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Diseases are typically named or described based on macroscopic changes, but broad signs of coral distress such as tissue loss or discoloration are unlikely to be specific to a particular pathogen. For example, there appear to be multiple diseases that manifest the rapid tissue loss that characterizes \u2018white plague.\u2019 PhyloChip\u2122 G3 microarrays were used to compare the bacterial community composition of both healthy and white plague-like diseased corals. Samples of lobed star coral (Orbicella annularis, formerly of the genus\u00a0Montastraea\u00a0[1]) were collected from two geographically distinct areas, Dry Tortugas National Park and Virgin Islands National Park, to determine if there were biogeographic differences between the diseases. In fact, all diseased samples clustered together, however there was no consistent link to\u00a0Aurantimonas coralicida, which has been described as the causative agent of white plague type II. The microarrays revealed a large amount of bacterial heterogeneity within the healthy corals and less diversity in the diseased corals. Gram-positive bacterial groups (Actinobacteria, Firmicutes) comprised a greater proportion of the operational taxonomic units (OTUs) unique to healthy samples. Diseased samples were enriched in OTUs from the families Corynebacteriaceae, Lachnospiraceae, Rhodobacteraceae, and Streptococcaceae. Much previous coral disease work has used clone libraries, which seem to be methodologically biased toward recovery of Gram-negative bacterial sequences and may therefore have missed the importance of Gram-positive groups. The PhyloChip\u2122 data presented here provide a broader characterization of the bacterial community changes that occur within\u00a0Orbicella annularis\u00a0during the shift from a healthy to diseased state.", "description": "e79801; 10 p.", "publisher": { "@type": "Organization", "name": "PLOS" }, "author": [ { "@type": "Person", "name": "Kellogg, Christina A. ckellogg@usgs.gov", "givenName": "Christina A.", "familyName": "Kellogg", "email": "ckellogg@usgs.gov", "identifier": { "@type": "PropertyValue", "propertyID": "ORCID", "value": "0000-0002-6492-9455", "url": "https://orcid.org/0000-0002-6492-9455" }, "affiliation": [ { "@type": "Organization", "name": "Office of the AD Ecosystems", "url": "https://www.usgs.gov/mission-areas/ecosystems" }, { "@type": "Organization", "name": "St. Petersburg Coastal and Marine Science Center", "url": "https://www.usgs.gov/centers/spcmsc" } ] }, { "@type": "Person", "name": "Zawada, David G. dzawada@usgs.gov", "givenName": "David G.", "familyName": 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