain Reaction",
"score": 0.9993,
"subfield": {
"id": "https://openalex.org/subfields/1312",
"display_name": "Molecular Biology"
},
"field": {
"id": "https://openalex.org/fields/13",
"display_name": "Biochemistry, Genetics and Molecular Biology"
},
"domain": {
"id": "https://openalex.org/domains/1",
"display_name": "Life Sciences"
}
},
{
"id": "https://openalex.org/T12640",
"display_name": "Environmental DNA in Biodiversity Monitoring",
"score": 0.9927,
"subfield": {
"id": "https://openalex.org/subfields/2303",
"display_name": "Ecology"
},
"field": {
"id": "https://openalex.org/fields/23",
"display_name": "Environmental Science"
},
"domain": {
"id": "https://openalex.org/domains/3",
"display_name": "Physical Sciences"
}
},
{
"id": "https://openalex.org/T13220",
"display_name": "Mycoviruses in Fungal Symbiosis and Pathogenesis",
"score": 0.9399,
"subfield": {
"id": "https://openalex.org/subfields/1310",
"display_name": "Endocrinology"
},
"field": {
"id": "https://openalex.org/fields/13",
"display_name": "Biochemistry, Genetics and Molecular Biology"
},
"domain": {
"id": "https://openalex.org/domains/1",
"display_name": "Life Sciences"
}
}
],
"type": "article",
"type_crossref": "journal-article",
"updated_date": "2024-08-13T18:30:03.295463",
"versions": []
}
}
