Item talk:Q248018
From geokb
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"name": "Taqman Real-Time PCR Detects Avipoxvirus DNA in Blood of Hawa\u00eci `Amakihi (Hemignathus virens)",
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"abstract": "Background\nAvipoxvirus\u00a0sp. is a significant threat to endemic bird populations on several groups of islands worldwide, including Hawa\u00eci, the Galapagos Islands, and the Canary Islands. Accurate identification and genotyping of\u00a0Avipoxvirus\u00a0is critical to the study of this disease and how it interacts with other pathogens, but currently available methods rely on invasive sampling of pox-like lesions and may be especially harmful in smaller birds.\nMethodology/Principal Findings\nHere, we present a nested TaqMan Real-Time PCR for the detection of the\u00a0Avipoxvirus\u00a04b core protein gene in archived blood samples from Hawaiian birds. The method was successful in amplifying\u00a0Avipoxvirus\u00a0DNA from packed blood cells of one of seven Hawaiian honeycreepers with confirmed\u00a0Avipoxvirus\u00a0infections and 13 of 28 Hawa\u00eci `amakihi (Hemignathus virens) with suspected\u00a0Avipoxvirus\u00a0infections based on the presence of pox-like lesions. Mixed genotype infections have not previously been documented in Hawa\u00eci but were observed in two individuals in this study.\nConclusions/Significance\nWe anticipate that this method will be applicable to other closely related strains of\u00a0Avipoxvirus\u00a0and will become an important and useful tool in global studies of the epidemiology of\u00a0Avipoxvirus.",
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