Item talk:Q306260

From geokb
Revision as of 19:39, 22 August 2024 by Sky (talk | contribs) (Created page with "{ "USGS Publications Warehouse": { "@context": "https://schema.org", "@type": "Article", "additionalType": "Journal Article", "name": "Inactivation of viable surrogates for the select agents virulent Newcastle disease virus and highly pathogenic avian influenza virus using either commercial lysis buffer or heat", "identifier": [ { "@type": "PropertyValue", "propertyID": "USGS Publications Warehouse IndexID", "value": "7...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

{

 "USGS Publications Warehouse": {
   "@context": "https://schema.org",
   "@type": "Article",
   "additionalType": "Journal Article",
   "name": "Inactivation of viable surrogates for the select agents virulent Newcastle disease virus and highly pathogenic avian influenza virus using either commercial lysis buffer or heat",
   "identifier": [
     {
       "@type": "PropertyValue",
       "propertyID": "USGS Publications Warehouse IndexID",
       "value": "70210921",
       "url": "https://pubs.usgs.gov/publication/70210921"
     },
     {
       "@type": "PropertyValue",
       "propertyID": "USGS Publications Warehouse Internal ID",
       "value": 70210921
     },
     {
       "@type": "PropertyValue",
       "propertyID": "DOI",
       "value": "10.1177/1535676019888920",
       "url": "https://doi.org/10.1177/1535676019888920"
     }
   ],
   "journal": {
     "@type": "Periodical",
     "name": "Applied Biosafety",
     "volumeNumber": "24",
     "issueNumber": "4"
   },
   "inLanguage": "en",
   "isPartOf": [
     {
       "@type": "CreativeWorkSeries",
       "name": "Applied Biosafety"
     }
   ],
   "datePublished": "2019",
   "dateModified": "2020-07-03",
   "abstract": "Introduction:Federal Select Agent Program regulations require laboratories to document a validated procedure for inactivating select agents prior to movement outside registered space. Avian influenza viruses and virulent Newcastle disease virus (vNDV) are cultured in chicken amnio-allantoic fluid (AAF), but the efficacy of commercial lysis buffers to inactivate viruses in protein-rich media has not been documented.Objectives:We assesses the efficacy of MagMAX\u2122 lysis buffer for inactivating highly pathogenic avian influenza virus (HPAIV) and vNDV in chicken AAF and confirm the inactivation of avian influenza in serum using heat.Methods:Low pathogenic avian influenza virus (LPAIV) and avian paramyxovirus subtype-1 (APMV-1) were incubated with lysis buffer and tested for viability. Known viable LPAIV and APMV-1 RNA was extracted from AAF using MagMAX\u2122-96 AI/ND Viral RNA Isolation kit, and the eluate was tested for remaining infectious agent. Finally, inactivation of LPAIV in serum was examined over 3 combinations of temperature and incubation time.Results:MagMAX\u2122 lysis buffer inactivated both LPAIV and APMV-1 in AAF when incubated for 30 minutes at room temperature. The full extraction process eliminated viable virus from the final RNA eluate. LPAIV in serum heated to 70\u00b0C for 30 minutes was rendered noninfectious.Conclusion:The ability of a diagnostic laboratory to move samples from one space to another is critical to maintaining biosecurity as well as efficient laboratory workflow. Our study demonstrates a method to ensure the inactivation of viable avian influenza and avian paramyxoviruses in AAF, RNA eluate, and viable avian influenza virus in sera.",
   "description": "11 p.",
   "publisher": {
     "@type": "Organization",
     "name": "Sage"
   },
   "author": [
     {
       "@type": "Person",
       "name": "Alger, Katrina E.",
       "givenName": "Katrina E.",
       "familyName": "Alger",
       "identifier": {
         "@type": "PropertyValue",
         "propertyID": "ORCID",
         "value": "0000-0001-7708-0203",
         "url": "https://orcid.org/0000-0001-7708-0203"
       },
       "affiliation": [
         {
           "@type": "Organization",
           "name": "National Wildlife Health Center",
           "url": "https://www.usgs.gov/centers/nwhc"
         }
       ]
     },
     {
       "@type": "Person",
       "name": "Ip, Hon S. hip@usgs.gov",
       "givenName": "Hon S.",
       "familyName": "Ip",
       "email": "hip@usgs.gov",
       "identifier": {
         "@type": "PropertyValue",
         "propertyID": "ORCID",
         "value": "0000-0003-4844-7533",
         "url": "https://orcid.org/0000-0003-4844-7533"
       },
       "affiliation": [
         {
           "@type": "Organization",
           "name": "National Wildlife Health Center",
           "url": "https://www.usgs.gov/centers/nwhc"
         }
       ]
     },
     {
       "@type": "Person",
       "name": "Hall, Jeffrey S. jshall@usgs.gov",
       "givenName": "Jeffrey S.",
       "familyName": "Hall",
       "email": "jshall@usgs.gov",
       "identifier": {
         "@type": "PropertyValue",
         "propertyID": "ORCID",
         "value": "0000-0001-5599-2826",
         "url": "https://orcid.org/0000-0001-5599-2826"
       },
       "affiliation": [
         {
           "@type": "Organization",
           "name": "National Wildlife Health Center",
           "url": "https://www.usgs.gov/centers/nwhc"
         }
       ]
     },
     {
       "@type": "Person",
       "name": "Nashold, Sean",
       "givenName": "Sean",
       "familyName": "Nashold",
       "identifier": {
         "@type": "PropertyValue",
         "propertyID": "ORCID",
         "value": "0000-0002-8869-6633",
         "url": "https://orcid.org/0000-0002-8869-6633"
       },
       "affiliation": [
         {
           "@type": "Organization",
           "name": "National Wildlife Health Center",
           "url": "https://www.usgs.gov/centers/nwhc"
         }
       ]
     },
     {
       "@type": "Person",
       "name": "Richgels, Katherine krichgels@usgs.gov",
       "givenName": "Katherine",
       "familyName": "Richgels",
       "email": "krichgels@usgs.gov",
       "identifier": {
         "@type": "PropertyValue",
         "propertyID": "ORCID",
         "value": "0000-0003-2834-9477",
         "url": "https://orcid.org/0000-0003-2834-9477"
       },
       "affiliation": [
         {
           "@type": "Organization",
           "name": "National Wildlife Health Center",
           "url": "https://www.usgs.gov/centers/nwhc"
         }
       ]
     },
     {
       "@type": "Person",
       "name": "Smith, Carrie Alison",
       "givenName": "Carrie Alison",
       "familyName": "Smith",
       "identifier": {
         "@type": "PropertyValue",
         "propertyID": "ORCID",
         "value": "0000-0002-2684-3407",
         "url": "https://orcid.org/0000-0002-2684-3407"
       },
       "affiliation": [
         {
           "@type": "Organization",
           "name": "National Wildlife Health Center",
           "url": "https://www.usgs.gov/centers/nwhc"
         }
       ]
     }
   ],
   "funder": [
     {
       "@type": "Organization",
       "name": "National Wildlife Health Center",
       "url": "https://www.usgs.gov/centers/nwhc"
     }
   ]
 },
 "OpenAlex": {
   "_id": "https://openalex.org/w2996033060",
   "abstract_inverted_index": {
     "Federal": [
       0
     ],
     "Select": [
       1
     ],
     "Agent": [
       2
     ],
     "Program": [
       3
     ],
     "regulations": [
       4
     ],
     "require": [
       5
     ],
     "laboratories": [
       6
     ],
     "to": [
       7,
       17,
       45,
       183,
       197,
       203,
       207,
       221
     ],
     "document": [
       8
     ],
     "a": [
       9,
       194,
       219
     ],
     "validated": [
       10
     ],
     "procedure": [
       11
     ],
     "for": [
       12,
       63,
       105,
       129,
       161,
       185
     ],
     "inactivating": [
       13,
       64
     ],
     "select": [
       14
     ],
     "agents": [
       15
     ],
     "prior": [
       16
     ],
     "movement": [
       18
     ],
     "outside": [
       19
     ],
     "registered": [
       20
     ],
     "space.": [
       21
     ],
     "Avian": [
       22
     ],
     "influenza": [
       23,
       68,
       82,
       90,
       228,
       239
     ],
     "viruses": [
       24,
       47
     ],
     "and": [
       25,
       71,
       76,
       93,
       103,
       110,
       124,
       146,
       155,
       229,
       236
     ],
     "virulent": [
       26
     ],
     "Newcastle": [
       27
     ],
     "disease": [
       28
     ],
     "virus": [
       29,
       69,
       91,
       173,
       240
     ],
     "(vNDV)": [
       30
     ],
     "are": [
       31
     ],
     "cultured": [
       32
     ],
     "in": [
       33,
       48,
       73,
       83,
       137,
       157,
       180,
       232,
       241
     ],
     "chicken": [
       34,
       74
     ],
     "amnio-allantoic": [
       35
     ],
     "fluid": [
       36
     ],
     "(AAF),": [
       37
     ],
     "but": [
       38
     ],
     "the": [
       39,
       57,
       78,
       125,
       175,
       223
     ],
     "efficacy": [
       40,
       58
     ],
     "of": [
       41,
       59,
       80,
       135,
       144,
       193,
       225
     ],
     "commercial": [
       42
     ],
     "lysis": [
       43,
       61,
       101,
       150
     ],
     "buffers": [
       44
     ],
     "inactivate": [
       46
     ],
     "protein-rich": [
       49
     ],
     "media": [
       50
     ],
     "has": [
       51
     ],
     "not": [
       52
     ],
     "been": [
       53
     ],
     "documented.": [
       54
     ],
     "We": [
       55
     ],
     "assesses": [
       56
     ],
     "MagMAX\u2122": [
       60,
       149
     ],
     "buffer": [
       62,
       102,
       151
     ],
     "highly": [
       65
     ],
     "pathogenic": [
       66,
       88
     ],
     "avian": [
       67,
       81,
       89,
       94,
       227,
       230,
       238
     ],
     "(HPAIV)": [
       70
     ],
     "vNDV": [
       72
     ],
     "AAF": [
       75,
       116,
       158
     ],
     "confirm": [
       77
     ],
     "inactivation": [
       79,
       134,
       224
     ],
     "serum": [
       84,
       138,
       181
     ],
     "using": [
       85,
       117
     ],
     "heat.": [
       86
     ],
     "Low": [
       87
     ],
     "(LPAIV)": [
       92
     ],
     "paramyxovirus": [
       95
     ],
     "subtype-1": [
       96
     ],
     "(APMV-1)": [
       97
     ],
     "were": [
       98
     ],
     "incubated": [
       99,
       160
     ],
     "with": [
       100
     ],
     "tested": [
       104,
       128
     ],
     "viability.": [
       106
     ],
     "Known": [
       107
     ],
     "viable": [
       108,
       172,
       226,
       237
     ],
     "LPAIV": [
       109,
       136,
       154,
       179
     ],
     "APMV-1": [
       111,
       156
     ],
     "RNA": [
       112,
       121,
       177,
       234
     ],
     "was": [
       113,
       127,
       139,
       188
     ],
     "extracted": [
       114
     ],
     "from": [
       115,
       174,
       200
     ],
     "MagMAX\u2122-96": [
       118
     ],
     "AI/ND": [
       119
     ],
     "Viral": [
       120
     ],
     "Isolation": [
       122
     ],
     "kit,": [
       123
     ],
     "eluate": [
       126
     ],
     "remaining": [
       130
     ],
     "infectious": [
       131
     ],
     "agent.": [
       132
     ],
     "Finally,": [
       133
     ],
     "examined": [
       140
     ],
     "over": [
       141
     ],
     "3": [
       142
     ],
     "combinations": [
       143
     ],
     "temperature": [
       145
     ],
     "incubation": [
       147
     ],
     "time.": [
       148
     ],
     "inactivated": [
       152
     ],
     "both": [
       153
     ],
     "when": [
       159
     ],
     "30": [
       162,
       186
     ],
     "minutes": [
       163,
       187
     ],
     "at": [
       164
     ],
     "room": [
       165
     ],
     "temperature.": [
       166
     ],
     "The": [
       167,
       191
     ],
     "full": [
       168
     ],
     "extraction": [
       169
     ],
     "process": [
       170
     ],
     "eliminated": [
       171
     ],
     "final": [
       176
     ],
     "eluate.": [
       178
     ],
     "heated": [
       182
     ],
     "70\u00b0C": [
       184
     ],
     "rendered": [
       189
     ],
     "noninfectious.": [
       190
     ],
     "ability": [
       192
     ],
     "diagnostic": [
       195
     ],
     "laboratory": [
       196,
       214
     ],
     "move": [
       198
     ],
     "samples": [
       199
     ],
     "one": [
       201
     ],
     "space": [
       202
     ],
     "another": [
       204
     ],
     "is": [
       205
     ],
     "critical": [
       206
     ],
     "maintaining": [
       208
     ],
     "biosecurity": [
       209
     ],
     "as": [
       210,
       212
     ],
     "well": [
       211
     ],
     "efficient": [
       213
     ],
     "workflow.": [
       215
     ],
     "Our": [
       216
     ],
     "study": [
       217
     ],
     "demonstrates": [
       218
     ],
     "method": [
       220
     ],
     "ensure": [
       222
     ],
     "paramyxoviruses": [
       231
     ],
     "AAF,": [
       233
     ],
     "eluate,": [
       235
     ],
     "sera.": [
       242
     ]
   },
   "apc_list": null,
   "apc_paid": null,
   "authorships": [
     {
       "author_position": "first",
       "author": {
         "id": "https://openalex.org/A5038733162",
         "display_name": "Katrina Alger",
         "orcid": "https://orcid.org/0000-0001-7708-0203"
       },
       "institutions": [
         {
           "id": "https://openalex.org/I4210131920",
           "display_name": "National Wildlife Health Center",
           "ror": "https://ror.org/038d10y34",
           "country_code": "US",
           "type": "healthcare",
           "lineage": [
             "https://openalex.org/I1286329397",
             "https://openalex.org/I1335927249",
             "https://openalex.org/I4210131920"
           ]
         },
         {
           "id": "https://openalex.org/I1286329397",
           "display_name": "United States Geological Survey",
           "ror": "https://ror.org/035a68863",
           "country_code": "US",
           "type": "government",
           "lineage": [
             "https://openalex.org/I1286329397",
             "https://openalex.org/I1335927249"
           ]
         }
       ],
       "countries": [
         "US"
       ],
       "is_corresponding": true,
       "raw_author_name": "Katrina Alger",
       "raw_affiliation_strings": [
         "U.S. Geological Survey National Wildlife Health Center, Madison, WI, USA"
       ],
       "affiliations": [
         {
           "raw_affiliation_string": "U.S. Geological Survey National Wildlife Health Center, Madison, WI, USA",
           "institution_ids": [
             "https://openalex.org/I4210131920",
             "https://openalex.org/I1286329397"
           ]
         }
       ]
     },
     {
       "author_position": "middle",
       "author": {
         "id": "https://openalex.org/A5084311164",
         "display_name": "Hon S. Ip",
         "orcid": "https://orcid.org/0000-0003-4844-7533"
       },
       "institutions": [
         {
           "id": "https://openalex.org/I4210131920",
           "display_name": "National Wildlife Health Center",
           "ror": "https://ror.org/038d10y34",
           "country_code": "US",
           "type": "healthcare",
           "lineage": [
             "https://openalex.org/I1286329397",
             "https://openalex.org/I1335927249",
             "https://openalex.org/I4210131920"
           ]
         },
         {
           "id": "https://openalex.org/I1286329397",
           "display_name": "United States Geological Survey",
           "ror": "https://ror.org/035a68863",
           "country_code": "US",
           "type": "government",
           "lineage": [
             "https://openalex.org/I1286329397",
             "https://openalex.org/I1335927249"
           ]
         }
       ],
       "countries": [
         "US"
       ],
       "is_corresponding": false,
       "raw_author_name": "Hon Ip",
       "raw_affiliation_strings": [
         "U.S. Geological Survey National Wildlife Health Center, Madison, WI, USA"
       ],
       "affiliations": [
         {
           "raw_affiliation_string": "U.S. Geological Survey National Wildlife Health Center, Madison, WI, USA",
           "institution_ids": [
             "https://openalex.org/I4210131920",
             "https://openalex.org/I1286329397"
           ]
         }
       ]
     },
     {
       "author_position": "middle",
       "author": {
         "id": "https://openalex.org/A5008428655",
         "display_name": "Jeffrey S. Hall",
         "orcid": "https://orcid.org/0000-0001-5599-2826"
       },
       "institutions": [
         {
           "id": "https://openalex.org/I4210131920",
           "display_name": "National Wildlife Health Center",
           "ror": "https://ror.org/038d10y34",
           "country_code": "US",
           "type": "healthcare",
           "lineage": [
             "https://openalex.org/I1286329397",
             "https://openalex.org/I1335927249",
             "https://openalex.org/I4210131920"
           ]
         },
         {
           "id": "https://openalex.org/I1286329397",
           "display_name": "United States Geological Survey",
           "ror": "https://ror.org/035a68863",
           "country_code": "US",
           "type": "government",
           "lineage": [
             "https://openalex.org/I1286329397",
             "https://openalex.org/I1335927249"
           ]
         }
       ],
       "countries": [
         "US"
       ],
       "is_corresponding": false,
       "raw_author_name": "Jeffrey Hall",
       "raw_affiliation_strings": [
         "U.S. Geological Survey National Wildlife Health Center, Madison, WI, USA"
       ],
       "affiliations": [
         {
           "raw_affiliation_string": "U.S. Geological Survey National Wildlife Health Center, Madison, WI, USA",
           "institution_ids": [
             "https://openalex.org/I4210131920",
             "https://openalex.org/I1286329397"
           ]
         }
       ]
     },
     {
       "author_position": "middle",
       "author": {
         "id": "https://openalex.org/A5019843147",
         "display_name": "Sean W. Nashold",
         "orcid": null
       },
       "institutions": [
         {
           "id": "https://openalex.org/I4210131920",
           "display_name": "National Wildlife Health Center",
           "ror": "https://ror.org/038d10y34",
           "country_code": "US",
           "type": "healthcare",
           "lineage": [
             "https://openalex.org/I1286329397",
             "https://openalex.org/I1335927249",
             "https://openalex.org/I4210131920"
           ]
         },
         {
           "id": "https://openalex.org/I1286329397",
           "display_name": "United States Geological Survey",
           "ror": "https://ror.org/035a68863",
           "country_code": "US",
           "type": "government",
           "lineage": [
             "https://openalex.org/I1286329397",
             "https://openalex.org/I1335927249"
           ]
         }
       ],
       "countries": [
         "US"
       ],
       "is_corresponding": false,
       "raw_author_name": "Sean Nashold",
       "raw_affiliation_strings": [
         "U.S. Geological Survey National Wildlife Health Center, Madison, WI, USA"
       ],
       "affiliations": [
         {
           "raw_affiliation_string": "U.S. Geological Survey National Wildlife Health Center, Madison, WI, USA",
           "institution_ids": [
             "https://openalex.org/I4210131920",
             "https://openalex.org/I1286329397"
           ]
         }
       ]
     },
     {
       "author_position": "middle",
       "author": {
         "id": "https://openalex.org/A5045006778",
         "display_name": "Katherine L. D. Richgels",
         "orcid": "https://orcid.org/0000-0003-2834-9477"
       },
       "institutions": [
         {
           "id": "https://openalex.org/I4210131920",
           "display_name": "National Wildlife Health Center",
           "ror": "https://ror.org/038d10y34",
           "country_code": "US",
           "type": "healthcare",
           "lineage": [
             "https://openalex.org/I1286329397",
             "https://openalex.org/I1335927249",
             "https://openalex.org/I4210131920"
           ]
         },
         {
           "id": "https://openalex.org/I1286329397",
           "display_name": "United States Geological Survey",
           "ror": "https://ror.org/035a68863",
           "country_code": "US",
           "type": "government",
           "lineage": [
             "https://openalex.org/I1286329397",
             "https://openalex.org/I1335927249"
           ]
         }
       ],
       "countries": [
         "US"
       ],
       "is_corresponding": false,
       "raw_author_name": "Katherine Richgels",
       "raw_affiliation_strings": [
         "U.S. Geological Survey National Wildlife Health Center, Madison, WI, USA"
       ],
       "affiliations": [
         {
           "raw_affiliation_string": "U.S. Geological Survey National Wildlife Health Center, Madison, WI, USA",
           "institution_ids": [
             "https://openalex.org/I4210131920",
             "https://openalex.org/I1286329397"
           ]
         }
       ]
     },
     {
       "author_position": "last",
       "author": {
         "id": "https://openalex.org/A5024209173",
         "display_name": "Carrie A. Smith",
         "orcid": null
       },
       "institutions": [
         {
           "id": "https://openalex.org/I4210131920",
           "display_name": "National Wildlife Health Center",
           "ror": "https://ror.org/038d10y34",
           "country_code": "US",
           "type": "healthcare",
           "lineage": [
             "https://openalex.org/I1286329397",
             "https://openalex.org/I1335927249",
             "https://openalex.org/I4210131920"
           ]
         },
         {
           "id": "https://openalex.org/I1286329397",
           "display_name": "United States Geological Survey",
           "ror": "https://ror.org/035a68863",
           "country_code": "US",
           "type": "government",
           "lineage": [
             "https://openalex.org/I1286329397",
             "https://openalex.org/I1335927249"
           ]
         }
       ],
       "countries": [
         "US"
       ],
       "is_corresponding": false,
       "raw_author_name": "Carrie Smith",
       "raw_affiliation_strings": [
         "U.S. Geological Survey National Wildlife Health Center, Madison, WI, USA"
       ],
       "affiliations": [
         {
           "raw_affiliation_string": "U.S. Geological Survey National Wildlife Health Center, Madison, WI, USA",
           "institution_ids": [
             "https://openalex.org/I4210131920",
             "https://openalex.org/I1286329397"
           ]
         }
       ]
     }
   ],
   "best_oa_location": {
     "is_oa": true,
     "landing_page_url": "https://doi.org/10.1177/1535676019888920",
     "pdf_url": "https://www.liebertpub.com/doi/pdf/10.1177/1535676019888920",
     "source": {
       "id": "https://openalex.org/S2764852704",
       "display_name": "Applied Biosafety",
       "issn_l": "1535-6760",
       "issn": [
         "1535-6760",
         "2470-1246"
       ],
       "is_oa": false,
       "is_in_doaj": false,
       "is_core": true,
       "host_organization": "https://openalex.org/P4310320017",
       "host_organization_name": "SAGE Publishing",
       "host_organization_lineage": [
         "https://openalex.org/P4310320017"
       ],
       "host_organization_lineage_names": [
         "SAGE Publishing"
       ],
       "type": "journal"
     },
     "license": null,
     "license_id": null,
     "version": "publishedVersion",
     "is_accepted": true,
     "is_published": true
   },
   "biblio": {
     "volume": "24",
     "issue": "4",
     "first_page": "189",
     "last_page": "199"
   },
   "citation_normalized_percentile": {
     "value": 0.509038,
     "is_in_top_1_percent": false,
     "is_in_top_10_percent": false
   },
   "cited_by_api_url": "https://api.openalex.org/works?filter=cites:W2996033060",
   "cited_by_count": 1,
   "cited_by_percentile_year": {
     "min": 63,
     "max": 71
   },
   "concepts": [
     {
       "id": "https://openalex.org/C519248777",
       "wikidata": "https://www.wikidata.org/wiki/Q160122",
       "display_name": "Influenza A virus subtype H5N1",
       "level": 3,
       "score": 0.7986802
     },
     {
       "id": "https://openalex.org/C2778116459",
       "wikidata": "https://www.wikidata.org/wiki/Q327082",
       "display_name": "Newcastle disease",
       "level": 3,
       "score": 0.74990046
     },
     {
       "id": "https://openalex.org/C159047783",
       "wikidata": "https://www.wikidata.org/wiki/Q7215",
       "display_name": "Virology",
       "level": 1,
       "score": 0.7296983
     },
     {
       "id": "https://openalex.org/C2522874641",
       "wikidata": "https://www.wikidata.org/wiki/Q808",
       "display_name": "Virus",
       "level": 2,
       "score": 0.69038546
     },
     {
       "id": "https://openalex.org/C57409179",
       "wikidata": "https://www.wikidata.org/wiki/Q1218293",
       "display_name": "Lysis",
       "level": 2,
       "score": 0.6749675
     },
     {
       "id": "https://openalex.org/C89423630",
       "wikidata": "https://www.wikidata.org/wiki/Q7193",
       "display_name": "Microbiology",
       "level": 1,
       "score": 0.62238646
     },
     {
       "id": "https://openalex.org/C86803240",
       "wikidata": "https://www.wikidata.org/wiki/Q420",
       "display_name": "Biology",
       "level": 0,
       "score": 0.5952058
     },
     {
       "id": "https://openalex.org/C60987743",
       "wikidata": "https://www.wikidata.org/wiki/Q1460232",
       "display_name": "Virulence",
       "level": 3,
       "score": 0.5365766
     },
     {
       "id": "https://openalex.org/C134040284",
       "wikidata": "https://www.wikidata.org/wiki/Q1431049",
       "display_name": "Infectious dose",
       "level": 3,
       "score": 0.5219357
     },
     {
       "id": "https://openalex.org/C2777546802",
       "wikidata": "https://www.wikidata.org/wiki/Q834390",
       "display_name": "Influenza A virus",
       "level": 3,
       "score": 0.45821753
     },
     {
       "id": "https://openalex.org/C2993559633",
       "wikidata": "https://www.wikidata.org/wiki/Q43987",
       "display_name": "Avian influenza virus",
       "level": 4,
       "score": 0.43094748
     },
     {
       "id": "https://openalex.org/C104317684",
       "wikidata": "https://www.wikidata.org/wiki/Q7187",
       "display_name": "Gene",
       "level": 2,
       "score": 0.07553285
     },
     {
       "id": "https://openalex.org/C153911025",
       "wikidata": "https://www.wikidata.org/wiki/Q7202",
       "display_name": "Molecular biology",
       "level": 1,
       "score": 0.057645798
     },
     {
       "id": "https://openalex.org/C55493867",
       "wikidata": "https://www.wikidata.org/wiki/Q7094",
       "display_name": "Biochemistry",
       "level": 1,
       "score": 0.0
     }
   ],
   "corresponding_author_ids": [
     "https://openalex.org/A5038733162"
   ],
   "corresponding_institution_ids": [
     "https://openalex.org/I4210131920",
     "https://openalex.org/I1286329397"
   ],
   "countries_distinct_count": 1,
   "counts_by_year": [
     {
       "year": 2023,
       "cited_by_count": 1
     }
   ],
   "created_date": "2019-12-26",
   "datasets": [],
   "display_name": "Inactivation of Viable Surrogates for the Select Agents Virulent Newcastle Disease Virus and Highly Pathogenic Avian Influenza Virus Using Either Commercial Lysis Buffer or Heat",
   "doi": "https://doi.org/10.1177/1535676019888920",
   "fwci": 0.0,
   "grants": [
     {
       "funder": "https://openalex.org/F4320337355",
       "funder_display_name": "National Institute of Allergy and Infectious Diseases",
       "award_id": "HHSN272201400006C"
     }
   ],
   "has_fulltext": false,
   "id": "https://openalex.org/W2996033060",
   "ids": {
     "openalex": "https://openalex.org/W2996033060",
     "doi": "https://doi.org/10.1177/1535676019888920",
     "mag": "2996033060",
     "pmid": "https://pubmed.ncbi.nlm.nih.gov/36032057"
   },
   "indexed_in": [
     "crossref",
     "pubmed"
   ],
   "institutions_distinct_count": 2,
   "is_paratext": false,
   "is_retracted": false,
   "keywords": [
     {
       "id": "https://openalex.org/keywords/newcastle-disease",
       "display_name": "Newcastle disease",
       "score": 0.74990046
     },
     {
       "id": "https://openalex.org/keywords/infectious-dose",
       "display_name": "Infectious dose",
       "score": 0.5219357
     },
     {
       "id": "https://openalex.org/keywords/host-immune-response",
       "display_name": "Host Immune Response",
       "score": 0.438092
     }
   ],
   "language": "en",
   "locations": [
     {
       "is_oa": true,
       "landing_page_url": "https://doi.org/10.1177/1535676019888920",
       "pdf_url": "https://www.liebertpub.com/doi/pdf/10.1177/1535676019888920",
       "source": {
         "id": "https://openalex.org/S2764852704",
         "display_name": "Applied Biosafety",
         "issn_l": "1535-6760",
         "issn": [
           "1535-6760",
           "2470-1246"
         ],
         "is_oa": false,
         "is_in_doaj": false,
         "is_core": true,
         "host_organization": "https://openalex.org/P4310320017",
         "host_organization_name": "SAGE Publishing",
         "host_organization_lineage": [
           "https://openalex.org/P4310320017"
         ],
         "host_organization_lineage_names": [
           "SAGE Publishing"
         ],
         "type": "journal"
       },
       "license": null,
       "license_id": null,
       "version": "publishedVersion",
       "is_accepted": true,
       "is_published": true
     },
     {
       "is_oa": true,
       "landing_page_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134477",
       "pdf_url": null,
       "source": {
         "id": "https://openalex.org/S2764455111",
         "display_name": "PubMed Central",
         "issn_l": null,
         "issn": null,
         "is_oa": true,
         "is_in_doaj": false,
         "is_core": false,
         "host_organization": "https://openalex.org/I1299303238",
         "host_organization_name": "National Institutes of Health",
         "host_organization_lineage": [
           "https://openalex.org/I1299303238"
         ],
         "host_organization_lineage_names": [
           "National Institutes of Health"
         ],
         "type": "repository"
       },
       "license": null,
       "license_id": null,
       "version": "publishedVersion",
       "is_accepted": true,
       "is_published": true
     },
     {
       "is_oa": false,
       "landing_page_url": "https://pubmed.ncbi.nlm.nih.gov/36032057",
       "pdf_url": null,
       "source": {
         "id": "https://openalex.org/S4306525036",
         "display_name": "PubMed",
         "issn_l": null,
         "issn": null,
         "is_oa": false,
         "is_in_doaj": false,
         "is_core": false,
         "host_organization": "https://openalex.org/I1299303238",
         "host_organization_name": "National Institutes of Health",
         "host_organization_lineage": [
           "https://openalex.org/I1299303238"
         ],
         "host_organization_lineage_names": [
           "National Institutes of Health"
         ],
         "type": "repository"
       },
       "license": null,
       "license_id": null,
       "version": null,
       "is_accepted": false,
       "is_published": false
     }
   ],
   "locations_count": 3,
   "mesh": [],
   "ngrams_url": "https://api.openalex.org/works/W2996033060/ngrams",
   "open_access": {
     "is_oa": true,
     "oa_status": "bronze",
     "oa_url": "https://www.liebertpub.com/doi/pdf/10.1177/1535676019888920",
     "any_repository_has_fulltext": true
   },
   "primary_location": {
     "is_oa": true,
     "landing_page_url": "https://doi.org/10.1177/1535676019888920",
     "pdf_url": "https://www.liebertpub.com/doi/pdf/10.1177/1535676019888920",
     "source": {
       "id": "https://openalex.org/S2764852704",
       "display_name": "Applied Biosafety",
       "issn_l": "1535-6760",
       "issn": [
         "1535-6760",
         "2470-1246"
       ],
       "is_oa": false,
       "is_in_doaj": false,
       "is_core": true,
       "host_organization": "https://openalex.org/P4310320017",
       "host_organization_name": "SAGE Publishing",
       "host_organization_lineage": [
         "https://openalex.org/P4310320017"
       ],
       "host_organization_lineage_names": [
         "SAGE Publishing"
       ],
       "type": "journal"
     },
     "license": null,
     "license_id": null,
     "version": "publishedVersion",
     "is_accepted": true,
     "is_published": true
   },
   "primary_topic": {
     "id": "https://openalex.org/T10167",
     "display_name": "Influenza Virus Research and Epidemiology",
     "score": 0.9999,
     "subfield": {
       "id": "https://openalex.org/subfields/2713",
       "display_name": "Epidemiology"
     },
     "field": {
       "id": "https://openalex.org/fields/27",
       "display_name": "Medicine"
     },
     "domain": {
       "id": "https://openalex.org/domains/4",
       "display_name": "Health Sciences"
     }
   },
   "publication_date": "2019-12-01",
   "publication_year": 2019,
   "referenced_works": [
     "https://openalex.org/W100265738",
     "https://openalex.org/W126601421",
     "https://openalex.org/W1526440562",
     "https://openalex.org/W2005033572",
     "https://openalex.org/W2050285345",
     "https://openalex.org/W2056055880",
     "https://openalex.org/W2060816683",
     "https://openalex.org/W2085130901",
     "https://openalex.org/W2144309895",
     "https://openalex.org/W2306596008",
     "https://openalex.org/W2607138621",
     "https://openalex.org/W4320801076"
   ],
   "referenced_works_count": 12,
   "related_works": [
     "https://openalex.org/W2999601022",
     "https://openalex.org/W2552320299",
     "https://openalex.org/W2352678222",
     "https://openalex.org/W2350899495",
     "https://openalex.org/W2347644781",
     "https://openalex.org/W2285562173",
     "https://openalex.org/W2055149792",
     "https://openalex.org/W1995527227",
     "https://openalex.org/W1553543121",
     "https://openalex.org/W1504424866"
   ],
   "sustainable_development_goals": [
     {
       "display_name": "Good health and well-being",
       "id": "https://metadata.un.org/sdg/3",
       "score": 0.51
     }
   ],
   "title": "Inactivation of Viable Surrogates for the Select Agents Virulent Newcastle Disease Virus and Highly Pathogenic Avian Influenza Virus Using Either Commercial Lysis Buffer or Heat",
   "topics": [
     {
       "id": "https://openalex.org/T10167",
       "display_name": "Influenza Virus Research and Epidemiology",
       "score": 0.9999,
       "subfield": {
         "id": "https://openalex.org/subfields/2713",
         "display_name": "Epidemiology"
       },
       "field": {
         "id": "https://openalex.org/fields/27",
         "display_name": "Medicine"
       },
       "domain": {
         "id": "https://openalex.org/domains/4",
         "display_name": "Health Sciences"
       }
     },
     {
       "id": "https://openalex.org/T11560",
       "display_name": "Dynamics of Livestock Disease Transmission and Control",
       "score": 0.9997,
       "subfield": {
         "id": "https://openalex.org/subfields/1102",
         "display_name": "Agronomy and Crop Science"
       },
       "field": {
         "id": "https://openalex.org/fields/11",
         "display_name": "Agricultural and Biological Sciences"
       },
       "domain": {
         "id": "https://openalex.org/domains/1",
         "display_name": "Life Sciences"
       }
     },
     {
       "id": "https://openalex.org/T11243",
       "display_name": "Epidemiology and Pathogenesis of Respiratory Viral Infections",
       "score": 0.996,
       "subfield": {
         "id": "https://openalex.org/subfields/2713",
         "display_name": "Epidemiology"
       },
       "field": {
         "id": "https://openalex.org/fields/27",
         "display_name": "Medicine"
       },
       "domain": {
         "id": "https://openalex.org/domains/4",
         "display_name": "Health Sciences"
       }
     }
   ],
   "type": "article",
   "type_crossref": "journal-article",
   "updated_date": "2024-08-14T15:13:42.461909",
   "versions": []
 }

}