Item talk:Q237041
From geokb
{
"USGS Publications Warehouse": { "@context": "https://schema.org", "@type": "Article", "additionalType": "Journal Article", "name": "Evidence for free-living Bacteroides in Cladophora along the shores of the Great Lakes", "identifier": [ { "@type": "PropertyValue", "propertyID": "USGS Publications Warehouse IndexID", "value": "70160696", "url": "https://pubs.usgs.gov/publication/70160696" }, { "@type": "PropertyValue", "propertyID": "USGS Publications Warehouse Internal ID", "value": 70160696 }, { "@type": "PropertyValue", "propertyID": "DOI", "value": "10.3354/ame01688", "url": "https://doi.org/10.3354/ame01688" } ], "journal": { "@type": "Periodical", "name": "Aquatic Microbial Ecology", "volumeNumber": "72", "issueNumber": "2" }, "inLanguage": "en", "isPartOf": [ { "@type": "CreativeWorkSeries", "name": "Aquatic Microbial Ecology" } ], "datePublished": "2014", "dateModified": "2021-08-30", "abstract": "Bacteroides is assumed to be restricted to the alimentary canal of animals and humans and is considered to be non-viable in ambient environments. We hypothesized that Bacteroides could persist and replicate within beach-stranded Cladophora glomerata mats in southern Lake Michigan, USA. Mean Bacteroides concentration (per GenBac3 Taqman quantitative PCR assay) during summer 2012 at Jeorse Park Beach was 5.2 log calibrator cell equivalents (CCE) g-1 dry weight (dw), ranging from 3.7 to 6.7. We monitored a single beach-stranded mat for 3 wk; bacterial concentrations increased by 1.6 log CCE g-1 dw and correlated significantly with ambient temperature (p = 0.003). Clonal growth was evident, as observed by >99% nucleotide sequence similarity among clones. In in vitro studies, Bacteroides concentrations increased by 5.5 log CCE g-1 after 7 d (27\u00b0C) in fresh Cladophora collected from rocks. Partial sequencing of the 16S rRNA gene of 36 clones from the incubation experiment showed highly similar genotypes (\u226597% sequence overlap). The closest enteric Bacteroides spp. from the National Center for Biotechnology Information database were only 87 to 91% similar. Genomic similarity, clonality, growth, and persistence collectively suggest that putative, free-living Bacteroides inhabit Cladophora mats of southern Lake Michigan. These findings may have important biological, medical, regulatory, microbial source tracking, and public health implications.", "description": "10 p.", "publisher": { "@type": "Organization", "name": "Inter-Research" }, "author": [ { "@type": "Person", "name": "Nevers, Meredith mnevers@usgs.gov", "givenName": "Meredith", "familyName": "Nevers", "email": "mnevers@usgs.gov", "identifier": { "@type": "PropertyValue", "propertyID": "ORCID", "value": "0000-0001-6963-6734", "url": "https://orcid.org/0000-0001-6963-6734" }, "affiliation": [ { "@type": "Organization", "name": "Great Lakes Science Center", "url": "https://www.usgs.gov/centers/great-lakes-science-center" } ] }, { "@type": "Person", "name": "Shively, Dawn A. dshively@usgs.gov", "givenName": "Dawn A.", "familyName": "Shively", "email": "dshively@usgs.gov", "affiliation": [ { "@type": "Organization", "name": "Great Lakes Science Center", "url": "https://www.usgs.gov/centers/great-lakes-science-center" } ] }, { "@type": "Person", "name": "Przybyla-Kelly, Katarzyna kprzybyla-kelly@usgs.gov", "givenName": "Katarzyna", "familyName": "Przybyla-Kelly", "email": "kprzybyla-kelly@usgs.gov", "affiliation": [ { "@type": "Organization", "name": "Great Lakes Science Center", "url": "https://www.usgs.gov/centers/great-lakes-science-center" } ] }, { "@type": "Person", "name": "Spoljaric, Ashley aspoljaric@usgs.gov", "givenName": "Ashley", "familyName": "Spoljaric", "email": "aspoljaric@usgs.gov", "identifier": { "@type": "PropertyValue", "propertyID": "ORCID", "value": "0000-0001-6262-030X", "url": "https://orcid.org/0000-0001-6262-030X" }, "affiliation": [ { "@type": "Organization", "name": "Great Lakes Science Center", "url": "https://www.usgs.gov/centers/great-lakes-science-center" } ] }, { "@type": "Person", "name": "Byappanahalli, Muruleedhara byappan@usgs.gov", "givenName": "Muruleedhara", "familyName": "Byappanahalli", "email": "byappan@usgs.gov", "identifier": { "@type": "PropertyValue", "propertyID": "ORCID", "value": "0000-0001-5376-597X", "url": "https://orcid.org/0000-0001-5376-597X" }, "affiliation": [ { "@type": "Organization", "name": "Great Lakes Science Center", "url": "https://www.usgs.gov/centers/great-lakes-science-center" } ] }, { "@type": "Person", "name": "Whitman, Richard L. rwhitman@usgs.gov", "givenName": "Richard L.", "familyName": "Whitman", "email": "rwhitman@usgs.gov", "affiliation": [ { "@type": "Organization", "name": "Great Lakes Science Center", "url": "https://www.usgs.gov/centers/great-lakes-science-center" } ] } ], "funder": [ { "@type": "Organization", "name": "Great Lakes Science Center", "url": "https://www.usgs.gov/centers/great-lakes-science-center" } ], "spatialCoverage": [ { "@type": "Place", "additionalType": "country", "name": "United States", "url": "https://geonames.org/4074035" }, { "@type": "Place", "additionalType": "unknown", "name": "southern Lake Michigan" }, { "@type": "Place", "geo": [ { "@type": "GeoShape", "additionalProperty": { "@type": 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