{"@context": "https://schema.org", "@type": "CreativeWork", "additionalType": "USGS Numbered Series", "name": "A methodology for quantifying trace elements in the exoskeletons of Florida stone crab (Menippe mercenaria) larvae using inductively coupled plasma optical emission spectrometry (ICP\u2013OES) ", "identifier": [{"@type": "PropertyValue", "propertyID": "USGS Publications Warehouse IndexID", "value": "ofr20161148", "url": "https://pubs.usgs.gov/publication/ofr20161148"}, {"@type": "PropertyValue", "propertyID": "USGS Publications Warehouse Internal ID", "value": 70178286}, {"@type": "PropertyValue", "propertyID": "DOI", "value": "10.3133/ofr20161148", "url": "https://doi.org/10.3133/ofr20161148"}], "inLanguage": "en", "isPartOf": [{"@type": "CreativeWorkSeries", "name": "Open-File Report"}], "datePublished": "2016", "dateModified": "2016-11-23", "abstract": "The larvae of the Florida stone crab, Menippe mercenaria, migrate through a variety of habitats as they develop and, therefore, experience a broad range of environmental conditions through ontogeny. Environmental variability experienced by the larvae may result in distinct elemental signatures within the exoskeletons, which could provide a tool for tracking the environmental history of larval stone crab populations. A method was developed to examine trace-element ratios, specifically magnesium-to-calcium (Mg/Ca) and strontium-to-calcium (Sr/Ca) ratios, in the exoskeletons of M. mercenaria larvae. Two developmental stages of stone crab larvae were analyzed\u2014stage III and stage V. Specimens were reared in a laboratory environment under stable conditions to quantify the average ratios of Mg/Ca and Sr/Ca of larval stone crab exoskeletons and to determine if the ratios differed through ontogeny. The elemental compositions (Ca, Mg, and Sr) in samples of stage III larvae (n = 50 per sample) from 11 different broods (mean Sr/Ca = 5.916 \u00b1 0.161 millimole per mole [mmol mol\u22121]; mean Mg/Ca = 218.275 \u00b1 59.957 mmol mol\u22121) and stage V larvae (n = 10 per sample) from 12 different broods (mean Sr/Ca = 6.110 \u00b1 0.300 mmol mol\u22121; mean Mg/Ca = 267.081 \u00b1 67.211 mmol mol\u20131) were measured using inductively coupled plasma optical emission spectrometry (ICP\u2013OES). The ratio of Sr/Ca significantly increased from stage III to stage V larvae, suggesting an ontogenic shift in Sr/Ca ratios between larval stages. The ratio of Mg/Ca did not change significantly between larval stages, but variability among broods was high. The method used to examine the trace-element ratios provided robust, highly reproducible estimates of Sr/Ca and Mg/Ca ratios in the larvae of M. mercenaria, demonstrating that ICP\u2013OES can be used to determine the trace-element composition of chitinous organisms like the Florida stone crab.", "description": "vi, 12 p.", "publisher": {"@type": "Organization", "name": "U.S. Geological Survey"}, "author": [{"@type": "Person", "name": "Flannery, Jennifer A. jflannery@usgs.gov", "givenName": "Jennifer A.", "familyName": "Flannery", "email": "jflannery@usgs.gov", "identifier": {"@type": "PropertyValue", "propertyID": "ORCID", "value": "0000-0002-1692-2662", "url": "https://orcid.org/0000-0002-1692-2662"}, "affiliation": [{"@type": "Organization", "name": "St. Petersburg Coastal and Marine Science Center", "url": "https://www.usgs.gov/centers/spcmsc"}]}, {"@type": "Person", "name": "Gravinese, Philip M.", "givenName": "Philip M.", "familyName": "Gravinese"}, {"@type": "Person", "name": "Toth, Lauren T. ltoth@usgs.gov", "givenName": "Lauren T.", "familyName": "Toth", "email": "ltoth@usgs.gov", "affiliation": [{"@type": "Organization", "name": "St. Petersburg Coastal and Marine Science Center", "url": "https://www.usgs.gov/centers/spcmsc"}]}], "funder": [{"@type": "Organization", "name": "St. Petersburg Coastal and Marine Science Center", "url": "https://www.usgs.gov/centers/spcmsc"}]}