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Using the EPA Level III ecoregions as the geographic framework, scientists process geospatial data collected between 1973 and 2000 to characterize ecosystem responses to land-use changes. The 27-year study period was divided into five temporal periods: 1973-1980, 1980-1986, 1986-1992, 1992-2000 and 1973-2000. General land-cover classes for these periods were interpreted from Landsat Multispectral Scanner, Thematic Mapper, and Enhanced Thematic Mapper Plus imagery to categorize land-cover change and evaluate using a modified Anderson Land Use Land Cover Classification System for image interpretation.\r\n\r\nThe rates of land-cover change are estimated using a stratified, random sampling of 10-kilometer (km) by 10-km blocks allocated within each ecoregion. For each sample block, satellite images are used to interpret land-cover change. Additionally, historical aerial photographs from similar timeframes and other ancillary data such as census statistics and published literature are used. The sample block data are then incorporated into statistical analyses to generate an overall change matrix for the ecoregion. These change statistics are applicable for different levels of scale, including total change for the individual sample blocks and change estimates for the entire ecoregion. 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