Item talk:Q261304

{

 "USGS Publications Warehouse": {
   "@context": "https://schema.org",
   "@type": "Article",
   "additionalType": "Journal Article",
   "name": "Correlation of transforming growth factor-\u03b2 messenger RNA (TGF-\u03b2 mRNA) expression with cellular immunoassays in Triamcinolone-treated captive hybrid striped bass",
   "identifier": [
     {
       "@type": "PropertyValue",
       "propertyID": "USGS Publications Warehouse IndexID",
       "value": "1015022",
       "url": "https://pubs.usgs.gov/publication/1015022"
     },
     {
       "@type": "PropertyValue",
       "propertyID": "USGS Publications Warehouse Internal ID",
       "value": 1015022
     },
     {
       "@type": "PropertyValue",
       "propertyID": "DOI",
       "value": "10.1577/1548-8667(2000)012<0009:COTGFM>2.0.CO;2",
       "url": "https://doi.org/10.1577/1548-8667(2000)012<0009:COTGFM>2.0.CO;2"
     }
   ],
   "journal": {
     "@type": "Periodical",
     "name": "Journal of Aquatic Animal Health",
     "volumeNumber": "12",
     "issueNumber": "1"
   },
   "inLanguage": "en",
   "isPartOf": [
     {
       "@type": "CreativeWorkSeries",
       "name": "Journal of Aquatic Animal Health"
     }
   ],
   "datePublished": "2000",
   "dateModified": "2020-09-01",
   "abstract": "Assessing fish immune status with molecular markers has been hampered by a lack of specific reagents. A quantitative polymerase chain reaction (PCR) method (reverse transcription quantitative\u2013competitive PCR, RT-qcPCR) for measuring transforming growth factor-\u03b2 (TGF-\u03b2) transcription from a broad range of teleost fish has recently been developed. The quantitative PCR now permits monitoring production of this important immunosuppressive cytokine in response to immunomodulating agents and conditions. We examined anterior kidney and spleen mononuclear cells from hybrid striped bass (female striped bass Morone saxatilis\u00d7 male white bass M. chrysops) for production of TGF-\u03b2 messenger RNA (mRNA) in response to administration of the synthetic glucocorticoid triamcinolone. We also compared TGF-\u03b2 transcription with anterior kidney macrophage bactericidal activity and splenic lymphocyte blastogenesis. Anterior kidney mononuclear cell TGF-\u03b2 mRNA levels decreased, whereas bactericidal activity increased. Spleen TGF-\u03b2 mRNA levels did not change significantly, and splenic lymphocyte pokeweed mitogen stimulation index increased in triamcinolone-treated fish. Since triamcinolone is used therapeutically as a suppressive immunomodulator, the enhanced immune functions indicated by the cellular immunoassays were unexpected; however, the inverse response of TGF-\u03b2 production and macrophage bactericidal activity was consistent with the known relationship between TGF-\u03b2 and macrophage activation in mammals. Induced immunomodulation in hybrid striped bass was detectable by both traditional cellular immunoassays and the new RT-qcPCR for TGF-\u03b2.",
   "description": "9 p.",
   "publisher": {
     "@type": "Organization",
     "name": "Taylor & Francis"
   },
   "author": [
     {
       "@type": "Person",
       "name": "Ottinger, Christopher A. cottinger@usgs.gov",
       "givenName": "Christopher A.",
       "familyName": "Ottinger",
       "email": "cottinger@usgs.gov",
       "identifier": {
         "@type": "PropertyValue",
         "propertyID": "ORCID",
         "value": "0000-0003-2551-1985",
         "url": "https://orcid.org/0000-0003-2551-1985"
       },
       "affiliation": [
         {
           "@type": "Organization",
           "name": "Leetown Science Center",
           "url": "https://www.usgs.gov/centers/eesc"
         }
       ]
     },
     {
       "@type": "Person",
       "name": "Kennedy-Stoskopf, Suzanne",
       "givenName": "Suzanne",
       "familyName": "Kennedy-Stoskopf"
     },
     {
       "@type": "Person",
       "name": "Harms, Craig A.",
       "givenName": "Craig A.",
       "familyName": "Harms"
     }
   ],
   "funder": [
     {
       "@type": "Organization",
       "name": "Leetown Science Center",
       "url": "https://www.usgs.gov/centers/eesc"
     }
   ]
 }

}