{
"USGS Publications Warehouse": {
"@context": "https://schema.org",
"@type": "Article",
"additionalType": "Journal Article",
"name": "Assessment of gamete quality for the eastern oyster (Crassostrea virginica) by use of fluorescent dyes",
"identifier": [
{
"@type": "PropertyValue",
"propertyID": "USGS Publications Warehouse IndexID",
"value": "70028482",
"url": "https://pubs.usgs.gov/publication/70028482"
},
{
"@type": "PropertyValue",
"propertyID": "USGS Publications Warehouse Internal ID",
"value": 70028482
},
{
"@type": "PropertyValue",
"propertyID": "DOI",
"value": "10.1016/j.cryobiol.2006.05.001",
"url": "https://doi.org/10.1016/j.cryobiol.2006.05.001"
},
{
"@type": "PropertyValue",
"propertyID": "ISSN",
"value": "00112240"
}
],
"journal": {
"@type": "Periodical",
"name": "Cryobiology",
"volumeNumber": "53",
"issueNumber": "1"
},
"inLanguage": "en",
"isPartOf": [
{
"@type": "CreativeWorkSeries",
"name": "Cryobiology"
}
],
"datePublished": "2006",
"dateModified": "2019-07-26",
"abstract": "Evaluation of sperm motility is the single most widely used parameter to determine semen quality in mammals and aquatic species. While a good indicator for fresh sperm viability, post-thaw motility is not always effective at predicting fertilizing ability. Techniques using fluorescent dyes can assess functionality of mammalian sperm, but have not been widely applied in aquatic organisms. The eastern oyster Crassostrea virginica is an important mollusk in the United States, and cryopreservation protocols have been developed to preserve sperm and larvae to assist research and hatchery production. In this study, protocols were developed to assess sperm cell membrane integrity and mitochondrial function by flow cytometry and to assess viability of eggs by fluorescence microscopy. The fluorescent dyes SYBR 14 and propidium iodide (PI) (to assess membrane integrity) and rhodamine 123 (R123) (to assess mitochondrial membrane potential) were used to evaluate the quality of thawed oyster sperm previously cryopreserved with different cryoprotectant and thawing treatments. Membrane integrity results were correlated with motility of thawed sperm and mitochondrial membrane potential with fertilizing ability. Fluorescein diacetate (FDA) was used to assess cytotoxicity of cryoprotectant solutions and post-thaw damage to oyster eggs. The results indicated that membrane integrity (P = 0.004) and thawing treatments (P = 0.04), and mitochondrial membrane potential (P = 0.0015) were correlated with motility. Fertilizing ability was correlated with cryoprotectant treatments (P = 0.0258) and with mitochondrial membrane potential (P = 0.001). The dye FDA was useful in indicating structural integrity of fresh and thawed eggs. Exposure of eggs, without freezing, to dimethyl sulfoxide yielded higher percentages of stained eggs and fertilization rate than did exposure to propylene glycol (P = 0.002). Thawed eggs were not stained with FDA (<1%) and larvae were not produced.\u00a0",
"description": "11 p.",
"publisher": {
"@type": "Organization",
"name": "Elsevier"
},
"author": [
{
"@type": "Person",
"name": "Tiersch, T.R.",
"givenName": "T.R.",
"familyName": "Tiersch"
},
{
"@type": "Person",
"name": "Paniagua-Chavez, C. G.",
"givenName": "C. G.",
"familyName": "Paniagua-Chavez"
},
{
"@type": "Person",
"name": "Segovia, M.",
"givenName": "M.",
"familyName": "Segovia"
},
{
"@type": "Person",
"name": "Jenkins, J.",
"givenName": "J.",
"familyName": "Jenkins",
"identifier": {
"@type": "PropertyValue",
"propertyID": "ORCID",
"value": "0000-0002-5087-0894",
"url": "https://orcid.org/0000-0002-5087-0894"
}
}
]
},
"OpenAlex": {
"abstract_inverted_index": {
"Evaluation": [
0
],
"of": [
1,
45,
109,
142,
161,
179,
231,
237,
247
],
"sperm": [
2,
26,
78,
95,
145,
163
],
"motility": [
3,
29,
160
],
"is": [
4,
30,
62
],
"the": [
5,
67,
140
],
"single": [
6
],
"most": [
7
],
"widely": [
8,
52
],
"used": [
9,
137,
175
],
"parameter": [
10
],
"to": [
11,
76,
81,
93,
106,
138,
176,
185,
241,
256
],
"determine": [
12
],
"semen": [
13
],
"quality": [
14,
141
],
"in": [
15,
54,
66,
227
],
"mammals": [
16
],
"and": [
17,
70,
79,
84,
99,
105,
119,
127,
151,
164,
182,
195,
199,
216,
233,
250,
268
],
"aquatic": [
18,
55
],
"species.": [
19
],
"While": [
20
],
"a": [
21
],
"good": [
22
],
"indicator": [
23
],
"for": [
24
],
"fresh": [
25,
232
],
"viability,": [
27
],
"post-thaw": [
28,
183
],
"not": [
31,
50,
263,
271
],
"always": [
32
],
"effective": [
33
],
"at": [
34
],
"predicting": [
35
],
"fertilizing": [
36,
169
],
"ability.": [
37,
170
],
"Techniques": [
38
],
"using": [
39
],
"fluorescent": [
40,
115
],
"dyes": [
41,
116
],
"can": [
42
],
"assess": [
43,
94,
107,
124,
132,
177
],
"functionality": [
44
],
"mammalian": [
46
],
"sperm,": [
47
],
"but": [
48
],
"have": [
49,
73
],
"been": [
51,
74
],
"applied": [
53
],
"organisms.": [
56
],
"The": [
57,
114,
188,
222
],
"eastern": [
58
],
"oyster": [
59,
144,
186
],
"Crassostrea": [
60
],
"virginica": [
61
],
"an": [
63
],
"important": [
64
],
"mollusk": [
65
],
"United": [
68
],
"States,": [
69
],
"cryopreservation": [
71
],
"protocols": [
72,
90
],
"developed": [
75,
92
],
"preserve": [
77
],
"larvae": [
80,
269
],
"assist": [
82
],
"research": [
83
],
"hatchery": [
85
],
"production.": [
86
],
"In": [
87
],
"this": [
88
],
"study,": [
89
],
"were": [
91,
136,
157,
204,
262,
270
],
"cell": [
96
],
"membrane": [
97,
125,
134,
166,
192,
201,
219
],
"integrity": [
98,
155,
193,
230
],
"mitochondrial": [
100,
133,
165,
200,
218
],
"function": [
101
],
"by": [
102,
111
],
"flow": [
103
],
"cytometry": [
104
],
"viability": [
108
],
"eggs": [
110,
249,
261
],
"fluorescence": [
112
],
"microscopy.": [
113
],
"SYBR": [
117
],
"14": [
118
],
"propidium": [
120
],
"iodide": [
121
],
"(PI)": [
122
],
"(to": [
123,
131
],
"integrity)": [
126
],
"rhodamine": [
128
],
"123": [
129
],
"(R123)": [
130
],
"potential)": [
135
],
"evaluate": [
139
],
"thawed": [
143,
162,
234
],
"previously": [
146
],
"cryopreserved": [
147
],
"with": [
148,
159,
168,
206,
212,
217,
265
],
"different": [
149
],
"cryoprotectant": [
150,
180,
213
],
"thawing": [
152,
196
],
"treatments.": [
153
],
"Membrane": [
154
],
"results": [
156,
189
],
"correlated": [
158,
205,
211
],
"potential": [
167,
202,
220
],
"Fluorescein": [
171
],
"diacetate": [
172
],
"(FDA)": [
173
],
"was": [
174,
210,
225
],
"cytotoxicity": [
178
],
"solutions": [
181
],
"damage": [
184
],
"eggs.": [
187,
235
],
"indicated": [
190
],
"that": [
191
],
"(P=0.004)": [
194
],
"treatments": [
197,
214
],
"(P=0.04),": [
198
],
"(P=0.0015)": [
203
],
"motility.": [
207
],
"Fertilizing": [
208
],
"ability": [
209
],
"(P=0.0258)": [
215
],
"(P=0.001).": [
221
],
"dye": [
223
],
"FDA": [
224,
266
],
"useful": [
226
],
"indicating": [
228
],
"structural": [
229
],
"Exposure": [
236
],
"eggs,": [
238
],
"without": [
239
],
"freezing,": [
240
],
"dimethyl": [
242
],
"sulfoxide": [
243
],
"yielded": [
244
],
"higher": [
245
],
"percentages": [
246
],
"stained": [
248,
264
],
"fertilization": [
251
],
"rate": [
252
],
"than": [
253
],
"did": [
254
],
"exposure": [
255
],
"propylene": [
257
],
"glycol": [
258
],
"(P=0.002).": [
259
],
"Thawed": [
260
],
"(<1%)": [
267
],
"produced.": [
272
]
},
"apc_list": {
"value": 3670,
"currency": "USD",
"value_usd": 3670,
"provenance": "doaj"
},
"apc_paid": null,
"authorships": [
{
"author_position": "first",
"author": {
"id": "https://openalex.org/A5028366310",
"display_name": "Carmen G. Paniagua\u2010Ch\u00e1vez",
"orcid": "https://orcid.org/0000-0003-2371-6813"
},
"institutions": [
{
"id": "https://openalex.org/I64663401",
"display_name": "Center for Scientific Research and Higher Education at Ensenada",
"ror": "https://ror.org/04znhwb73",
"country_code": "MX",
"type": "facility",
"lineage": [
"https://openalex.org/I64663401"
]
}
],
"countries": [
"MX"
],
"is_corresponding": false,
"raw_author_name": "Carmen G Paniagua-Ch\u00e1vez",
"raw_affiliation_strings": [
"Centro de Investigaci\u00f3n Cient\u00edfica y de Educaci\u00f3n Superior de Ensenada, Departamento de Acuicultura, Km. 107 Carretera Tijuana-Ensenada, Apartado Postal 2732, Ensenada, Baja California M\u00e9xico 22800, Mexico. cpaniagu@cicse.mx"
],
"affiliations": [
{
"raw_affiliation_string": "Centro de Investigaci\u00f3n Cient\u00edfica y de Educaci\u00f3n Superior de Ensenada, Departamento de Acuicultura, Km. 107 Carretera Tijuana-Ensenada, Apartado Postal 2732, Ensenada, Baja California M\u00e9xico 22800, Mexico. cpaniagu@cicse.mx",
"institution_ids": [
"https://openalex.org/I64663401"
]
}
]
},
{
"author_position": "middle",
"author": {
"id": "https://openalex.org/A5013422490",
"display_name": "Jill A. Jenkins",
"orcid": "https://orcid.org/0000-0002-5087-0894"
},
"institutions": [],
"countries": [],
"is_corresponding": false,
"raw_author_name": "Jill Jenkins",
"raw_affiliation_strings": [],
"affiliations": []
},
{
"author_position": "middle",
"author": {
"id": "https://openalex.org/A5090592280",
"display_name": "Manuel Segovia",
"orcid": "https://orcid.org/0000-0003-4303-5599"
},
"institutions": [],
"countries": [],
"is_corresponding": false,
"raw_author_name": "Manuel Segovia",
"raw_affiliation_strings": [],
"affiliations": []
},
{
"author_position": "last",
"author": {
"id": "https://openalex.org/A5003763787",
"display_name": "Terrence R. Tiersch",
"orcid": "https://orcid.org/0000-0002-7738-1978"
},
"institutions": [],
"countries": [],
"is_corresponding": false,
"raw_author_name": "Terrence R Tiersch",
"raw_affiliation_strings": [],
"affiliations": []
}
],
"best_oa_location": null,
"biblio": {
"volume": "53",
"issue": "1",
"first_page": "128",
"last_page": "138"
},
"citation_normalized_percentile": {
"value": 0.936612,
"is_in_top_1_percent": false,
"is_in_top_10_percent": true
},
"cited_by_api_url": "https://api.openalex.org/works?filter=cites:W1975210975",
"cited_by_count": 57,
"cited_by_percentile_year": {
"min": 94,
"max": 95
},
"concepts": [
{
"id": "https://openalex.org/C68528507",
"wikidata": "https://www.wikidata.org/wiki/Q692267",
"display_name": "Cryoprotectant",
"level": 4,
"score": 0.87541264
},
{
"id": "https://openalex.org/C86803240",
"wikidata": "https://www.wikidata.org/wiki/Q420",
"display_name": "Biology",
"level": 0,
"score": 0.69956756
},
{
"id": "https://openalex.org/C179933525",
"wikidata": "https://www.wikidata.org/wiki/Q1144664",
"display_name": "Cryopreservation",
"level": 3,
"score": 0.6120275
},
{
"id": "https://openalex.org/C2781087480",
"wikidata": "https://www.wikidata.org/wiki/Q17145",
"display_name": "Sperm",
"level": 2,
"score": 0.58822584
},
{
"id": "https://openalex.org/C2776364969",
"wikidata": "https://www.wikidata.org/wiki/Q107411",
"display_name": "Oyster",
"level": 2,
"score": 0.5558904
},
{
"id": "https://openalex.org/C2776845067",
"wikidata": "https://www.wikidata.org/wiki/Q542708",
"display_name": "Crassostrea",
"level": 3,
"score": 0.53361607
},
{
"id": "https://openalex.org/C2775934118",
"wikidata": "https://www.wikidata.org/wiki/Q424169",
"display_name": "Propidium iodide",
"level": 4,
"score": 0.49062386
},
{
"id": "https://openalex.org/C2776991149",
"wikidata": "https://www.wikidata.org/wiki/Q468415",
"display_name": "Eastern oyster",
"level": 4,
"score": 0.48164764
},
{
"id": "https://openalex.org/C2777632594",
"wikidata": "https://www.wikidata.org/wiki/Q27116486",
"display_name": "Sperm motility",
"level": 3,
"score": 0.44270402
},
{
"id": "https://openalex.org/C16685009",
"wikidata": "https://www.wikidata.org/wiki/Q335057",
"display_name": "Andrology",
"level": 1,
"score": 0.3652663
},
{
"id": "https://openalex.org/C59822182",
"wikidata": "https://www.wikidata.org/wiki/Q441",
"display_name": "Botany",
"level": 1,
"score": 0.23815632
},
{
"id": "https://openalex.org/C95444343",
"wikidata": "https://www.wikidata.org/wiki/Q7141",
"display_name": "Cell biology",
"level": 1,
"score": 0.21613494
},
{
"id": "https://openalex.org/C55493867",
"wikidata": "https://www.wikidata.org/wiki/Q7094",
"display_name": "Biochemistry",
"level": 1,
"score": 0.1743241
},
{
"id": "https://openalex.org/C505870484",
"wikidata": "https://www.wikidata.org/wiki/Q180538",
"display_name": "Fishery",
"level": 1,
"score": 0.10463861
},
{
"id": "https://openalex.org/C196843134",
"wikidata": "https://www.wikidata.org/wiki/Q33196",
"display_name": "Embryo",
"level": 2,
"score": 0.061758637
},
{
"id": "https://openalex.org/C71924100",
"wikidata": "https://www.wikidata.org/wiki/Q11190",
"display_name": "Medicine",
"level": 0,
"score": 0.0
},
{
"id": "https://openalex.org/C190283241",
"wikidata": "https://www.wikidata.org/wiki/Q14599311",
"display_name": "Apoptosis",
"level": 2,
"score": 0.0
},
{
"id": "https://openalex.org/C31573885",
"wikidata": "https://www.wikidata.org/wiki/Q304484",
"display_name": "Programmed cell death",
"level": 3,
"score": 0.0
}
],
"corresponding_author_ids": [],
"corresponding_institution_ids": [],
"countries_distinct_count": 1,
"counts_by_year": [
{
"year": 2024,
"cited_by_count": 1
},
{
"year": 2022,
"cited_by_count": 7
},
{
"year": 2021,
"cited_by_count": 2
},
{
"year": 2020,
"cited_by_count": 3
},
{
"year": 2019,
"cited_by_count": 1
},
{
"year": 2018,
"cited_by_count": 3
},
{
"year": 2017,
"cited_by_count": 4
},
{
"year": 2016,
"cited_by_count": 2
},
{
"year": 2015,
"cited_by_count": 6
},
{
"year": 2014,
"cited_by_count": 7
},
{
"year": 2013,
"cited_by_count": 3
},
{
"year": 2012,
"cited_by_count": 7
}
],
"created_date": "2016-06-24",
"datasets": [],
"display_name": "Assessment of gamete quality for the eastern oyster (Crassostrea virginica) by use of fluorescent dyes",
"doi": "https://doi.org/10.1016/j.cryobiol.2006.05.001",
"fulltext_origin": "ngrams",
"fwci": 2.201,
"grants": [],
"has_fulltext": true,
"id": "https://openalex.org/W1975210975",
"ids": {
"openalex": "https://openalex.org/W1975210975",
"doi": "https://doi.org/10.1016/j.cryobiol.2006.05.001",
"mag": "1975210975",
"pmid": "https://pubmed.ncbi.nlm.nih.gov/16777086"
},
"indexed_in": [
"crossref",
"pubmed"
],
"institutions_distinct_count": 1,
"is_paratext": false,
"is_retracted": false,
"keywords": [
{
"id": "https://openalex.org/keywords/cryoprotectant",
"display_name": "Cryoprotectant",
"score": 0.87541264
},
{
"id": "https://openalex.org/keywords/oocyte-quality",
"display_name": "Oocyte Quality",
"score": 0.502601
},
{
"id": "https://openalex.org/keywords/propidium-iodide",
"display_name": "Propidium iodide",
"score": 0.49062386
},
{
"id": "https://openalex.org/keywords/eastern-oyster",
"display_name": "Eastern oyster",
"score": 0.48164764
}
],
"language": "en",
"locations": [
{
"is_oa": false,
"landing_page_url": "https://doi.org/10.1016/j.cryobiol.2006.05.001",
"pdf_url": null,
"source": {
"id": "https://openalex.org/S87343901",
"display_name": "Cryobiology",
"issn_l": "0011-2240",
"issn": [
"0011-2240",
"1090-2392"
],
"is_oa": false,
"is_in_doaj": false,
"is_core": true,
"host_organization": "https://openalex.org/P4310320990",
"host_organization_name": "Elsevier BV",
"host_organization_lineage": [
"https://openalex.org/P4310320990"
],
"host_organization_lineage_names": [
"Elsevier BV"
],
"type": "journal"
},
"license": null,
"license_id": null,
"version": null,
"is_accepted": false,
"is_published": false
},
{
"is_oa": false,
"landing_page_url": "https://pubmed.ncbi.nlm.nih.gov/16777086",
"pdf_url": null,
"source": {
"id": "https://openalex.org/S4306525036",
"display_name": "PubMed",
"issn_l": null,
"issn": null,
"is_oa": false,
"is_in_doaj": false,
"is_core": false,
"host_organization": "https://openalex.org/I1299303238",
"host_organization_name": "National Institutes of Health",
"host_organization_lineage": [
"https://openalex.org/I1299303238"
],
"host_organization_lineage_names": [
"National Institutes of Health"
],
"type": "repository"
},
"license": null,
"license_id": null,
"version": null,
"is_accepted": false,
"is_published": false
}
],
"locations_count": 2,
"mesh": [
{
"descriptor_ui": "D049896",
"descriptor_name": "Crassostrea",
"qualifier_ui": "Q000502",
"qualifier_name": "physiology",
"is_major_topic": true
},
{
"descriptor_ui": "D015925",
"descriptor_name": "Cryopreservation",
"qualifier_ui": "Q000379",
"qualifier_name": "methods",
"is_major_topic": true
},
{
"descriptor_ui": "D010063",
"descriptor_name": "Ovum",
"qualifier_ui": "",
"qualifier_name": null,
"is_major_topic": true
},
{
"descriptor_ui": "D000818",
"descriptor_name": "Animals",
"qualifier_ui": "",
"qualifier_name": null,
"is_major_topic": false
},
{
"descriptor_ui": "D002463",
"descriptor_name": "Cell Membrane Permeability",
"qualifier_ui": "",
"qualifier_name": null,
"is_major_topic": false
},
{
"descriptor_ui": "D049896",
"descriptor_name": "Crassostrea",
"qualifier_ui": "Q000166",
"qualifier_name": "cytology",
"is_major_topic": false
},
{
"descriptor_ui": "D049896",
"descriptor_name": "Crassostrea",
"qualifier_ui": "",
"qualifier_name": null,
"is_major_topic": false
},
{
"descriptor_ui": "D015925",
"descriptor_name": "Cryopreservation",
"qualifier_ui": "",
"qualifier_name": null,
"is_major_topic": false
},
{
"descriptor_ui": "D003451",
"descriptor_name": "Cryoprotective Agents",
"qualifier_ui": "",
"qualifier_name": null,
"is_major_topic": false
},
{
"descriptor_ui": "D003451",
"descriptor_name": "Cryoprotective Agents",
"qualifier_ui": "Q000633",
"qualifier_name": "toxicity",
"is_major_topic": false
},
{
"descriptor_ui": "D004121",
"descriptor_name": "Dimethyl Sulfoxide",
"qualifier_ui": "",
"qualifier_name": null,
"is_major_topic": false
},
{
"descriptor_ui": "D004121",
"descriptor_name": "Dimethyl Sulfoxide",
"qualifier_ui": "Q000633",
"qualifier_name": "toxicity",
"is_major_topic": false
},
{
"descriptor_ui": "D005260",
"descriptor_name": "Female",
"qualifier_ui": "",
"qualifier_name": null,
"is_major_topic": false
},
{
"descriptor_ui": "D005434",
"descriptor_name": "Flow Cytometry",
"qualifier_ui": "",
"qualifier_name": null,
"is_major_topic": false
},
{
"descriptor_ui": "D005456",
"descriptor_name": "Fluorescent Dyes",
"qualifier_ui": "",
"qualifier_name": null,
"is_major_topic": false
},
{
"descriptor_ui": "D008297",
"descriptor_name": "Male",
"qualifier_ui": "",
"qualifier_name": null,
"is_major_topic": false
},
{
"descriptor_ui": "D008564",
"descriptor_name": "Membrane Potentials",
"qualifier_ui": "",
"qualifier_name": null,
"is_major_topic": false
},
{
"descriptor_ui": "D008856",
"descriptor_name": "Microscopy, Fluorescence",
"qualifier_ui": "",
"qualifier_name": null,
"is_major_topic": false
},
{
"descriptor_ui": "D010063",
"descriptor_name": "Ovum",
"qualifier_ui": "Q000187",
"qualifier_name": "drug effects",
"is_major_topic": false
},
{
"descriptor_ui": "D011419",
"descriptor_name": "Propidium",
"qualifier_ui": "",
"qualifier_name": null,
"is_major_topic": false
},
{
"descriptor_ui": "D011419",
"descriptor_name": "Propidium",
"qualifier_ui": "Q000494",
"qualifier_name": "pharmacology",
"is_major_topic": false
},
{
"descriptor_ui": "D013081",
"descriptor_name": "Sperm Motility",
"qualifier_ui": "Q000502",
"qualifier_name": "physiology",
"is_major_topic": false
},
{
"descriptor_ui": "D013081",
"descriptor_name": "Sperm Motility",
"qualifier_ui": "",
"qualifier_name": null,
"is_major_topic": false
},
{
"descriptor_ui": "D013094",
"descriptor_name": "Spermatozoa",
"qualifier_ui": "",
"qualifier_name": null,
"is_major_topic": false
},
{
"descriptor_ui": "D013094",
"descriptor_name": "Spermatozoa",
"qualifier_ui": "Q000187",
"qualifier_name": "drug effects",
"is_major_topic": false
}
],
"ngrams_url": "https://api.openalex.org/works/W1975210975/ngrams",
"open_access": {
"is_oa": false,
"oa_status": "closed",
"oa_url": null,
"any_repository_has_fulltext": false
},
"primary_location": {
"is_oa": false,
"landing_page_url": "https://doi.org/10.1016/j.cryobiol.2006.05.001",
"pdf_url": null,
"source": {
"id": "https://openalex.org/S87343901",
"display_name": "Cryobiology",
"issn_l": "0011-2240",
"issn": [
"0011-2240",
"1090-2392"
],
"is_oa": false,
"is_in_doaj": false,
"is_core": true,
"host_organization": "https://openalex.org/P4310320990",
"host_organization_name": "Elsevier BV",
"host_organization_lineage": [
"https://openalex.org/P4310320990"
],
"host_organization_lineage_names": [
"Elsevier BV"
],
"type": "journal"
},
"license": null,
"license_id": null,
"version": null,
"is_accepted": false,
"is_published": false
},
"primary_topic": {
"id": "https://openalex.org/T10116",
"display_name": "Male Reproductive Health",
"score": 0.9993,
"subfield": {
"id": "https://openalex.org/subfields/2743",
"display_name": "Reproductive Medicine"
},
"field": {
"id": "https://openalex.org/fields/27",
"display_name": "Medicine"
},
"domain": {
"id": "https://openalex.org/domains/4",
"display_name": "Health Sciences"
}
},
"publication_date": "2006-08-01",
"publication_year": 2006,
"referenced_works": [
"https://openalex.org/W1501091531",
"https://openalex.org/W1516629870",
"https://openalex.org/W1518617829",
"https://openalex.org/W155267013",
"https://openalex.org/W1913080457",
"https://openalex.org/W1964868864",
"https://openalex.org/W1971969114",
"https://openalex.org/W1974945118",
"https://openalex.org/W1980181948",
"https://openalex.org/W1983710645",
"https://openalex.org/W1989504788",
"https://openalex.org/W2001965015",
"https://openalex.org/W2023779508",
"https://openalex.org/W2029096531",
"https://openalex.org/W2038742295",
"https://openalex.org/W2044789787",
"https://openalex.org/W2044852315",
"https://openalex.org/W2058625482",
"https://openalex.org/W2085605635",
"https://openalex.org/W2086628112",
"https://openalex.org/W2090369910",
"https://openalex.org/W2097997168",
"https://openalex.org/W2111735005",
"https://openalex.org/W2124327021",
"https://openalex.org/W2131391649",
"https://openalex.org/W2137815839",
"https://openalex.org/W2150097020",
"https://openalex.org/W2152697478",
"https://openalex.org/W2280988113",
"https://openalex.org/W2317139548",
"https://openalex.org/W2441190971",
"https://openalex.org/W2496801411",
"https://openalex.org/W3189343543",
"https://openalex.org/W572556395"
],
"referenced_works_count": 34,
"related_works": [
"https://openalex.org/W3190431596",
"https://openalex.org/W3082433135",
"https://openalex.org/W3047558598",
"https://openalex.org/W2618600520",
"https://openalex.org/W2409624424",
"https://openalex.org/W2172475889",
"https://openalex.org/W2107707071",
"https://openalex.org/W2074586757",
"https://openalex.org/W2059667255",
"https://openalex.org/W1997418417"
],
"sustainable_development_goals": [
{
"id": "https://metadata.un.org/sdg/14",
"score": 0.62,
"display_name": "Life below water"
}
],
"title": "Assessment of gamete quality for the eastern oyster (Crassostrea virginica) by use of fluorescent dyes",
"topics": [
{
"id": "https://openalex.org/T10116",
"display_name": "Male Reproductive Health",
"score": 0.9993,
"subfield": {
"id": "https://openalex.org/subfields/2743",
"display_name": "Reproductive Medicine"
},
"field": {
"id": "https://openalex.org/fields/27",
"display_name": "Medicine"
},
"domain": {
"id": "https://openalex.org/domains/4",
"display_name": "Health Sciences"
}
},
{
"id": "https://openalex.org/T10364",
"display_name": "Fertility Preservation in Cancer Patients",
"score": 0.9992,
"subfield": {
"id": "https://openalex.org/subfields/2739",
"display_name": "Public Health, Environmental and Occupational Health"
},
"field": {
"id": "https://openalex.org/fields/27",
"display_name": "Medicine"
},
"domain": {
"id": "https://openalex.org/domains/4",
"display_name": "Health Sciences"
}
},
{
"id": "https://openalex.org/T11505",
"display_name": "Reproductive Physiology and Endocrine Disruption in Fish",
"score": 0.9937,
"subfield": {
"id": "https://openalex.org/subfields/1314",
"display_name": "Physiology"
},
"field": {
"id": "https://openalex.org/fields/13",
"display_name": "Biochemistry, Genetics and Molecular Biology"
},
"domain": {
"id": "https://openalex.org/domains/1",
"display_name": "Life Sciences"
}
}
],
"type": "article",
"type_crossref": "journal-article",
"updated_date": "2024-08-13T05:19:23.501877",
"versions": []
}
}