Item talk:Q318756

{

 "id": "10.5066/p1aqghwq",
 "attributes": {
   "doi": "10.5066/p1aqghwq",
   "identifiers": [],
   "creators": [
     {
       "name": "Susan Knowles",
       "nameType": "Personal",
       "affiliation": [
         "United States Geological Survey"
       ],
       "nameIdentifiers": [
         {
           "schemeUri": "https://orcid.org",
           "nameIdentifier": "https://orcid.org/0000-0002-0254-6491",
           "nameIdentifierScheme": "ORCID"
         }
       ]
     },
     {
       "name": "Eric Leis",
       "nameType": "Personal",
       "affiliation": [
         "U.S. Fish and Wildlife Service La Crosse Fish Health Center"
       ],
       "nameIdentifiers": [
         {
           "schemeUri": "https://orcid.org",
           "nameIdentifier": null,
           "nameIdentifierScheme": "ORCID"
         }
       ]
     },
     {
       "name": "Jordan Richard",
       "nameType": "Personal",
       "affiliation": [
         "USFWS-Virginia Field Office"
       ],
       "nameIdentifiers": [
         {
           "schemeUri": "https://orcid.org",
           "nameIdentifier": null,
           "nameIdentifierScheme": "ORCID"
         }
       ]
     },
     {
       "name": "Jamie Bojko",
       "nameType": "Personal",
       "affiliation": [
         "Teesside University"
       ],
       "nameIdentifiers": [
         {
           "schemeUri": "https://orcid.org",
           "nameIdentifier": null,
           "nameIdentifierScheme": "ORCID"
         }
       ]
     },
     {
       "name": "Jesse Weinzinger",
       "nameType": "Personal",
       "affiliation": [
         "WI DNR"
       ],
       "nameIdentifiers": [
         {
           "schemeUri": "https://orcid.org",
           "nameIdentifier": null,
           "nameIdentifierScheme": "ORCID"
         }
       ]
     },
     {
       "name": "Diane L Waller",
       "nameType": "Personal",
       "affiliation": [
         "United States Geological Survey"
       ],
       "nameIdentifiers": [
         {
           "schemeUri": "https://orcid.org",
           "nameIdentifier": "https://orcid.org/0000-0002-6104-810X",
           "nameIdentifierScheme": "ORCID"
         }
       ]
     }
   ],
   "titles": [
     {
       "title": "2018 Embarrass River Microsporidia"
     }
   ],
   "publisher": "U.S. Geological Survey",
   "container": {},
   "publicationYear": 2024,
   "subjects": [
     {
       "subject": "Wildlife Disease"
     }
   ],
   "contributors": [],
   "dates": [],
   "language": null,
   "types": {
     "ris": "DATA",
     "bibtex": "misc",
     "citeproc": "dataset",
     "schemaOrg": "Dataset",
     "resourceType": "Dataset",
     "resourceTypeGeneral": "Dataset"
   },
   "relatedIdentifiers": [],
   "relatedItems": [],
   "sizes": [],
   "formats": [],
   "version": null,
   "rightsList": [],
   "descriptions": [
     {
       "description": "During an epidemiologic survey following a mortality event of freshwater mussels in 2018 in the Embarrass River, Wisconsin, USA, we identified a novel microsporidian parasite in the ovary of mucket (Actinonaias ligamentina), plain pocketbook (Lampsilis cardium), and fatmucket (Lampsilis siliquoidea) (Unioinidae). Histopathology showed round-to-oval microsporidial spores in the cytoplasm of oocytes in 60% (3/5) of mucket, 100% (4/4) of plain pocketbook and 50% (1/2) of fatmucket. On transmission electron microscopy, mature spores were round to oval, measured 4.13 +/- 0.64 \u00b5m (3.14\u20135.31) long by 2.88 +/-0.37 \u00b5m (2.36\u20133.68) wide. Spores had a thin electron dense exospore with a spiky coat, a thick electron lucent endospore, diplokaryotic nuclei, a polar vacuole, and 27\u201328 polar filaments arranged in 1 to 3 rows. Sequencing of the small subunit rRNA produced a 1356 bp sequence most similar to Pseudonosema cristatellae, and phylogenetic analysis grouped it with freshwater Neopereziida. The morphologic and ultrastructural characteristics did not match those of Pseudonosema sp. and a new genus and species, Hirsutonosema embarrassi n. gen., n. sp., were designated. Additional studies could evaluate host susceptibility, distribution, seasonality, transmission, and lethal or sub-lethal effects of this parasite to freshwater mussels.",
       "descriptionType": "Abstract"
     }
   ],
   "geoLocations": [],
   "fundingReferences": [],
   "url": "https://www.sciencebase.gov/catalog/item/660b1df3d34e4df16bd58c39",
   "contentUrl": null,
   "metadataVersion": 1,
   "schemaVersion": "http://datacite.org/schema/kernel-4",
   "source": "api",
   "isActive": true,
   "state": "findable",
   "reason": null,
   "viewCount": 0,
   "downloadCount": 0,
   "referenceCount": 0,
   "citationCount": 0,
   "partCount": 0,
   "partOfCount": 0,
   "versionCount": 0,
   "versionOfCount": 0,
   "created": "2024-06-05T17:13:02Z",
   "registered": "2024-06-05T17:13:02Z",
   "published": null,
   "updated": "2024-06-05T17:13:12Z"
 },
 "relationships": {
   "client": {
     "data": {
       "id": "usgs.prod",
       "type": "clients"
     }
   }
 },
 "type": "dois"

}