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Supercritical fluid carbon dioxide modified with methanol effectively extracts underivatized methylmercury from certified reference materials Dorm-1 (dogfish muscle) and Dolt-2 (dogfish liver). Calcium chloride and water, with a ratio of 5:2 (by weight), provide the acid environment required for extracting methylmercury from sample matrices. Methylmercury chloride is separated from other organomercury chloride compounds using HPLC. The acidic eluent, containing 0.06 mol L-1 NaCl, insures the presence of methylmercury chloride and facilitates the reduction of mercury on a glassy carbon electrode. If dual glassy carbon electrodes are used, a positive peak is observed at -0.65 to -0.70 V and a negative peak is observed at -0.90V with the organomercury compounds that were tested. The practical detection limit for methylmercury is 5 X 10-8 mol L-1 (1 X 10-12 tool injected) when a 20 ??L injection loop is used.", "description": "18 p.", "publisher": { "@type": "Organization", "name": "Taylor & Francis" }, "author": [ { "@type": "Person", "name": "Simon, N.S.", "givenName": "N.S.", "familyName": "Simon" } ] }, "OpenAlex": { "_id": "https://openalex.org/w2067325472", "abstract_inverted_index": { "Abstract": [ 0 ], "Using": [ 1 ], "the": [ 2, 68, 98, 105, 140 ], "coupled": [ 3 ], "methods": [ 4 ], "presented": [ 5 ], "in": [ 6 ], "this": [ 7 ], "paper,": [ 8 ], "methylmercury": [ 9, 44, 74, 101, 151 ], "can": [ 10 ], "be": [ 11 ], "accurately": [ 12 ], "and": [ 13, 25, 52, 58, 103, 131 ], "rapidly": [ 14 ], "extracted": [ 15 ], "from": [ 16, 45, 75, 82 ], "biological": [ 17 ], "samples": [ 18, 179 ], "by": [ 19 ], "modified": [ 20, 38 ], "supercritical": [ 21 ], "fluid": [ 22, 35, 174 ], "carbon": [ 23, 36, 112, 117 ], "dioxide": [ 24, 37 ], "quantitated": [ 26 ], "using": [ 27, 87 ], "liquid": [ 28 ], "chromatography": [ 29 ], "with": [ 30, 39, 60, 139 ], "reductive": [ 31 ], "electrochemical": [ 32 ], "detection.": [ 33 ], "Supercritical": [ 34 ], "methanol": [ 40 ], "effectively": [ 41 ], "extracts": [ 42 ], "underivatized": [ 43 ], "certified": [ 46 ], "reference": [ 47 ], "materials": [ 48 ], "Dorm-1": [ 49 ], "(dogfish": [ 50, 54 ], "muscle)": [ 51 ], "Dolt-2": [ 53 ], "liver).": [ 55 ], "Calcium": [ 56 ], "chloride": [ 57, 79, 85, 102 ], "water,": [ 59 ], "a": [ 61, 110, 121, 132, 164 ], "ratio": [ 62 ], "of": [ 63, 100, 107 ], "5:2": [ 64 ], "(by": [ 65 ], "weight),": [ 66 ], "provide": [ 67 ], "acid": [ 69 ], "environment": [ 70 ], "required": [ 71 ], "for": [ 72, 150 ], "extracting": [ 73 ], "sample": [ 76 ], "matrices.": [ 77 ], "Methylmercury": [ 78 ], "is": [ 80, 124, 135, 152, 169 ], "separated": [ 81 ], "other": [ 83 ], "organomercury": [ 84, 141 ], "compounds": [ 86, 142 ], "HPLC.": [ 88 ], "The": [ 89, 146 ], "acidic": [ 90 ], "eluent,": [ 91 ], "containing": [ 92 ], "0.06": [ 93 ], "mol": [ 94, 156, 161 ], "L\u20131": [ 95, 157 ], "NaCl,": [ 96 ], "insures": [ 97 ], "presence": [ 99 ], "facilitates": [ 104 ], "reduction": [ 106 ], "mercury": [ 108 ], "on": [ 109 ], "glassy": [ 111, 116 ], "electrode.": [ 113 ], "If": [ 114 ], "dual": [ 115 ], "electrodes": [ 118 ], "are": [ 119 ], "used,": [ 120 ], "positive": [ 122 ], "peak": [ 123, 134 ], "observed": [ 125, 136 ], "at": [ 126, 137 ], "\u20130.65": [ 127 ], "to": [ 128 ], "\u20130.70": [ 129 ], "V": [ 130 ], "negative": [ 133 ], "\u20130.90V": [ 138 ], "that": [ 143 ], "were": [ 144 ], "tested.": [ 145 ], "practical": [ 147 ], "detection": [ 148 ], "limit": [ 149 ], "5": [ 153 ], "\u00d7": [ 154, 159 ], "10\u20138": [ 155 ], "(1": [ 158 ], "10\u201312": [ 160 ], "injected)": [ 162 ], "when": [ 163 ], "20": [ 165 ], "\u03bcL": [ 166 ], "injection": [ 167 ], "loop": [ 168 ], "used.": [ 170 ], "Key": [ 171 ], "Words:": [ 172 ], "Methylmercurysupercritical": [ 173 ], "extraction": [ 175 ], "(SFE)liquid": [ 176 ], "chromatographyelectrochemical": [ 177 ], "detectionbiological": [ 178 ] }, "apc_list": null, "apc_paid": null, "authorships": [ { "author_position": "first", "author": { "id": "https://openalex.org/A5013445180", "display_name": "Nancy S. 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