{
"DOI": { "doi": "10.5066/f77p8xnd", "prefix": "10.5066", "suffix": "f77p8xnd", "identifiers": [ { "identifier": "https://doi.org/10.5066/f77p8xnd", "identifierType": "DOI" } ], "alternateIdentifiers": [ { "alternateIdentifierType": "DOI", "alternateIdentifier": "https://doi.org/10.5066/f77p8xnd" } ], "creators": [ { "name": "Byappanahalli, Muruleedhara", "nameType": "Personal", "givenName": "Muruleedhara", "familyName": "Byappanahalli", "affiliation": [], "nameIdentifiers": [ { "schemeUri": "https://orcid.org", "nameIdentifier": "https://orcid.org/0000-0001-5376-597X", "nameIdentifierScheme": "ORCID" } ] } ], "titles": [ { "title": "Quantitative polymerase chain reaction (qPCR): An alternative, rapid water quality monitoring tool at a National Park on Lake Michigan." } ], "publisher": "U.S. Geological Survey", "container": {}, "publicationYear": 2018, "subjects": [], "contributors": [], "dates": [ { "date": "2014", "dateType": "Collected" }, { "date": "2018", "dateType": "Issued" } ], "language": null, "types": { "ris": "DATA", "bibtex": "misc", "citeproc": "dataset", "schemaOrg": "Dataset", "resourceType": "Dataset", "resourceTypeGeneral": "Dataset" }, "relatedIdentifiers": [ { "relationType": "IsCitedBy", "relatedIdentifier": "https://doi.org/10.2134/jeq2017.11.0462", "relatedIdentifierType": "DOI" } ], "relatedItems": [], "sizes": [], "formats": [ "csv" ], "version": null, "rightsList": [], "descriptions": [ { "description": "Data were collected to evaluate the efficacy of quantitative polymerase chain reaction (qPCR) as a rapid, alternative method for monitoring recreational water at select beaches and rivers of Sleeping Bear Dunes National Lakeshore (SLBE), in Empire, Michigan. Water samples were collected between August 4 and September 18, 2014 (N=297) from four locations (Esch Rd, Otter Creek, Platte Bay, and Platte River). The samples were analyzed for indicator bacteria, E. coli and enterococci, by both culture-based (membrane filtration, MF; traditional method) and non-culture based (qPCR; rapid method). Recreational water quality standards (RWQS) and beach action values (BAV) were used as indices to compare water quality standard exceedances for MF and qPCR methods; for enterococci: 70 colony-forming units (MF) and 1,000 calibrator cell equivalents and for E. coli: 235 colony-forming units (MF); currently, there are no established RWQS based for qPCR (i.e., CCE) results.", "descriptionType": "Abstract" } ], "geoLocations": [], "fundingReferences": [], "xml": "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", "url": "https://www.sciencebase.gov/catalog/item/5a4cf831e4b0d05ee8c4cae0", "contentUrl": null, "metadataVersion": 1, "schemaVersion": null, "source": "mds", "isActive": true, "state": "findable", "reason": null, "viewCount": 0, "viewsOverTime": [], "downloadCount": 0, "downloadsOverTime": [], "referenceCount": 0, "citationCount": 0, "citationsOverTime": [], "partCount": 0, "partOfCount": 0, "versionCount": 0, "versionOfCount": 0, "created": "2018-05-16T13:02:23.000Z", "registered": "2018-05-16T13:02:23.000Z", "published": "2018", "updated": "2020-07-15T17:58:28.000Z" }
}