{
"DOI": {
"doi": "10.5066/p9ru1rvn",
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"creators": [
{
"name": "Damian M Menning",
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"United States Geological Survey"
],
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"schemeUri": "https://orcid.org",
"nameIdentifier": "https://orcid.org/0000-0003-3547-3062",
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},
{
"name": "Barbara J Pierson",
"nameType": "Personal",
"affiliation": [
"United States Geological Survey"
],
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{
"schemeUri": "https://orcid.org",
"nameIdentifier": "https://orcid.org/0000-0001-8233-874X",
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},
{
"name": "Sandra L Talbot",
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"United States Geological Survey"
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],
"titles": [
{
"title": "Detection of Multiple Fish Species Using Environmental DNA (eDNA), Alaska 2018"
}
],
"publisher": "U.S. Geological Survey",
"container": {},
"publicationYear": 2022,
"subjects": [
{
"subject": "Animals/Vertebrates"
},
{
"subject": "DNA"
},
{
"subject": "DNA Primers"
},
{
"subject": "DNA, Mitochondrial"
},
{
"subject": "Fish"
},
{
"subject": "Alaska"
},
{
"subject": "Biodiversity"
},
{
"subject": "Mitochondrial DNA"
},
{
"subject": "Environmental DNA"
},
{
"subject": "Aquatic Ecosystems"
},
{
"subject": "Genetic Diversity"
},
{
"subject": "Species composition"
},
{
"subject": "Ecosystem Diversity"
},
{
"subject": "Native Species"
},
{
"subject": "Animals"
}
],
"contributors": [],
"dates": [
{
"date": "2022-12-13",
"dateType": "Available"
},
{
"date": "2016/2018",
"dateType": "Collected"
}
],
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"rights": "Creative Commons Zero v1.0 Universal",
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"schemeUri": "https://spdx.org/licenses/",
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],
"descriptions": [
{
"description": "This release provides results of an environmental DNA (eDNA) study of fish identified in seven Alaskan lakes, three streams, and a positive control. Samples included replicates for multiple sampling dates and locations. The reference list and conserved primer sets to identify species present were developed using publicly available data (https://www.ncbi.nlm.nih.gov/genbank/). Highly conserved primers which could differentiate species of interest were developed for three portions of mtDNA genes (12S, 16S and COI).",
"descriptionType": "Abstract"
}
],
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"created": "2022-12-13T23:07:23Z",
"registered": "2022-12-13T23:07:24Z",
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"updated": "2024-08-01T22:41:13Z"
}
}